{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T14:31:09Z","timestamp":1778509869500,"version":"3.51.4"},"reference-count":25,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T00:00:00Z","timestamp":1653868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH CTSI Miami","award":["Pilot-FY19-05"],"award-info":[{"award-number":["Pilot-FY19-05"]}]},{"name":"NIH CTSI Miami","award":["1R01CA215973"],"award-info":[{"award-number":["1R01CA215973"]}]},{"name":"NIH CTSI Miami","award":["P20GM103643"],"award-info":[{"award-number":["P20GM103643"]}]},{"DOI":"10.13039\/100000054","name":"NIH\/NCI","doi-asserted-by":"publisher","award":["Pilot-FY19-05"],"award-info":[{"award-number":["Pilot-FY19-05"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"NIH\/NCI","doi-asserted-by":"publisher","award":["1R01CA215973"],"award-info":[{"award-number":["1R01CA215973"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"NIH\/NCI","doi-asserted-by":"publisher","award":["P20GM103643"],"award-info":[{"award-number":["P20GM103643"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIGMS","award":["Pilot-FY19-05"],"award-info":[{"award-number":["Pilot-FY19-05"]}]},{"name":"NIGMS","award":["1R01CA215973"],"award-info":[{"award-number":["1R01CA215973"]}]},{"name":"NIGMS","award":["P20GM103643"],"award-info":[{"award-number":["P20GM103643"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Data"],"abstract":"<jats:p>Paclitaxel-induced peripheral neuropathy is a condition of nerve degeneration induced by chemotherapy, which afflicts up to 70% of treated patients. Therapeutic interventions are unavailable due to an incomplete understanding of the underlying mechanisms. We previously discovered that major physiological changes in the skin underlie paclitaxel-induced peripheral neuropathy in zebrafish and rodents. The precise molecular mechanisms are only incompletely understood. For instance, paclitaxel induces the upregulation of MMP-13, which, when inhibited, prevents axon degeneration. To better understand other gene regulatory changes induced by paclitaxel, we induced peripheral neuropathy in mice following intraperitoneal injection either with vehicle or paclitaxel every other day four times total. Skin and dorsal root ganglion neurons were collected based on distinct behavioural responses categorised as \u201cpain onset\u201d (d4), \u201cmaximal pain\u201d (d7), \u201cbeginning of pain resolution\u201d (d11), and \u201crecovery phase\u201d (d23) for comparative longitudinal RNA sequencing. The generated datasets validate previous discoveries and reveal additional gene expression changes that warrant further validation with the goal to aid in the development of drugs that prevent or reverse paclitaxel-induced peripheral neuropathy.<\/jats:p>","DOI":"10.3390\/data7060072","type":"journal-article","created":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T10:05:14Z","timestamp":1653905114000},"page":"72","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Longitudinal RNA Sequencing of Skin and DRG Neurons in Mice with Paclitaxel-Induced Peripheral Neuropathy"],"prefix":"10.3390","volume":"7","author":[{"given":"Anthony M.","family":"Cirrincione","sequence":"first","affiliation":[{"name":"Department of Biology, University of Miami, Coral Gables, FL 33146, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassandra A.","family":"Reimonn","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of New England, Biddeford, ME 04005, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin J.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of New England, Biddeford, ME 04005, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9059-1670","authenticated-orcid":false,"given":"Sandra","family":"Rieger","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Miami, Coral Gables, FL 33146, USA"},{"name":"Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.2217\/pmt.15.19","article-title":"Mechanisms involved in the development of chemotherapy-induced neuropathy","volume":"5","author":"Walters","year":"2015","journal-title":"Pain Manag."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1093\/bja\/aex229","article-title":"Clinical and preclinical perspectives on Chemotherapy-Induced Peripheral Neuropathy (CIPN): A narrative review","volume":"119","author":"Flatters","year":"2017","journal-title":"Br. J. Anaesth."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.neuropharm.2013.08.016","article-title":"The paradox of paclitaxel neurotoxicity: Mechanisms and unanswered questions","volume":"76 Pt A","author":"Gornstein","year":"2014","journal-title":"Neuropharmacology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.pain.2010.10.030","article-title":"Intraepidermal nerve fiber loss corresponds to the development of taxol-induced hyperalgesia and can be prevented by treatment with minocycline","volume":"152","author":"Xin","year":"2011","journal-title":"Pain"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1111\/j.1460-9568.2011.07652.x","article-title":"Terminal arbor degeneration\u2014A novel lesion produced by the antineoplastic agent paclitaxel","volume":"33","author":"Bennett","year":"2011","journal-title":"Eur. J. Neurosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.expneurol.2007.11.001","article-title":"Prevention of paclitaxel-evoked painful peripheral neuropathy by acetyl-L-carnitine: Effects on axonal mitochondria, sensory nerve fiber terminal arbors, and cutaneous Langerhans cells","volume":"210","author":"Jin","year":"2008","journal-title":"Exp. Neurol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.npep.2014.02.001","article-title":"Peptidergic intraepidermal nerve fibers in the skin contribute to the neuropathic pain in paclitaxel-induced peripheral neuropathy","volume":"48","author":"Ko","year":"2014","journal-title":"Neuropeptides"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1073\/pnas.1525096113","article-title":"Paclitaxel-induced epithelial damage and ectopic MMP-13 expression promotes neurotoxicity in zebrafish","volume":"113","author":"Lisse","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jpain.2019.06.008","article-title":"Inflammatory and Neuropathic Gene Expression Signatures of Chemotherapy-Induced Neuropathy Induced by Vincristine, Cisplatin, and Oxaliplatin in C57BL\/6J Mice","volume":"21","author":"Starobova","year":"2020","journal-title":"J. Pain"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3970","DOI":"10.1038\/s41598-020-60990-8","article-title":"Paclitaxel-induced peripheral neuropathy is caused by epidermal ROS and mitochondrial damage through conserved MMP-13 activation","volume":"10","author":"Cirrincione","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1097\/PR9.0000000000000785","article-title":"Spinal cord stimulation prevents paclitaxel-induced mechanical and cold hypersensitivity and modulates spinal gene expression in rats","volume":"4","author":"Sivanesan","year":"2019","journal-title":"Pain Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s12974-021-02098-y","article-title":"Transcriptome profiling of long noncoding RNAs and mRNAs in spinal cord of a rat model of paclitaxel-induced peripheral neuropathy identifies potential mechanisms mediating neuroinflammation and pain","volume":"18","author":"Li","year":"2021","journal-title":"J. Neuroinflammation"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1744806918816462","DOI":"10.1177\/1744806918816462","article-title":"Expression of mitochondrial dysfunction-related genes and pathways in paclitaxel-induced peripheral neuropathy in breast cancer survivors","volume":"14","author":"Kober","year":"2018","journal-title":"Mol. Pain"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wang, Y., Shan, Q., Meng, Y., Pan, J., and Yi, S. (2017). Mrpl10 and Tbp Are Suitable Reference Genes for Peripheral Nerve Crush Injury. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18020263"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.jpain.2016.02.011","article-title":"Dorsal Root Ganglion Infiltration by Macrophages Contributes to Paclitaxel Chemotherapy-Induced Peripheral Neuropathy","volume":"17","author":"Zhang","year":"2016","journal-title":"J. Pain"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2006050118","DOI":"10.1073\/pnas.2006050118","article-title":"Integrins protect sensory neurons in models of paclitaxel-induced peripheral sensory neuropathy","volume":"118","author":"Shin","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1038\/jid.2008.247","article-title":"Candidate housekeeping genes require evaluation before their selection for studies of human epidermal keratinocytes","volume":"129","author":"Minner","year":"2009","journal-title":"J. Investig. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1111\/j.1600-0625.2012.01537.x","article-title":"Identification and validation of reference genes for expression studies in human keratinocyte cell lines treated with and without interferon-\u03b3\u2014A method for qRT-PCR reference gene determination","volume":"21","author":"Riemer","year":"2012","journal-title":"Exp. Derm."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"12067","DOI":"10.1038\/s41598-020-69008-9","article-title":"TIMP-3 facilitates binding of target metalloproteinases to the endocytic receptor LRP-1 and promotes scavenging of MMP-1","volume":"10","author":"Carreca","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1002\/emmm.201202100","article-title":"Matrix metalloproteinase 13 modulates intestinal epithelial barrier integrity in inflammatory diseases by activating TNF","volume":"5","author":"Vandenbroucke","year":"2013","journal-title":"EMBO Mol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"01001","DOI":"10.1128\/JVI.01001-17","article-title":"RNA-Seq Analysis of Differentiated Keratinocytes Reveals a Massive Response to Late Events during Human Papillomavirus 16 Infection, Including Loss of Epithelial Barrier Function","volume":"91","author":"Klymenko","year":"2017","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1146\/annurev.pa.20.040180.002301","article-title":"Efficient analysis of experimental observations","volume":"20","author":"Dixon","year":"1980","journal-title":"Annu. Rev. Pharm. Toxicol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0165-0270(94)90144-9","article-title":"Quantitative assessment of tactile allodynia in the rat paw","volume":"53","author":"Chaplan","year":"1994","journal-title":"J. Neurosci. Methods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"}],"container-title":["Data"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5729\/7\/6\/72\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:22:02Z","timestamp":1760138522000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5729\/7\/6\/72"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,30]]},"references-count":25,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["data7060072"],"URL":"https:\/\/doi.org\/10.3390\/data7060072","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-960602\/v1","asserted-by":"object"}]},"ISSN":["2306-5729"],"issn-type":[{"value":"2306-5729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,30]]}}}