{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T15:29:34Z","timestamp":1780759774772,"version":"3.54.1"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Imaging"],"abstract":"<jats:p>The 64Cu-labeled chelator was analyzed in vivo by positron emission tomography (PET) imaging to evaluate its biodistribution in a murine model at different acquisition times. For this purpose, nine 6-week-old female Balb\/C nude strain mice underwent micro-PET imaging at three different time points after 64Cu-labeled chelator injection. Specifically, the mice were divided into group 1 (acquisition 1 h after [64Cu] chelator administration, n = 3 mice), group 2 (acquisition 4 h after [64Cu]chelator administration, n = 3 mice), and group 3 (acquisition 24 h after [64Cu] chelator administration, n = 3 mice). Successively, all PET studies were segmented by means of registration with a standard template space (3D whole-body Digimouse atlas), and 108 radiomics features were extracted from seven organs (namely, heart, bladder, stomach, liver, spleen, kidney, and lung) to investigate possible changes over time in [64Cu]chelator biodistribution. The one-way analysis of variance and post hoc Tukey Honestly Significant Difference test revealed that, while heart, stomach, spleen, kidney, and lung districts showed a very low percentage of radiomics features with significant variations (p-value &lt; 0.05) among the three groups of mice, a large number of features (greater than 60% and 50%, respectively) that varied significantly between groups were observed in bladder and liver, indicating a different in vivo uptake of the 64Cu-labeled chelator over time. The proposed methodology may improve the method of calculating the [64Cu]chelator biodistribution and open the way towards a decision support system in the field of new radiopharmaceuticals used in preclinical imaging trials.<\/jats:p>","DOI":"10.3390\/jimaging8040092","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T21:28:39Z","timestamp":1648675719000},"page":"92","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["A New Preclinical Decision Support System Based on PET Radiomics: A Preliminary Study on the Evaluation of an Innovative 64Cu-Labeled Chelator in Mouse Models"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3629-355X","authenticated-orcid":false,"given":"Viviana","family":"Benfante","sequence":"first","affiliation":[{"name":"Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), 90015 Cefal\u00f9, Italy"},{"name":"Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, Molecular and Clinical Medicine, University of Palermo, 90127 Palermo, Italy"},{"name":"Ri.MED Foundation, Via Bandiera 11, 90133 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7189-1731","authenticated-orcid":false,"given":"Alessandro","family":"Stefano","sequence":"additional","affiliation":[{"name":"Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), 90015 Cefal\u00f9, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9290-6103","authenticated-orcid":false,"given":"Albert","family":"Comelli","sequence":"additional","affiliation":[{"name":"Ri.MED Foundation, Via Bandiera 11, 90133 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Giaccone","sequence":"additional","affiliation":[{"name":"Ri.MED Foundation, Via Bandiera 11, 90133 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0554-6649","authenticated-orcid":false,"given":"Francesco Paolo","family":"Cammarata","sequence":"additional","affiliation":[{"name":"Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), 90015 Cefal\u00f9, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2540-5568","authenticated-orcid":false,"given":"Selene","family":"Richiusa","sequence":"additional","affiliation":[{"name":"Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), 90015 Cefal\u00f9, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Scopelliti","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Department, Cannizzaro Hospital, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Pometti","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Department, Cannizzaro Hospital, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milene","family":"Ficarra","sequence":"additional","affiliation":[{"name":"Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), 90015 Cefal\u00f9, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastiano","family":"Cosentino","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Department, Cannizzaro Hospital, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6027-0024","authenticated-orcid":false,"given":"Marcello","family":"Lunardon","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Padova, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2499-5490","authenticated-orcid":false,"given":"Francesca","family":"Mastrotto","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharamacological Sciences, University of Padova, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Andrighetto","sequence":"additional","affiliation":[{"name":"Legnaro National Laboratories, National Institute of Nuclear Physics, 35020 Legnaro, 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