{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T15:51:38Z","timestamp":1773849098895,"version":"3.50.1"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T00:00:00Z","timestamp":1696204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p><jats:bold>Introduction:<\/jats:bold> Management of acute myocardial infarction (MI) mandates careful optimization of volemia, which can be challenging due to the inherent risk of congestion. Increased myocardial compliance in response to stretching, known as stretch-induced compliance (SIC), has been recently characterized and partly ascribed to cGMP\/cGMP-dependent protein kinase (PKG)-related pathways. We hypothesized that SIC would be impaired in MI but restored by activation of PKG, thereby enabling a better response to volume loading in MI.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> We conducted experiments in <jats:italic>ex vivo<\/jats:italic> rabbit right ventricular papillary muscles under ischemic and non-ischemic conditions as well as pressure\u2013volume hemodynamic evaluations in experimental <jats:italic>in vivo<\/jats:italic> MI induced by left anterior descending artery ligation in rats.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Acutely stretching muscles <jats:italic>ex vivo<\/jats:italic> yielded increased compliance over the next 15\u00a0min, but not under ischemic conditions. PKG agonists, but not PKC agonists, were able to partially restore SIC in ischemic muscles. A similar effect was observed with phosphodiesterase-5 inhibitor (PDE5<jats:sub>i<\/jats:sub>) sildenafil, which was amplified by joint B-type natriuretic peptide or nitric oxide donor administration. <jats:italic>In vivo<\/jats:italic> translation revealed that volume loading after MI only increased cardiac output in rats infused with PDE5<jats:sub>i<\/jats:sub>. Contrarily to vehicle, sildenafil-treated rats showed a clear increase in myocardial compliance upon volume loading.<\/jats:p><jats:p><jats:bold>Discussion:<\/jats:bold> Our results suggest that ischemia impairs the adaptive myocardial response to acute stretching and that this may be partly prevented by pharmacological manipulation of the cGMP\/PKG pathway, namely, with PDE5<jats:sub>i<\/jats:sub>. Further studies are warranted to further elucidate the potential of this intervention in the clinical setting of acute myocardial ischemia.<\/jats:p>","DOI":"10.3389\/fphys.2023.1271698","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T15:26:44Z","timestamp":1696433204000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Myocardial stretch-induced compliance is abrogated under ischemic conditions and restored by cGMP\/PKG-related pathways"],"prefix":"10.3389","volume":"14","author":[{"given":"Andr\u00e9 M.","family":"Leite-Moreira","sequence":"first","affiliation":[]},{"given":"Jo\u00e3o","family":"Almeida-Coelho","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o S.","family":"Neves","sequence":"additional","affiliation":[]},{"given":"Ricardo","family":"Castro-Ferreira","sequence":"additional","affiliation":[]},{"given":"Ricardo","family":"Ladeiras-Lopes","sequence":"additional","affiliation":[]},{"given":"Adelino F.","family":"Leite-Moreira","sequence":"additional","affiliation":[]},{"given":"Andr\u00e9 P.","family":"Louren\u00e7o","sequence":"additional","affiliation":[]}],"member":"1965","published-online":{"date-parts":[[2023,10,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1016\/j.ijcard.2016.08.264","article-title":"sGC-cGMP-PKG pathway stimulation protects the healthy but not the failing right ventricle of rats against ischemia and reperfusion injury","volume":"223","author":"Andersen","year":"2016","journal-title":"Int. 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