{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:21:52Z","timestamp":1760145712460,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,25]],"date-time":"2024-08-25T00:00:00Z","timestamp":1724544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Augustinus Foundation","award":["19-2384"],"award-info":[{"award-number":["19-2384"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Imaging"],"abstract":"<jats:p>The aim was to establish combined H215O PET\/MRI during ex vivo normothermic machine perfusion (NMP) of isolated porcine kidneys. We examined whether changes in renal arterial blood flow (RABF) are accompanied by changes of a similar magnitude in renal blood perfusion (RBP) as well as the relation between RBP and renal parenchymal oxygenation (RPO). Methods: Pig kidneys (n = 7) were connected to a NMP circuit. PET\/MRI was performed at two different pump flow levels: a blood-oxygenation-level-dependent (BOLD) MRI sequence performed simultaneously with a H215O PET sequence for determination of RBP. Results: RBP was measured using H215O PET in all kidneys (flow 1: 0.42\u20130.76 mL\/min\/g, flow 2: 0.7\u20131.6 mL\/min\/g). We found a linear correlation between changes in delivered blood flow from the perfusion pump and changes in the measured RBP using PET imaging (r2 = 0.87). Conclusion: Our study demonstrated the feasibility of combined H215O PET\/MRI during NMP of isolated porcine kidneys with tissue oxygenation being stable over time. The introduction of H215O PET\/MRI in nephrological research could be highly relevant for future pre-transplant kidney evaluation and as a tool for studying renal physiology in healthy and diseased kidneys.<\/jats:p>","DOI":"10.3390\/jimaging10090209","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T01:48:49Z","timestamp":1724636929000},"page":"209","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ex Vivo Simultaneous H215O Positron Emission Tomography and Magnetic Resonance Imaging of Porcine Kidneys\u2014A Feasibility Study"],"prefix":"10.3390","volume":"10","author":[{"given":"Maibritt Meldgaard","family":"Arildsen","sequence":"first","affiliation":[{"name":"Comparative Medicine Lab, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 11, 8200 Aarhus, Denmark"}]},{"given":"Christian \u00d8stergaard","family":"Mariager","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine and PET Centre, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus, Denmark"}]},{"given":"Christoffer Vase","family":"Overgaard","sequence":"additional","affiliation":[{"name":"Comparative Medicine Lab, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 11, 8200 Aarhus, Denmark"}]},{"given":"Thomas","family":"Vorre","sequence":"additional","affiliation":[{"name":"Comparative Medicine Lab, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 11, 8200 Aarhus, Denmark"}]},{"given":"Martin","family":"B\u00f8jesen","sequence":"additional","affiliation":[{"name":"Comparative Medicine Lab, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 11, 8200 Aarhus, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0827-5263","authenticated-orcid":false,"given":"Niels","family":"Moeslund","sequence":"additional","affiliation":[{"name":"Comparative Medicine Lab, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 11, 8200 Aarhus, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0084-9122","authenticated-orcid":false,"given":"Aage Kristian Olsen","family":"Alstrup","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine and PET Centre, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus, Denmark"},{"name":"Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 99, 8200 Aarhus, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8105-7046","authenticated-orcid":false,"given":"Lars Poulsen","family":"Tolbod","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine and PET Centre, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus, Denmark"}]},{"given":"Mikkel Holm","family":"Vendelbo","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine and PET Centre, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus, Denmark"},{"name":"Department of Biomedicine, Aarhus University, H\u00f8egh-Guldbergs Gade 10, 8000 Aarhus, Denmark"}]},{"given":"Steffen","family":"Ringgaard","sequence":"additional","affiliation":[{"name":"MR Centre, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 99, 8200 Aarhus, Denmark"}]},{"given":"Michael","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Comparative Medicine Lab, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 11, 8200 Aarhus, Denmark"},{"name":"Radiology Research Unit, South Danish University, Kl\u00f8verv\u00e6nget 10, 5000 Odense, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7972-8181","authenticated-orcid":false,"given":"Niels Henrik","family":"Buus","sequence":"additional","affiliation":[{"name":"Department of Renal Medicine, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus, Denmark"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1016\/S0140-6736(16)32064-5","article-title":"Chronic Kidney Disease","volume":"389","author":"Webster","year":"2017","journal-title":"Lancet"},{"key":"ref_2","unstructured":"Vaidya, S.R., and Aeddula, N.R. 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