{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:54:36Z","timestamp":1773392076093,"version":"3.50.1"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Aging Neurosci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Alzheimer\u2019s disease (AD) remains one of the greatest challenges in neurodegenerative research, largely due to the toxic aggregation of the \u03b2-amyloid (A\u03b2) peptide and the difficulty of delivering therapeutic compounds across the blood-brain barrier (BBB).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This work explored\n                      <jats:italic>Ulva rigida<\/jats:italic>\n                      (\n                      <jats:italic>U. rigida<\/jats:italic>\n                      ), a green macroalga rich in bioactive peptides, as a natural source of anti-amyloidogenic and antioxidant agents.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      First, the peptides extraction process was optimized by design of experiments (DoE). Peptides extracted and hydrolyzed under optimized conditions showed antioxidant capacity (2.51 \u00b1 0.18 \u03bcmol\n                      <jats:sub>Trolox equivalent<\/jats:sub>\n                      \/ mg\n                      <jats:sub>hydrolyzed protein<\/jats:sub>\n                      ), and the fraction of peptides with a molecular weight lower than 3 kDa was able to reduce the aggregation of A\u03b2\n                      <jats:sub>\n                        <jats:italic>1\u201342<\/jats:italic>\n                      <\/jats:sub>\n                      peptide\n                      <jats:italic>in vitro<\/jats:italic>\n                      by around 60%. To overcome the issue of limited bioavailability, we encapsulated these peptides in solid lipid nanoparticles (SLNs). The developed nanoformulation displayed a size of 158 \u00b1 14 nm, narrow polydispersity, and near-neutral zeta potential (\u22124.7 \u00b1 0.6 mV), with an encapsulation efficiency (EE) of 54.1 \u00b1 0.1% and suitable stability under simulated gastrointestinal and storage conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      By combining biological activity with a delivery platform, this study highlights the potential of\n                      <jats:italic>Ulva rigida<\/jats:italic>\n                      as a source of bioactive peptides with the potential to delay the progression of AD.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fnagi.2026.1778124","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:48:58Z","timestamp":1773384538000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Solid lipid nanoparticles as carriers for Ulva rigida peptide extract with demonstrated anti-\u03b2-amyloid and antioxidant properties"],"prefix":"10.3389","volume":"18","author":[{"given":"T\u00e2nia G.","family":"Tavares","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]}]},{"given":"Iva","family":"Carvalho","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]}]},{"given":"Maria Jo\u00e3o","family":"Ramalho","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]}]},{"given":"St\u00e9phanie","family":"Andrade","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]}]},{"given":"Maria Carmo","family":"Pereira","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]}]},{"given":"Joana A.","family":"Loureiro","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]},{"name":"Department of Mechanical Engineering, Faculty of Engineering, University of Porto","place":["Porto, Portugal"]}]}],"member":"1965","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"6685","DOI":"10.3390\/ijms12106685","article-title":"Effect of peptide size on antioxidant properties of African yam bean seed (Sphenostylis stenocarpa) protein hydrolysate fractions.","volume":"12","author":"Ajibola","year":"2011","journal-title":"Int. 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