{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:48:15Z","timestamp":1760240895945,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T00:00:00Z","timestamp":1571875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000849","name":"National Centre for the Replacement, Refinement and Reduction of Animals in Research","doi-asserted-by":"publisher","award":["NC\/L001861\/1"],"award-info":[{"award-number":["NC\/L001861\/1"]}],"id":[{"id":"10.13039\/501100000849","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Imaging"],"abstract":"<jats:p>The spatial resolution and light detected in fluorescence imaging for small animals are limited by light scattering, absorption and autofluorescence. To address this, novel near-infrared fluorescent contrast agents and imaging configurations have been investigated. In this paper, the influence of the light wavelength and imaging configurations (full-field illumination system and scanning system) on fluorescence imaging are compared quantitatively. The surface radiance for both systems is calculated by modifying the simulation tool Near-Infrared Fluorescence and Spectral Tomography. Fluorescent targets are embedded within a scattering medium at different positions. The surface radiance and spatial resolution are obtained for emission wavelengths between 620 nm and 1000 nm. It was found that the spatial resolution of the scanning system is independent of the tissue optical properties, whereas for full-field illumination, the spatial resolution degrades at longer wavelength. The full width at half maximum obtained by the scanning system is 25% lower than that obtained by the full-field illumination system when the targets are located in the middle of the phantom. The results indicate that although imaging at near-infrared wavelength can achieve a higher surface radiance, it may produce worse spatial resolution.<\/jats:p>","DOI":"10.3390\/jimaging5110083","type":"journal-article","created":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T04:41:27Z","timestamp":1571978487000},"page":"83","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5811-8034","authenticated-orcid":false,"given":"Qimei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Engineering, Nottingham Trent University, Nottingham NG118PR, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1507-921X","authenticated-orcid":false,"given":"Anna M.","family":"Grabowska","sequence":"additional","affiliation":[{"name":"School of Medicine, Division of Cancer and Stem Cells, University of Nottingham, Nottingham NG72RD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip A.","family":"Clarke","sequence":"additional","affiliation":[{"name":"School of Medicine, Division of Cancer and Stem Cells, University of Nottingham, Nottingham NG72RD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4069-3801","authenticated-orcid":false,"given":"Stephen P.","family":"Morgan","sequence":"additional","affiliation":[{"name":"Optics and Photonics Group, Faculty of Engineering, University of Nottingham, Nottingham NG72RD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.ejrad.2009.01.049","article-title":"A review of molecular imaging studies reaching the clinical stage","volume":"70","author":"Wong","year":"2009","journal-title":"Eur. J. Radiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"104020","DOI":"10.1088\/0957-0233\/20\/10\/104020","article-title":"Review of biomedical optical imaging\u2014A powerful, noninvasive, non-ionizing technology for improving in vivo diagnosis","volume":"20","author":"Balas","year":"2009","journal-title":"Meas. Sci. Technol."},{"key":"ref_3","first-page":"635","article-title":"Biomedical Applications of Fluorescence Imaging In Vivo","volume":"54","author":"Hassan","year":"2004","journal-title":"Comp. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jphotobiol.2009.11.007","article-title":"Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applications","volume":"98","author":"Leblond","year":"2010","journal-title":"J. Photoch. Photobiol. B."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/nmeth.1483","article-title":"Going deeper than microscopy: The optical imaging frontier in biology","volume":"7","author":"Ntziachristos","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.cbpa.2009.10.022","article-title":"NIR dyes for bioimaging applications","volume":"14","author":"Escobedo","year":"2010","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1021\/cm2028367","article-title":"Review of Long-Wavelength Optical and NIR Imaging Materials: Contrast Agents, Fluorophores, and Multifunctional Nano Carriers","volume":"24","author":"Pansare","year":"2012","journal-title":"Chem. Mater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.1021\/acs.jmedchem.5b00253","article-title":"Tailored Near-Infrared Contrast Agents for Image Guided Surgery","volume":"58","author":"Njiojob","year":"2015","journal-title":"J. Med. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5560","DOI":"10.1039\/C6TB01234E","article-title":"Bright and stable near-infrared Pluronic-silica nanoparticles as contrast agents for in vivo optical imaging","volume":"4","author":"Yan","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.cbpa.2009.09.029","article-title":"Near-infrared fluorescence: Application to in vivo molecular imaging","volume":"14","author":"Hilderbrand","year":"2010","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"010506","DOI":"10.1117\/1.3299321","article-title":"Structured illumination enhances resolution and contrast in thick tissue fluorescence imaging","volume":"15","author":"Mazhar","year":"2010","journal-title":"J. Biomed. Opt."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"024012","DOI":"10.1117\/1.3088140","article-title":"Quantitation and mapping of tissue optical properties using modulated imaging","volume":"14","author":"Cuccia","year":"2009","journal-title":"J. Biomed. Opt."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1002\/lpor.200810031","article-title":"Sub-millimeter resolution 3D optical imaging of living tissue using laminar optical tomography","volume":"3","author":"Hillman","year":"2009","journal-title":"Laser Photonics Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"106003","DOI":"10.1117\/1.JBO.19.10.106003","article-title":"Laser line scanning for fluorescence reflectance imaging: A phantom study and in vivo validation of the enhancement of contrast and resolution","volume":"19","author":"Fantoni","year":"2014","journal-title":"J. Biomed. Opt."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"106003","DOI":"10.1117\/1.JBO.20.10.106003","article-title":"Laser line illumination scheme allowing the reduction of background signal and the correction of absorption heterogeneities effects for fluorescence reflectance imaging","volume":"20","author":"Fantoni","year":"2015","journal-title":"J. Biomed. Opt."},{"key":"ref_16","first-page":"711","article-title":"Near infrared optical tomography using NIRFAST: Algorithm for numerical model and image reconstruction","volume":"6","author":"Dehghani","year":"2008","journal-title":"Commun. Numer. Methods. Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1109\/TBME.2015.2405415","article-title":"Numerical Investigation of the Mechanisms of Ultrasound-Modulated Bioluminescence Tomography","volume":"62","author":"Zhang","year":"2015","journal-title":"IEEE. Trans. Biomed. Eng."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.ccr.2013.10.011","article-title":"Near-infrared emitting quantum dots: Recent progress on their synthesis and characterization","volume":"263\u2013264","author":"Pichaandi","year":"2014","journal-title":"Coord. Chem. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"024043-11","DOI":"10.1117\/1.3120493","article-title":"Ultrasound-modulated fluorescence based on a fluorophore-quencher-labeled microbubble system","volume":"14","author":"Yuan","year":"2009","journal-title":"J. Biomed. Opt."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1039\/c0an00063a","article-title":"Measurements for molar extinction coefficients of aqueous quantum dots","volume":"135","author":"Dong","year":"2010","journal-title":"Analyst"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"20171","DOI":"10.1021\/jp406774p","article-title":"Optical Properties of PbS\/CdS Core\/Shell Quantum Dots","volume":"117","author":"Justo","year":"2013","journal-title":"J. Phys. Chem. C"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"R37","DOI":"10.1088\/0031-9155\/58\/11\/R37","article-title":"Optical properties of biological tissues: A review","volume":"58","author":"Jacques","year":"2013","journal-title":"Phys. Med. Biol."},{"key":"ref_23","unstructured":"(1998, March 04). Tabulated Molar Extinction Coefficient for Hemoglobin in Water. Available online: http:\/\/omlc.org\/spectra\/hemoglobin\/summary.html."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1364\/JOSA.52.000276","article-title":"Application of Apodization to Increase Two-Point Resolution by the Sparrow Criterion. I. Coherent Illumination","volume":"52","author":"Barakat","year":"1962","journal-title":"J. Opt. Soc. Am."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1364\/OL.20.000426","article-title":"Experimental images of heterogeneous turbid media by frequency-domain diffusing-photon tomography","volume":"20","author":"Boas","year":"1995","journal-title":"Opt. Lett."},{"key":"ref_26","unstructured":"O\u2019Leary, M.A. (1995). Imaging with Diffuse Photon Density Waves. [Ph.D. Thesis, University of Pennsylvania]."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1364\/AO.35.001780","article-title":"Fluorescence spectroscopy of tissue: Recovery of intrinsic fluorescence from measured fluorescence","volume":"35","author":"Gardner","year":"1996","journal-title":"Appl. Opt."},{"key":"ref_28","unstructured":"Zhang, F. (2015). Theoretical Examinations of Optical Tomography through Scattering Medium. [Ph.D. Thesis, University of Nottingham]."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0066319","article-title":"Depth Sensitivity and Source-Detector Separations for Near Infrared Spectroscopy Based on the Colin27 Brain Template","volume":"8","author":"Strangman","year":"2013","journal-title":"PlOS ONE"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kawaguchi, H., Hayashi, T., Kato, T., and Okada, E. (2003, January 22). Evaluation of spatial resolution of near-infrared topography using spatial sensitivity profile. Proceedings of the European Conference on Biomedical Optics, Munich, Germany.","DOI":"10.1364\/ECBO.2003.5138_249"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"R3138","DOI":"10.1149\/2.0171601jss","article-title":"Applications of Highly Bright PbS Quantum Dots to Non-Invasive Near-Infrared Fluorescence Imaging in the Second OpticalWindow","volume":"5","author":"Jin","year":"2016","journal-title":"ECS J. Solid State Sci. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Bai, M. (2016). In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes. Vivo Fluorescence Imaging: Methods and Protocols, Springer.","DOI":"10.1007\/978-1-4939-3721-9"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4077","DOI":"10.1364\/BOE.8.004077","article-title":"Ballistic and snake photon imaging for locating optical endomicroscopy fibres","volume":"8","author":"Tanner","year":"2017","journal-title":"Biomed. Opt. Express"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5495","DOI":"10.1364\/AO.54.005495","article-title":"Optimization of galvanometer scanning for optical coherence tomography","volume":"54","author":"Duma","year":"2015","journal-title":"Appl. Opt."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1117\/1.1413210","article-title":"In vivo imaging of light-emitting probes","volume":"6","author":"Rice","year":"2001","journal-title":"J. Biomed. Opt."},{"key":"ref_36","unstructured":"Industries, B. (1980). Photomultiplier Handbook, Burle Industries."}],"container-title":["Journal of Imaging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2313-433X\/5\/11\/83\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:29:07Z","timestamp":1760189347000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2313-433X\/5\/11\/83"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,24]]},"references-count":36,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2019,11]]}},"alternative-id":["jimaging5110083"],"URL":"https:\/\/doi.org\/10.3390\/jimaging5110083","relation":{},"ISSN":["2313-433X"],"issn-type":[{"type":"electronic","value":"2313-433X"}],"subject":[],"published":{"date-parts":[[2019,10,24]]}}}