{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T16:46:54Z","timestamp":1776530814456,"version":"3.51.2"},"reference-count":24,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,1,22]],"date-time":"2019-01-22T00:00:00Z","timestamp":1548115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002803","name":"Fondazione Cariplo","doi-asserted-by":"publisher","award":["2012-0685"],"award-info":[{"award-number":["2012-0685"]}],"id":[{"id":"10.13039\/501100002803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Imaging"],"abstract":"<jats:p>The balance of lung extravascular water depends upon the control of blood flow in the alveolar distribution vessels that feed downstream two districts placed in parallel, the corner vessels and the alveolar septal network. The occurrence of an edemagenic condition appears critical as an increase in extravascular water endangers the thinness of the air\u2013blood barrier, thus negatively affecting the diffusive capacity of the lung. We exposed anesthetized rabbits to an edemagenic factor (12% hypoxia) for 120 min and followed by in vivo imaging the micro-vascular morphology through a \u201cpleural window\u201d using a stereo microscope at a magnification of 15\u00d7 (resolution of 7.2 \u03bcm). We measured the change in diameter of distribution vessels (50\u2013200 \u03bcm) and corner vessels (&lt;50 \u03bcm). On average, hypoxia caused a significant decrease in diameter of both smaller distribution vessels (about ~50%) and corner vessels (about ~25%) at 30 min. After 120 min, reperfusion occurred. Regional differences in perivascular interstitial volume were observed and could be correlated with differences in blood flow control. To understand such difference, we modelled imaged alveolar capillary units, obtained by Voronoi method, integrating microvascular pressure parameters with capillary filtration. Results of the analysis suggested that at 120 min, alveolar blood flow was diverted to the corner vessels in larger alveoli, which were found also to undergo a greater filtration indicating greater proneness to develop lung edema.<\/jats:p>","DOI":"10.3390\/jimaging5020022","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T03:52:32Z","timestamp":1548301952000},"page":"22","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging"],"prefix":"10.3390","volume":"5","author":[{"given":"Enrico","family":"Mazzuca","sequence":"first","affiliation":[{"name":"Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2950-0231","authenticated-orcid":false,"given":"Andrea","family":"Aliverti","sequence":"additional","affiliation":[{"name":"Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Miserocchi","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Universit\u00e0 di Milano Bicocca, 20900 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1183\/09059180.00002709","article-title":"Mechanisms controlling the volume of pleural fluid and extravascular lung water","volume":"18","author":"Miserocchi","year":"2009","journal-title":"Eur. Respir. Rev. Off. J. Eur. Respir. Soc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"L235","DOI":"10.1152\/ajplung.00064.2008","article-title":"Physiological determinants of the pulmonary filtration coefficient","volume":"295","author":"Parker","year":"2008","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"L881","DOI":"10.1152\/ajplung.2001.280.5.L881","article-title":"Pulmonary interstitial pressure and tissue matrix structure in acute hypoxia","volume":"280","author":"Miserocchi","year":"2001","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3109\/10739689509146765","article-title":"Pulmonary microvascular pressure profile during development of hydrostatic edema","volume":"2","author":"Negrini","year":"1995","journal-title":"Microcirculation"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1183\/09031936.00066710","article-title":"Interstitial pressure and lung oedema in chronic hypoxia","volume":"37","author":"Rivolta","year":"2011","journal-title":"Eur. Respir. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.resp.2014.07.012","article-title":"Alveolar mechanics studied by in vivo microscopy imaging through intact pleural space","volume":"202","author":"Salito","year":"2014","journal-title":"Respir. Physiol. Neurobiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jtbi.2016.03.036","article-title":"Computational micro-scale model of control of extravascular water and capillary perfusion in the air blood barrier","volume":"400","author":"Mazzuca","year":"2016","journal-title":"J. Theor. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"L1464","DOI":"10.1152\/ajplung.2001.281.6.L1464","article-title":"Pulmonary microvascular and perivascular interstitial geometry during development of mild hydraulic edema","volume":"281","author":"Negrini","year":"2001","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"455","DOI":"10.3109\/10739689709146809","article-title":"A semiautomatic procedure for edge detection of in vivo pulmonary microvessels and interstitial space","volume":"4","author":"Crisafulli","year":"1997","journal-title":"Microcirculation"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"L766","DOI":"10.1152\/ajplung.00313.2001","article-title":"Morpho-functional analysis of lung tissue in mild interstitial edema","volume":"282","author":"Conforti","year":"2002","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.resp.2011.06.024","article-title":"Morphometry of subpleural alveoli may be greatly biased by local pressure changes induced by the microscopic device","volume":"178","author":"Liu","year":"2011","journal-title":"Respir. Physiol. Neurobiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Schwenninger, D., Moller, K., Lui, H., Stahl, H.R.C., Schumann, S., and Guttmann, J. (2008, January 16\u201318). Determining Alveolar Dynamics by Automatic Tracing of Area Changes Within Microscopy Videos. Proceedings of the 2008 2nd International Conference on Bioinformatics and Biomedical Engineering, Shanghai, China.","DOI":"10.1109\/ICBBE.2008.916"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1152\/japplphysiol.00348.2007","article-title":"Intravital microscopy of the murine pulmonary microcirculation","volume":"104","author":"Tabuchi","year":"2008","journal-title":"J. Appl. Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1152\/japplphysiol.00733.2004","article-title":"Hypoxic pulmonary vasoconstriction","volume":"98","author":"Moudgil","year":"2005","journal-title":"J. Appl. Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1161\/01.RES.00000175934.68087.29","article-title":"Vascular remodeling versus vasoconstriction in chronic hypoxic pulmonary hypertension: A time for reappraisal?","volume":"97","author":"Stenmark","year":"2005","journal-title":"Circ. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1378\/chest.09-1355","article-title":"Exaggerated pulmonary hypertension during mild exercise in chronic mountain sickness","volume":"137","author":"Stuber","year":"2010","journal-title":"Chest"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"8381653","DOI":"10.1155\/2017\/8381653","article-title":"Thin Air Resulting in High Pressure: Mountain Sickness and Hypoxia-Induced Pulmonary Hypertension","volume":"2017","author":"Grimminger","year":"2017","journal-title":"Can. Respir. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1161\/01.CIR.101.12.1418","article-title":"Hypoxia-induced pulmonary blood redistribution in subjects with a history of high-altitude pulmonary edema","volume":"101","author":"Hanaoka","year":"2000","journal-title":"Circulation"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"82","DOI":"10.2174\/157339812799859789","article-title":"Mechanistic Considerations on the Development of Lung Edema: Vascular, Perivascular and Molecular Aspects from Early Stage to Tissue and Vascular Remodeling Stage","volume":"8","author":"Miserocchi","year":"2012","journal-title":"Curr. Respir. Med. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1054\/mehy.2001.1288","article-title":"Model of interstitial pressure as a result of cyclical changes in the capillary wall fluid transport","volume":"57","author":"Kurbel","year":"2001","journal-title":"Med. Hypotheses"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1152\/jappl.1979.47.2.383","article-title":"Capillary recruitment during airway hypoxia: Role of pulmonary artery pressure","volume":"47","author":"Wagner","year":"1979","journal-title":"J. Appl. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.resp.2011.04.005","article-title":"Pulmonary capillary recruitment in response to hypoxia in healthy humans: A possible role for hypoxic pulmonary venoconstriction?","volume":"177","author":"Taylor","year":"2011","journal-title":"Respir. Physiol. Neurobiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00221","DOI":"10.1002\/phy2.221","article-title":"From morphological heterogeneity at alveolar level to the overall mechanical lung behavior: An in vivo microscopic imaging study","volume":"2","author":"Mazzuca","year":"2014","journal-title":"Physiol. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.resp.2017.08.006","article-title":"Air blood barrier phenotype correlates with alveolo-capillary O2 equilibration in hypobaric hypoxia","volume":"246","author":"Beretta","year":"2017","journal-title":"Respir. Physiol. Neurobiol."}],"container-title":["Journal of Imaging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2313-433X\/5\/2\/22\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:27:51Z","timestamp":1760185671000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2313-433X\/5\/2\/22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,22]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2]]}},"alternative-id":["jimaging5020022"],"URL":"https:\/\/doi.org\/10.3390\/jimaging5020022","relation":{},"ISSN":["2313-433X"],"issn-type":[{"value":"2313-433X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,22]]}}}