{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:40:21Z","timestamp":1759970421133,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union","award":["CUP B33C22000710006"],"award-info":[{"award-number":["CUP B33C22000710006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computation"],"abstract":"<jats:p>Asymmetric cove-edged graphene nano-ribbons were employed as metallic electrodes in a hybrid gap device structure with zig-zag graphene nano-ribbons terminations for amino acid recognition and peptide sequencing. On a theoretical basis, amino acid recognition is attained by calculating, using the non equilibrium Green function scheme based on density functional theory, the transversal tunnelling current flowing across the gap device during the peptide translocation through the device. The reliability and robustness of this sequencing method versus relevant operations parameters, such as the bias, the gap size, and small perturbations of the atomistic structures, are studied for the paradigmatic case of Pro-Ser model peptide. I evidence that the main features of the tunnelling signal, that allow the recognition, survive for all of the operational conditions explored. I also evidence a sort of geometrical selective sensitivity of the hybrid cove-edged graphene nano-ribbons versus the bias that should be carefully considered for recognition.<\/jats:p>","DOI":"10.3390\/computation13020022","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T05:47:42Z","timestamp":1737438462000},"page":"22","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Operational Robustness of Amino Acid Recognition via Transverse Tunnelling Current Across Metallic Graphene Nano-Ribbon Electrodes: The Pro-Ser Case"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6082-8844","authenticated-orcid":false,"given":"Giuseppe","family":"Zollo","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze di Base e Applicate per l\u2019Ingegneria, Universit\u00e0 di Roma \u201cLa Sapienza\u201d, Via A. Scarpa 14-16, 00161 Rome, Italy"},{"name":"Infrastructure for Energy Transition and Circular Economy @ EuroNanoLab\u2014iENTRANCE@ENL, 00161 Rome, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1038\/nmeth.3290","article-title":"Improved Data Analysis for The MinION Nanopore Sequencer","volume":"12","author":"Jain","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/nbt.2181","article-title":"DNA sequencing with nanopores","volume":"30","author":"Schneider","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1038\/nnano.2016.120","article-title":"Reading the primary structure of a protein with 0.07 nm3 resolution using a subnanometre-diameter pore","volume":"11","author":"Kennedy","year":"2016","journal-title":"Nat. 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