{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T05:38:15Z","timestamp":1775021895726,"version":"3.50.1"},"reference-count":13,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["304870\/2013-7, 445207\/2014-0, 201285\/2015-0"],"award-info":[{"award-number":["304870\/2013-7, 445207\/2014-0, 201285\/2015-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad Santiago de Cali","award":["934-621119-561"],"award-info":[{"award-number":["934-621119-561"]}]},{"name":"Ministerio de Ciencia, Tecnologia e Innovacio\u0301n MINCIENCIAS","award":["512, 784"],"award-info":[{"award-number":["512, 784"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Data"],"abstract":"<jats:p>We used Nile red to estimate lipid content in oleaginous yeasts using a high-throughput approach. We measured the fluorescence intensity of Nile red using different solvents, yeast strains, and incubation times in optimized excitation\/emission wavelengths. The data show the relative fluorescence units (RFU) for Nile red excitation, using 1\u00d7 PBS, 1\u00d7 PBS and 5% v\/v isopropyl alcohol, 50% v\/v glycerol, culture medium A-gly broth, and A-gly broth supplemented with 5% v\/v DMSO. In addition, we showed the RFU for the Nile red dye for different oleaginous and non-oleaginous yeast strains, such as Meyerozyma guilliermondii BI281A, Yarrowia lipolytica QU21 and Saccharomyces cerevisiae MRC164. Other measurements of lipid accumulation kinetics were shown for the above and additional yeast strains. These datasets provide the guidelines to obtain the optimal solvent system and the minimal interaction time for the Nile red dye to enter in the cells and obtain a stable readout.<\/jats:p>","DOI":"10.3390\/data5030077","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T08:53:43Z","timestamp":1598950423000},"page":"77","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dataset of Nile Red Fluorescence Readings with Different Yeast Strains, Solvents, and Incubation Times"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8525-5341","authenticated-orcid":false,"given":"Mauricio","family":"Ramirez-Castrillon","sequence":"first","affiliation":[{"name":"Research Group in Mycology (GIM\/CICBA), Universidad Santiago de Cali, Calle 5 No. 62-00, Santiago de Cali, Colombia"},{"name":"Graduate Program in Cell and Molecular Biology, Biotechnology Center, Universidade Federal do Rio Grande do Sul, Av. Bento Gon\u00e7alves, 9500 Pr\u00e9dios 43421\/43431 - Setor IV - Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"Jaramillo-Garcia","sequence":"additional","affiliation":[{"name":"Graduate Program in Cell and Molecular Biology, Biotechnology Center, Universidade Federal do Rio Grande do Sul, Av. Bento Gon\u00e7alves, 9500 Pr\u00e9dios 43421\/43431 - Setor IV - Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helio","family":"Barros","sequence":"additional","affiliation":[{"name":"New Organic Materials and Forensic Chemistry Laboratory (LNMO-QF), Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gon\u00e7alves, 9500, Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5298-932X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Henriques","sequence":"additional","affiliation":[{"name":"Graduate Program in Cell and Molecular Biology, Biotechnology Center, Universidade Federal do Rio Grande do Sul, Av. Bento Gon\u00e7alves, 9500 Pr\u00e9dios 43421\/43431 - Setor IV - Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valter","family":"Stefani","sequence":"additional","affiliation":[{"name":"New Organic Materials and Forensic Chemistry Laboratory (LNMO-QF), Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gon\u00e7alves, 9500, Campus do Vale - CxP. 15005, Porto Alegre, RS CEP 91501-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Valente","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS CEP 90050-170, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.mimet.2003.10.018","article-title":"Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence","volume":"56","author":"Kimura","year":"2004","journal-title":"J. Microbiol. Methods."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1016\/j.biotechadv.2014.08.003","article-title":"Oleaginous yeasts for biodiesel: Current and future trends in biology and production","volume":"32","author":"Sitepu","year":"2014","journal-title":"Biotechnol. Adv."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Rostron, K.A., and Lawrence, C.L. (2017). Nile red staining of neutral lipids in yeast. Histochemistry of Single Molecules, Humana Press.","DOI":"10.1007\/978-1-4939-6788-9_16"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1016\/j.renene.2018.12.078","article-title":"Innovative screening approach for the identification of triacylglycerol accumulating oleaginous strains","volume":"135","author":"Patel","year":"2019","journal-title":"Renew. Energy"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Miranda, C., Bettencourt, S., Pozdniakova, T., Pereira, J., Sampaio, P., Franco-Duarte, R., and Pais, C. (2020). Modified high-throughput Nile red fluorescence assay for the rapid screening of oleaginous yeasts using acetic acid as carbon source. BMC Microbiol., 20.","DOI":"10.1186\/s12866-020-01742-6"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Balan, V. (2019). Microbial Lipid Production, Humana Press.","DOI":"10.1007\/978-1-4939-9484-7"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1080\/10826068.2019.1587624","article-title":"Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method","volume":"49","author":"Zhao","year":"2019","journal-title":"Prep. Biochem. 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PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0105870"},{"key":"ref_11","first-page":"144","article-title":"Biodiversity of yeasts associated to bromeliads in Itapu\u00e3 park, Viam\u00e3o\/RS","volume":"14","author":"Landell","year":"2006","journal-title":"Bioci\u00eancias"},{"key":"ref_12","unstructured":"Gomes, F.F. (2016). Identifica\u00e7\u00e3o molecular da comunidade de leveduras associadas a macr\u00f3fitas aqu\u00e1ticas em uma regi\u00e3o de marisma da Lagoa dos Patos (RS). [Bachelor\u2019s Thesis, Universidade Federal do Rio Grande do Sul]."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ramirez-Castrillon, M., Gomes, F.F., Souza, A.F., Leite, B.R., Pagani, D.M., and Valente, P. (2020). Papiliotrema maritimi f.a. sp. nov., a new tremellaceous yeast species associated to macrophytes in a Marshland of South Brazil. bioRxiv.","DOI":"10.1101\/2020.06.15.153189"}],"container-title":["Data"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5729\/5\/3\/77\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:05:21Z","timestamp":1760177121000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5729\/5\/3\/77"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,1]]},"references-count":13,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["data5030077"],"URL":"https:\/\/doi.org\/10.3390\/data5030077","relation":{},"ISSN":["2306-5729"],"issn-type":[{"value":"2306-5729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,1]]}}}