{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:27:05Z","timestamp":1772252825129,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T00:00:00Z","timestamp":1562198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R15GM128166-01"],"award-info":[{"award-number":["1R15GM128166-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1727033"],"award-info":[{"award-number":["1727033"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Imaging"],"abstract":"<jats:p>Total internal reflection fluorescence microscopy with polarized excitation (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) primed with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE) coming into contact with a supported lipid bilayer containing mobile, monovalent DNP, modeling an immunological synapse. The spatial relationship of the IgE-bound high affinity IgE receptor (Fc\u03b5RI) to the ratio image of P-polarized excitation and S-polarized excitation was analyzed. These studies help correlate the dynamics of cell surface molecules with the mechanical properties of the plasma membrane during synapse formation.<\/jats:p>","DOI":"10.3390\/jimaging5070063","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T11:13:18Z","timestamp":1562238798000},"page":"63","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Imaging Membrane Curvature inside a Fc\u03b5RI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation"],"prefix":"10.3390","volume":"5","author":[{"given":"Rosa","family":"Machado","sequence":"first","affiliation":[{"name":"UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Bendesky","sequence":"additional","affiliation":[{"name":"UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madison","family":"Brown","sequence":"additional","affiliation":[{"name":"UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"},{"name":"Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5784-5253","authenticated-orcid":false,"given":"Kathrin","family":"Spendier","sequence":"additional","affiliation":[{"name":"UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"},{"name":"Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy M.","family":"Hagen","sequence":"additional","affiliation":[{"name":"UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/25764","article-title":"Three-dimensional segregation of supramolecular activation clusters in T cells","volume":"395","author":"Monks","year":"1998","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"20296","DOI":"10.1073\/pnas.0710258105","article-title":"Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells","volume":"104","author":"Kaizuka","year":"2007","journal-title":"Proc. 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