{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T23:59:28Z","timestamp":1777075168838,"version":"3.51.4"},"reference-count":10,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Imaging"],"abstract":"<jats:p>Introduction and objective: Nowadays, investigations of heart physiology and pathophysiology rely more and more upon image analysis, whether for the detection and characterization of events in single cells or for the mapping of events and their characteristics across an entire tissue. These investigations require extensive skills in image analysis and\/or expensive software, and their reproducibility may be a concern. Our objective was to build a robust, reliable and open-source software tool to quantify excitation\u2013contraction related experimental data at multiple scales, from single isolated cells to the whole heart. Methods and results: A free and open-source ImageJ plugin, Spiky, was developed to detect and analyze peaks in experimental data streams. It allows rapid and easy analysis of action potentials, intracellular calcium transient and contraction data from cardiac research experiments. As shown in the provided examples, both classical bi-dimensional data (XT signals) and video data obtained from confocal microscopy and optical mapping experiments (XYT signals) can be analyzed. Spiky was written in ImageJ Macro Language and JAVA, and works under Windows, Mac and Linux operating systems. Conclusion: Spiky provides a complete working interface to process and analyze cardiac physiology research data.<\/jats:p>","DOI":"10.3390\/jimaging8040095","type":"journal-article","created":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T21:22:39Z","timestamp":1648848159000},"page":"95","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Spiky: An ImageJ Plugin for Data Analysis of Functional Cardiac and Cardiomyocyte Studies"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8625-1617","authenticated-orcid":false,"given":"C\u00f4me","family":"Pasqualin","sequence":"first","affiliation":[{"name":"Groupe Physiologie des Cellules Cardiaques et Vasculaires, Universit\u00e9 de Tours, EA4245 Transplantation, Immunologie, Inflammation, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Gannier","sequence":"additional","affiliation":[{"name":"Groupe Physiologie des Cellules Cardiaques et Vasculaires, Universit\u00e9 de Tours, EA4245 Transplantation, Immunologie, Inflammation, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ang\u00e8le","family":"Yu","sequence":"additional","affiliation":[{"name":"Groupe Physiologie des Cellules Cardiaques et Vasculaires, Universit\u00e9 de Tours, EA4245 Transplantation, Immunologie, Inflammation, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Benoist","sequence":"additional","affiliation":[{"name":"IHU Liryc, Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Universit\u00e9, 33600 Pessac, France"},{"name":"Universite de Bordeaux, Inserm, Centre de Recherche Cardio-Thoracique, U1045, 33000 Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Findlay","sequence":"additional","affiliation":[{"name":"Groupe Physiologie des Cellules Cardiaques et Vasculaires, Universit\u00e9 de Tours, EA4245 Transplantation, Immunologie, Inflammation, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2972-688X","authenticated-orcid":false,"given":"Romain","family":"Bordy","sequence":"additional","affiliation":[{"name":"Groupe Physiologie des Cellules Cardiaques et Vasculaires, Universit\u00e9 de Tours, EA4245 Transplantation, Immunologie, Inflammation, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0905-0680","authenticated-orcid":false,"given":"Pierre","family":"Bredeloux","sequence":"additional","affiliation":[{"name":"Groupe Physiologie des Cellules Cardiaques et Vasculaires, Universit\u00e9 de Tours, EA4245 Transplantation, Immunologie, Inflammation, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e9ronique","family":"Maupoil","sequence":"additional","affiliation":[{"name":"Groupe Physiologie des Cellules Cardiaques et Vasculaires, Universit\u00e9 de Tours, EA4245 Transplantation, Immunologie, Inflammation, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"H1388","DOI":"10.1152\/ajpheart.00665.2015","article-title":"A Technical Review of Optical Mapping of Intracellular Calcium within Myocardial Tissue","volume":"310","author":"Jaimes","year":"2016","journal-title":"Am. 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