{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T07:15:24Z","timestamp":1775200524881,"version":"3.50.1"},"reference-count":79,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The increasing occurrence of illegal urban waste dumping represents a growing environmental concern due to the accumulation of heavy metals in soils and their long-term impact on soil health and microbial communities.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study investigated indigenous\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      isolates from 12 contaminated soils collected in Southern Italy, aiming to evaluate their adaptive responses to abiotic stress and their potential for bioremediation. A total of one hundred isolates were obtained and screened for growth under heavy-metal stress conditions (Cu, Pb, Cr, Zn, As). Growth-based tolerance assays were used as a preliminary screening, and only isolates showing measurable removal capacity were further characterized using ICP\u2013OES analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and discussion<\/jats:title>\n                    <jats:p>\n                      Selected strains exhibited broad tolerance across different single-metal exposures, with removal efficiencies exceeding 65% for Cr, Zn, Cu, and As, and up to 90% for Pb. These isolates also demonstrated robust growth under osmotic and acidic stress, and maintained viability at low temperatures, suggesting a high level of ecological adaptability. Phylogenetic analysis based on 16S rRNA sequencing confirmed their affiliation within the\n                      <jats:italic>P. fluorescens<\/jats:italic>\n                      and\n                      <jats:italic>P. putida<\/jats:italic>\n                      groups, taxa known for their metabolic flexibility and stress resistance. The convergence of high removal efficiency, stress tolerance, and phylogenetic diversity indicates that these isolates represent strong candidates for bioaugmentation in metal-polluted soils. Future studies will focus on co-exposure experiments with mixed metals to evaluate synergistic and antagonistic effects and to validate their performance under complex contamination scenarios.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2025.1731818","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T06:36:24Z","timestamp":1765953384000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Phenotypic and functional characterization of soil Pseudomonas strains reveals multi-metal tolerance and bioremediation potential"],"prefix":"10.3389","volume":"16","author":[{"given":"Alessandro","family":"De Santis","sequence":"first","affiliation":[]},{"given":"Antonio","family":"Bevilacqua","sequence":"additional","affiliation":[]},{"given":"Sara","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[]},{"given":"Marta","family":"Laranjo","sequence":"additional","affiliation":[]},{"given":"Matteo","family":"Francavilla","sequence":"additional","affiliation":[]},{"given":"Mauro","family":"Marone","sequence":"additional","affiliation":[]},{"given":"Maria Rosaria","family":"Corbo","sequence":"additional","affiliation":[]},{"given":"Milena","family":"Sinigaglia","sequence":"additional","affiliation":[]}],"member":"1965","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"42","DOI":"10.3390\/toxics9030042","article-title":"Heavy metals and pesticides toxicity in agricultural soil and plants: ecological risks and human health implications","volume":"9","author":"Alengebawy","year":"2021","journal-title":"Toxics"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"4253","DOI":"10.3390\/polym14204253","article-title":"Role of exopolysaccharides of Pseudomonas in heavy metal removal and other remediation strategies","volume":"14","author":"Bal\u00edkov\u00e1","year":"2022","journal-title":"Polymers"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"194","DOI":"10.3389\/fmicb.2012.00194","article-title":"Selection of yeasts as starter cultures for table olives: a step-by-step procedure","volume":"3","author":"Bevilacqua","year":"2009","journal-title":"Front. 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