{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T11:31:43Z","timestamp":1763811103323,"version":"build-2065373602"},"reference-count":64,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Clinical Medicine, Aalborg University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computation"],"abstract":"<jats:p>Crohn disease (CD) is a type of inflammatory bowel disease that causes inflammation in the digestive tract. Cases of CD are increasing worldwide, calling for more research to elucidate the pathogenesis of CD. For this purpose, the usage of the RNA-sequencing (RNA-seq) technique is increasingly appreciated, as it captures RNA expression patterns at a particular time point in a high-throughput manner. Although many RNA-seq datasets are generated from CD patients and compared to those of healthy donors, most of these datasets are analyzed only for protein-coding genes, leaving non-coding RNAs (ncRNAs) undiscovered. Long non-coding RNAs (lncRNAs) are any ncRNAs that are longer than 200 nucleotides. Interest in studying lncRNAs is increasing rapidly, as lncRNAs bind other macromolecules (DNA, RNA, and\/or proteins) to finetune signaling pathways. To fill the gap in knowledge about lncRNAs in CD, we performed secondary analysis of published RNA-seq data of CD patients compared to healthy donors to identify lncRNA genes and their expression changes. To further facilitate lncRNA research in CD, we built a web database, CrohnDB, to provide a one-stop-shop for expression profiling of protein-coding and lncRNA genes in CD patients compared to healthy donors.<\/jats:p>","DOI":"10.3390\/computation11060105","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T01:36:28Z","timestamp":1684978588000},"page":"105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["CrohnDB: A Web Database for Expression Profiling of Protein-Coding and Long Non-Coding RNA Genes in Crohn Disease"],"prefix":"10.3390","volume":"11","author":[{"given":"Rebecca","family":"Distefano","sequence":"first","affiliation":[{"name":"Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7992-8885","authenticated-orcid":false,"given":"Mirolyuba","family":"Ilieva","sequence":"additional","affiliation":[{"name":"Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, DK-2450 Copenhagen SV, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens Hedelund","family":"Madsen","sequence":"additional","affiliation":[{"name":"Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, DK-2450 Copenhagen SV, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4787-8067","authenticated-orcid":false,"given":"Shizuka","family":"Uchida","sequence":"additional","affiliation":[{"name":"Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, DK-2450 Copenhagen SV, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1001\/jama.2020.18936","article-title":"Management of Crohn Disease: A Review","volume":"325","author":"Cushing","year":"2021","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41572-020-0156-2","article-title":"Crohn\u2019s disease","volume":"6","author":"Roda","year":"2020","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.disamonth.2017.07.001","article-title":"A comprehensive review and update on Crohn\u2019s disease","volume":"64","author":"Gajendran","year":"2018","journal-title":"Dis. Mon."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1016\/S0140-6736(17)32448-0","article-title":"Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: A systematic review of population-based studies","volume":"390","author":"Ng","year":"2017","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6664","DOI":"10.1038\/s41467-021-26992-4","article-title":"Psychological stress impairs IL22-driven protective gut mucosal immunity against colonising pathobionts","volume":"12","author":"Shaler","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"432","DOI":"10.3389\/fped.2019.00432","article-title":"Stress Triggers Flare of Inflammatory Bowel Disease in Children and Adults","volume":"7","author":"Sun","year":"2019","journal-title":"Front. Pediatr."},{"key":"ref_7","first-page":"51","article-title":"The Role of Diet in Inflammatory Bowel Disease","volume":"12","author":"Lewis","year":"2016","journal-title":"Gastroenterol. Hepatol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Garza-Hernandez, D., Sepulveda-Villegas, M., Garcia-Pelaez, J., Aguirre-Gamboa, R., Lakatos, P.L., Estrada, K., Martinez-Vazquez, M., and Trevino, V. (2022). A systematic review and functional bioinformatics analysis of genes associated with Crohn\u2019s disease identify more than 120 related genes. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08491-y"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.1038\/s41467-021-22306-w","article-title":"High-throughput fitness screening and transcriptomics identify a role for a type IV secretion system in the pathogenesis of Crohn\u2019s disease-associated Escherichia coli","volume":"12","author":"Elhenawy","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1038\/s41467-021-22164-6","article-title":"Single-cell analyses of Crohn\u2019s disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions","volume":"12","author":"Jaeger","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1093\/ecco-jcc\/jjy190","article-title":"High-Throughput Identification of the Plasma Proteomic Signature of Inflammatory Bowel Disease","volume":"13","author":"Brodmerkel","year":"2019","journal-title":"J. Crohns Colitis"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"374","DOI":"10.5217\/ir.2018.16.3.374","article-title":"Delineating inflammatory bowel disease through transcriptomic studies: Current review of progress and evidence","volume":"16","author":"Chan","year":"2018","journal-title":"Intest. Res."},{"key":"ref_13","first-page":"366","article-title":"So much \u201cjunk\u201d DNA in our genome","volume":"23","author":"Ohno","year":"1972","journal-title":"Brookhaven Symp. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"344","DOI":"10.3390\/genes3030344","article-title":"The human transcriptome: An unfinished story","volume":"3","author":"Pertea","year":"2012","journal-title":"Genes"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"R70","DOI":"10.1186\/gb-2013-14-7-r70","article-title":"Transcriptome analysis of human tissues and cell lines reveals one dominant transcript per gene","volume":"14","author":"Frankish","year":"2013","journal-title":"Genome Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3389\/fgene.2015.00002","article-title":"Non-coding RNA: What is functional and what is junk?","volume":"6","author":"Palazzo","year":"2015","journal-title":"Front. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/s41580-020-00315-9","article-title":"Gene regulation by long non-coding RNAs and its biological functions","volume":"22","author":"Statello","year":"2021","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41467-019-13688-z","article-title":"In vivo functional analysis of non-conserved human lncRNAs associated with cardiometabolic traits","volume":"11","author":"Ruan","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"C1003","DOI":"10.1152\/ajpcell.00232.2022","article-title":"Noncoding RNAs and RNA-binding proteins: Emerging governors of liver physiology and metabolic diseases","volume":"323","author":"Sommerauer","year":"2022","journal-title":"Am. J. Physiol. Cell. Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zhang, X., Wang, W., Zhu, W., Dong, J., Cheng, Y., Yin, Z., and Shen, F. (2019). Mechanisms and Functions of Long Non-Coding RNAs at Multiple Regulatory Levels. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20225573"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1186\/s13059-017-1348-2","article-title":"The multidimensional mechanisms of long noncoding RNA function","volume":"18","author":"Marchese","year":"2017","journal-title":"Genome Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1101\/gr.132159.111","article-title":"The GENCODE v7 catalog of human long noncoding RNAs: Analysis of their gene structure, evolution, and expression","volume":"22","author":"Derrien","year":"2012","journal-title":"Genome Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1038\/nrg3074","article-title":"Non-coding RNAs in human disease","volume":"12","author":"Esteller","year":"2011","journal-title":"Nat. Rev. Genet."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/978-1-4939-7471-9_6","article-title":"The Emerging Role of Long Noncoding RNAs in Human Disease","volume":"1706","author":"DiStefano","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-018-0188-9","article-title":"The emerging role of lncRNAs in inflammatory bowel disease","volume":"50","author":"Yarani","year":"2018","journal-title":"Exp. Mol. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13073-015-0162-2","article-title":"Transcriptomic landscape of lncRNAs in inflammatory bowel disease","volume":"7","author":"Mirza","year":"2015","journal-title":"Genome Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ilieva, M., Dao, J., Miller, H.E., Madsen, J.H., Bishop, A.J.R., Kauppinen, S., and Uchida, S. (2022). Systematic Analysis of Long Non-Coding RNA Genes in Nonalcoholic Fatty Liver Disease. Noncoding RNA, 8.","DOI":"10.3390\/ncrna8040056"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ilieva, M., Miller, H.E., Agarwal, A., Paulus, G.K., Madsen, J.H., Bishop, A.J.R., Kauppinen, S., and Uchida, S. (2022). FibroDB: Expression Analysis of Protein-Coding and Long Non-Coding RNA Genes in Fibrosis. Noncoding RNA, 8.","DOI":"10.3390\/ncrna8010013"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"D19","DOI":"10.1093\/nar\/gkq1019","article-title":"The sequence read archive","volume":"39","author":"Leinonen","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_33","unstructured":"Wickham, H. (2016). Use R!, Springer International Publishing."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Sherman","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/nar\/gkn923","article-title":"Bioinformatics enrichment tools: Paths toward the comprehensive functional analysis of large gene lists","volume":"37","author":"Sherman","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_36","unstructured":"Winston, C., Joe, C., JJ, A., Carson, S., Barret, S., Yihui, X., Jeff, A., Jonathan, M.P., Alan, D., and Barbara, B. (2023, March 21). Shiny: Web Application Framework for R. Available online: https:\/\/shiny.rstudio.com\/."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Sievert, C. (2022, May 22). Interactive Web-Based Data Visualization with R, Plotly, and Shiny; Chapman and Hall\/CRC. 2020. Available online: https:\/\/plotly-r.com.","DOI":"10.1201\/9780429447273"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"W191","DOI":"10.1093\/nar\/gkz369","article-title":"g:Profiler: A web server for functional enrichment analysis and conversions of gene lists (2019 update)","volume":"47","author":"Raudvere","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_39","first-page":"100141","article-title":"clusterProfiler 4.0: A universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation"},{"key":"ref_40","unstructured":"Yu, G. (2023, March 21). Enrichplot: Visualization of Functional Enrichment Result. 2022. Available online: https:\/\/yulab-smu.top\/biomedical-knowledge-mining-book\/."},{"key":"ref_41","unstructured":"Larsson, J. (2022). eulerr: Area-Proportional Euler and Venn Diagrams with Ellipses, R Foundation for Statistical Computing. R package version 7.0.0."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1093\/bioinformatics\/btx364","article-title":"UpSetR: An R package for the visualization of intersecting sets and their properties","volume":"33","author":"Conway","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1055\/s-0033-1348046","article-title":"Colonic crohn disease","volume":"26","author":"Hedrick","year":"2013","journal-title":"Clin. Colon. Rectal Surg."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.bbagrm.2018.11.004","article-title":"Coregulatory long non-coding RNA and protein-coding genes in serum starved cells","volume":"1862","author":"Wang","year":"2019","journal-title":"Biochim. Biophys. Acta Gene Regul. Mech."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.1097\/MIB.0000000000000478","article-title":"MicroRNAs Classify Different Disease Behavior Phenotypes of Crohn\u2019s Disease and May Have Prognostic Utility","volume":"21","author":"Peck","year":"2015","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1111\/1759-7714.13325","article-title":"Unique metastasis-associated lncRNA signature optimizes prediction of tumor relapse in lung adenocarcinoma","volume":"11","author":"Zhang","year":"2020","journal-title":"Thorac. Cancer"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1038\/nm.2807","article-title":"Mechanisms of fibrosis: Therapeutic translation for fibrotic disease","volume":"18","author":"Wynn","year":"2012","journal-title":"Nat. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3389\/fimmu.2012.00071","article-title":"Cellular and molecular mechanisms of chronic inflammation-associated organ fibrosis","volume":"3","author":"Ueha","year":"2012","journal-title":"Front. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.matbio.2017.11.010","article-title":"Inflammation and fibrosis","volume":"68\u201369","author":"Mack","year":"2018","journal-title":"Matrix Biol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Li Yim, A.Y.F., de Bruyn, J.R., Duijvis, N.W., Sharp, C., Ferrero, E., de Jonge, W.J., Wildenberg, M.E., Mannens, M., Buskens, C.J., and D\u2019Haens, G.R. (2018). A distinct epigenetic profile distinguishes stenotic from non-inflamed fibroblasts in the ileal mucosa of Crohn\u2019s disease patients. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0209656"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"108877","DOI":"10.1016\/j.biopha.2019.108877","article-title":"HBx-related long non-coding RNA 01152 promotes cell proliferation and survival by IL-23 in hepatocellular carcinoma","volume":"115","author":"Chen","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1038\/s41419-021-04106-8","article-title":"Correction: LINC01152 upregulates MAML2 expression to modulate the progression of glioblastoma multiforme via Notch signaling pathway","volume":"12","author":"Wu","year":"2021","journal-title":"Cell. Death Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"787884","DOI":"10.3389\/fgene.2022.787884","article-title":"Identification and Validation of a Ferroptosis-Related Long Non-Coding RNA (FRlncRNA) Signature to Predict Survival Outcomes and the Immune Microenvironment in Patients with Clear Cell Renal Cell Carcinoma","volume":"13","author":"Zhou","year":"2022","journal-title":"Front. Genet."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"8849977","DOI":"10.1155\/2021\/8849977","article-title":"A Risk Signature with Autophagy-Related Long Noncoding RNAs for Predicting the Prognosis of Clear Cell Renal Cell Carcinoma: Based on the TCGA Database and Bioinformatics","volume":"2021","author":"Xuan","year":"2021","journal-title":"Dis. Markers"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1111\/cas.15384","article-title":"The HOXC-AS2\/miR-876-5p\/HKDC1 axis regulates endometrial cancer progression in a high glucose-related tumor microenvironment","volume":"113","author":"Guo","year":"2022","journal-title":"Cancer Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e23651","DOI":"10.1002\/jcla.23651","article-title":"Novel long noncoding RNA LINC02323 promotes cell growth and migration of ovarian cancer via TGF-beta receptor 1 by miR-1343-3p","volume":"35","author":"Li","year":"2021","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2506","DOI":"10.1111\/1759-7714.13562","article-title":"Novel long non-coding RNA LINC02323 promotes epithelial-mesenchymal transition and metastasis via sponging miR-1343-3p in lung adenocarcinoma","volume":"11","author":"Zhang","year":"2020","journal-title":"Thorac. Cancer"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"7601","DOI":"10.2147\/OTT.S216134","article-title":"Positive feedback loop of lncRNA HOXC-AS2\/miR-876-5p\/ZEB1 to regulate EMT in glioma","volume":"12","author":"Dong","year":"2019","journal-title":"OncoTargets Ther."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/1423-0127-20-87","article-title":"LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn\u2019s disease","volume":"20","author":"Qiao","year":"2013","journal-title":"J. Biomed. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"829335","DOI":"10.3389\/fimmu.2022.829335","article-title":"ISR8\/IRF1-AS1 Is Relevant for IFNalpha and NF-kappaB Responses","volume":"13","author":"Barriocanal","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3838","DOI":"10.3748\/wjg.v28.i29.3838","article-title":"High-fat diet aggravates colitis via mesenteric adipose tissue derived exosome metastasis-associated lung adenocarcinoma transcript 1","volume":"28","author":"Chen","year":"2022","journal-title":"World J. Gastroenterol."},{"key":"ref_62","first-page":"2331","article-title":"LINC01272 activates epithelial-mesenchymal transition through miR-153-5p in Crohn\u2019s disease","volume":"14","author":"Fang","year":"2022","journal-title":"Am. J. Transl. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.biocel.2013.10.009","article-title":"Life without A tail: New formats of long noncoding RNAs","volume":"54","author":"Zhang","year":"2014","journal-title":"Int. J. Biochem. Cell. Biol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1172\/JCI93555","article-title":"Fibroblast heterogeneity: Implications for human disease","volume":"128","author":"Lynch","year":"2018","journal-title":"J. Clin. Investig."}],"container-title":["Computation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-3197\/11\/6\/105\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:41:05Z","timestamp":1760125265000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-3197\/11\/6\/105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,24]]},"references-count":64,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["computation11060105"],"URL":"https:\/\/doi.org\/10.3390\/computation11060105","relation":{},"ISSN":["2079-3197"],"issn-type":[{"type":"electronic","value":"2079-3197"}],"subject":[],"published":{"date-parts":[[2023,5,24]]}}}