{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T21:56:59Z","timestamp":1776722219129,"version":"3.51.2"},"reference-count":35,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T00:00:00Z","timestamp":1698969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["075-15-2021-1055"],"award-info":[{"award-number":["075-15-2021-1055"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Data"],"abstract":"<jats:p>Lysinibacillus sphaericus holds a significant agricultural importance by being able to produce insecticidal toxins and chemical moieties of varying antibacterial and fungicidal activities. In this study, the genome of the L. sphaericus strain 1795 is presented. Illumina short reads sequenced on the HiSeq X platform were used to obtain the genome\u2019s assembly by applying the SPAdes v3.15.4 software. The genome size based on a cumulative length of 23 contigs reached 4.74 Mb, with a respective N50 of 1.34 Mb. The assembled genome carried 4672 genes, including 4643 protein-encoding ones, 5 of which represented loci coding for insecticidal toxins active against the orders Diptera, Lepidoptera, and Blattodea. We also revealed biosynthetic gene clusters responsible for the synthesis of secondary metabolites with predicted antibacterial, fungicidal, and growth-promoting properties. The genomic data provided will be helpful for deepening our understanding of genetic markers determining the efficient application of the L. sphaericus strain 1795 primarily for biocontrol purposes in veterinary and medical applications against several groups of blood-sucking insects.<\/jats:p>","DOI":"10.3390\/data8110167","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T10:51:28Z","timestamp":1699008688000},"page":"167","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Draft Genome Sequence Data of Lysinibacillus sphaericus Strain 1795 with Insecticidal Properties"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3990-4336","authenticated-orcid":false,"given":"Maria N.","family":"Romanenko","sequence":"first","affiliation":[{"name":"All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia"},{"name":"Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia"}]},{"given":"Maksim A.","family":"Nesterenko","sequence":"additional","affiliation":[{"name":"All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7084-0177","authenticated-orcid":false,"given":"Anton E.","family":"Shikov","sequence":"additional","affiliation":[{"name":"All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia"},{"name":"Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8338-3494","authenticated-orcid":false,"given":"Anton A.","family":"Nizhnikov","sequence":"additional","affiliation":[{"name":"All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia"},{"name":"Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8575-2601","authenticated-orcid":false,"given":"Kirill S.","family":"Antonets","sequence":"additional","affiliation":[{"name":"All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia"},{"name":"Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jip.2011.11.008","article-title":"The Bacterium, Lysinibacillus sphaericus, as an Insect Pathogen","volume":"109","author":"Berry","year":"2012","journal-title":"J. Invertebr. Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/0022-2011(65)90120-5","article-title":"Bacillus sphaericus Neide as a Pathogen of Mosquitoes","volume":"7","author":"Kellen","year":"1965","journal-title":"J. Invertebr. Pathol."},{"key":"ref_3","unstructured":"Silva Filha, M.H.N.L., Berry, C., and Regis, L. (2014). Advances in Insect Physiology, Elsevier."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1111\/j.1365-2672.1992.tb01847.x","article-title":"Biological Control of Mosquitoes and Other Biting Flies by Bacillus sphaericus and Bacillus thuringiensis","volume":"72","author":"Priest","year":"1992","journal-title":"J. Appl. Bacteriol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.en.41.010196.002315","article-title":"Bacillus sphaericus Toxins: Molecular Biology and Mode of Action","volume":"41","author":"Charles","year":"1996","journal-title":"Annu. Rev. Entomol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Onajobi, I.B., Idowu, E.O., Adeyemi, J.O., Samson, O.J., Ogunyinka, P.I., and Fagade, O.E. (2020). In Vitro Antibacterial Activities and Molecular Characterization of Bacterial Species Isolated from Farmlands against Selected Pathogens. Biotechnol. Rep., 27.","DOI":"10.1016\/j.btre.2020.e00513"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/j.1432-1033.1995.tb20251.x","article-title":"Resistance in a Laboratory Population of Culex quinquefasciatus (Diptera: Culicidae) to Bacillus sphaericus Binary Toxin Is Due to a Change in the Receptor on Midgut Brush-Border Membranes","volume":"228","author":"Charles","year":"1995","journal-title":"Eur. J. Biochem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jip.2009.03.005","article-title":"Purification and Characterization of Mosquitocidal Bacillus sphaericus BinA Protein","volume":"101","author":"Hire","year":"2009","journal-title":"J. Invertebr. Pathol."},{"key":"ref_9","unstructured":"Glare, T.R., Jurat-Fuentes, J.-L., and O\u2019Callaghan, M. (2017). Microbial Control of Insect and Mite Pests, Elsevier."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1603\/0022-2585-37.4.534","article-title":"Laboratory Selection for Resistance to Bacillus sphaericus in Culex quinquefasciatus (Diptera: Culicidae) from California, USA","volume":"37","author":"Wirth","year":"2000","journal-title":"J. Med. Entomol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1007\/s11274-013-1301-9","article-title":"Metal Tolerance and Larvicidal Activity of Lysinibacillus sphaericus","volume":"29","author":"Lozano","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1128\/jb.62.3.293-300.1951","article-title":"Studies on lysogenesis I","volume":"62","author":"Bertani","year":"1951","journal-title":"J. Bacteriol."},{"key":"ref_13","unstructured":"Andrews, S. (2023, May 04). FastQC: A Quality Control Tool for High Throughput Sequence Data. Available online: https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra-Fast All-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: Assessing the Quality of Microbial Genomes Recovered from Isolates, Single Cells, and Metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: Quality Assessment Tool for Genome Assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4647","DOI":"10.1093\/molbev\/msab199","article-title":"BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes","volume":"38","author":"Manni","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"D733","DOI":"10.1093\/nar\/gkv1189","article-title":"Reference Sequence (RefSeq) Database at NCBI: Current Status, Taxonomic Expansion, and Functional Annotation","volume":"44","author":"Wright","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","article-title":"High Throughput ANI Analysis of 90K Prokaryotic Genomes Reveals Clear Species Boundaries","volume":"9","author":"Jain","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid Prokaryotic Genome Annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1093\/bioinformatics\/btab506","article-title":"BtToxin_Digger: A Comprehensive and High-Throughput Pipeline for Mining Toxin Protein Genes from Bacillus thuringiensis","volume":"38","author":"Liu","year":"2021","journal-title":"Bioinformatics"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Shikov, A.E., Malovichko, Y.V., Skitchenko, R.K., Nizhnikov, A.A., and Antonets, K.S. (2020). No More Tears: Mining Sequencing Data for Novel Bt Cry Toxins with CryProcessor. Toxins, 12.","DOI":"10.3390\/toxins12030204"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"baac022","DOI":"10.1093\/database\/baac022","article-title":"BPPRC Database: A Web-Based Tool to Access and Analyse Bacterial Pesticidal Proteins","volume":"2022","author":"Panneerselvam","year":"2022","journal-title":"Database"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e110","DOI":"10.1093\/nar\/gkz654","article-title":"A Deep Learning Genome-Mining Strategy for Biosynthetic Gene Cluster Prediction","volume":"47","author":"Hannigan","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"W29","DOI":"10.1093\/nar\/gkab335","article-title":"AntiSMASH 6.0: Improving Cluster Detection and Comparison Capabilities","volume":"49","author":"Blin","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1590\/S1415-47572012000600020","article-title":"Plant Growth-Promoting Rhizobacteria (PGPR): Their Potential as Antagonists and Biocontrol Agents","volume":"35","author":"Beneduzi","year":"2012","journal-title":"Genet. Mol. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"888","DOI":"10.7164\/antibiotics.39.888","article-title":"Fengycin\u2014A Novel Antifungal Lipopeptide Antibiotic Produced by Bacillus subtilis F-29-3","volume":"39","author":"Vanittanakom","year":"1986","journal-title":"J. Antibiot."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.tim.2007.12.009","article-title":"Bacillus Lipopeptides: Versatile Weapons for Plant Disease Biocontrol","volume":"16","author":"Ongena","year":"2008","journal-title":"Trends Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.1007\/s11356-015-4294-0","article-title":"Microbial Siderophores and Their Potential Applications: A Review","volume":"23","author":"Saha","year":"2016","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Saleem, F., and Shakoori, A. (2017). The First Cry2Ac-Type Protein Toxic to Helicoverpa armigera: Cloning and Overexpression of cry2ac7 Gene from SBS-BT1 Strain of Bacillus thuringiensis. Toxins, 9.","DOI":"10.3390\/toxins9110358"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1073\/pnas.44.10.1072","article-title":"Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate","volume":"44","author":"Spizizen","year":"1958","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"D807","DOI":"10.1093\/nar\/gky1053","article-title":"OrthoDB V10: Sampling the Diversity of Animal, Plant, Fungal, Protist, Bacterial and Viral Genomes for Evolutionary and Functional Annotations of Orthologs","volume":"47","author":"Kriventseva","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Hyatt, D., Chen, G.-L., LoCascio, P.F., Land, M.L., Larimer, F.W., and Hauser, L.J. (2010). Prodigal: Prokaryotic Gene Recognition and Translation Initiation Site Identification. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-119"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"D8","DOI":"10.1093\/nar\/gkx1095","article-title":"Database Resources of the National Center for Biotechnology Information","volume":"46","author":"Agarwala","year":"2018","journal-title":"Nucleic Acids Res."}],"container-title":["Data"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5729\/8\/11\/167\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:16:39Z","timestamp":1760130999000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5729\/8\/11\/167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,3]]},"references-count":35,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["data8110167"],"URL":"https:\/\/doi.org\/10.3390\/data8110167","relation":{},"ISSN":["2306-5729"],"issn-type":[{"value":"2306-5729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,3]]}}}