{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T22:48:21Z","timestamp":1774046901062,"version":"3.50.1"},"reference-count":152,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T00:00:00Z","timestamp":1626998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002946","name":"Deutsches Zentrum f\u00fcr Luft- und Raumfahrt","doi-asserted-by":"publisher","award":["BMWi projects 50WB1524 and 50WB1924"],"award-info":[{"award-number":["BMWi projects 50WB1524 and 50WB1924"]}],"id":[{"id":"10.13039\/501100002946","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002946","name":"Deutsches Zentrum f\u00fcr Luft- und Raumfahrt","doi-asserted-by":"publisher","award":["BMWi project  50WB1524 and 50WB1924"],"award-info":[{"award-number":["BMWi project  50WB1524 and 50WB1924"]}],"id":[{"id":"10.13039\/501100002946","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computation"],"abstract":"<jats:p>When culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search of a target where the alteration of gravity force could have this impact, we investigated p130cas\/BCAR1 and its interactions more thoroughly, particularly as its activity is sensitive to applied forces. This protein is well characterized regarding its role in growth stimulation and adhesion processes. To better understand BCAR1\u2032s force-dependent scaffolding of other proteins, we studied its interactions with proteins we had detected by proteome analyses of MCF-7 breast cancer and FTC-133 thyroid cancer cells, which are both sensitive to exposure to microgravity and express BCAR1. Using linked open data resources and our experiments, we collected comprehensive information to establish a semantic knowledgebase and analyzed identified proteins belonging to signaling pathways and their networks. The results show that the force-dependent phosphorylation and scaffolding of BCAR1 influence the structure, function, and degradation of intracellular proteins as well as the growth, adhesion and apoptosis of cells similarly to exposure of whole cells to altered gravity. As BCAR1 evidently plays a significant role in cell responses to gravity changes, this study reveals a clear path to future research performing phosphorylation experiments on BCAR1.<\/jats:p>","DOI":"10.3390\/computation9080081","type":"journal-article","created":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T10:31:44Z","timestamp":1627036304000},"page":"81","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity"],"prefix":"10.3390","volume":"9","author":[{"given":"Johann","family":"Bauer","sequence":"first","affiliation":[{"name":"SiHaTho GmbH, Postfach 1106, 35201 Biedenkopf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2921-4454","authenticated-orcid":false,"given":"Erich","family":"Gombocz","sequence":"additional","affiliation":[{"name":"Melissa Informatics, 22382 Avenida Empresa, Rancho Sana Margarita, CA 92688, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert","family":"Schulz","sequence":"additional","affiliation":[{"name":"Department of Microgravity and Translational Regenerative Medicine, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University, 39106 Magdeburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2184-7000","authenticated-orcid":false,"given":"Jens","family":"Hauslage","sequence":"additional","affiliation":[{"name":"DLR, Institut f\u00fcr Luft- und Raumfahrtmedizin, Linder H\u00f6he, 51147 K\u00f6ln, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4991-3105","authenticated-orcid":false,"given":"Daniela","family":"Grimm","sequence":"additional","affiliation":[{"name":"Department of Microgravity and Translational Regenerative Medicine, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University, 39106 Magdeburg, Germany"},{"name":"Department of Biomedicine, Aarhus University, Ole Worms All\u00e9 4, DK-8000 Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1080\/14789450.2019.1550362","article-title":"Current knowledge about the impact of microgravity on the proteome","volume":"16","author":"Strauch","year":"2019","journal-title":"Expert Rev. 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