{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:43:27Z","timestamp":1760240607073,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,7,28]],"date-time":"2019-07-28T00:00:00Z","timestamp":1564272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R15GM128166-01"],"award-info":[{"award-number":["1R15GM128166-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Data"],"abstract":"<jats:p>Total internal reflection fluorescence (TIRF) microscope image sequences are commonly used to study receptors in live cells. The dataset presented herein facilitates the study of the IgE-Fc\u03b5RI receptor signaling complex (IgE-RC) in rat basophilic leukemia (RBL-2H3) cells coming into contact with a supported lipid bilayer with 25 mol% N-dinitrophenyl-aminocaproyl phosphatidylethanolamine, modeling an immunological synapse. TIRF microscopy was used to image IgE-RCs within this Fc\u03b5RI-centric synapse by loading RBL-2H3 cells with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE) in suspension for 24 h. Fluorescent anti-DNP IgE (IgE488) concentrations of this suspension increased from 10% to 100% and corresponding non-fluorescent anti-DNP IgE concentrations decreased from 90% to 0%. After the removal of unbound anti-DNP IgE, multiple image sequences were taken for each of these ten conditions. Prior to imaging, anti-DNP IgE-primed RBL-2H3 cells were either kept for a few minutes, for about 30 min, or for about one hour in Hanks buffer. The dataset contains 482 RBL-2H3 model synapse image stacks, dark images to correct for background intensity, and TIRF illumination profile images to correct for non-uniform TIRF illumination. After background subtraction, non-uniform illumination correction, and conversion of pixel units from analog-to-digital units to photo electrons, the average pixel intensity was calculated. The average pixel intensity within Fc\u03b5RI-centric synapses for all three Hanks buffer conditions increased linearly at a rate of 0.42 \u00b1 0.02 photo electrons per pixel per % IgE488 in suspension. RBL-2H3 cell degranulation was tested by detecting \u03b2-hexosaminidase activity. Prolonged RBL-2H3 cell exposure to Hanks buffer inhibited exocytosis in RBL-2H3 cells.<\/jats:p>","DOI":"10.3390\/data4030111","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T03:06:58Z","timestamp":1564369618000},"page":"111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["TIRF Microscope Image Sequences of Fluorescent IgE-Fc\u03b5RI Receptor Complexes inside a Fc\u03b5RI-Centric Synapse in RBL-2H3 Cells"],"prefix":"10.3390","volume":"4","author":[{"given":"Rachel","family":"Drawbond","sequence":"first","affiliation":[{"name":"UCCS Center of the Biofrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"},{"name":"Department of Mathematics, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5784-5253","authenticated-orcid":false,"given":"Kathrin","family":"Spendier","sequence":"additional","affiliation":[{"name":"Department of Mathematics, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"},{"name":"Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.copbio.2010.08.006","article-title":"Quantitative understanding of cell signaling: The importance of membrane organization","volume":"21","author":"Radhakrishnan","year":"2010","journal-title":"Curr. 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