{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T13:25:58Z","timestamp":1768569958064,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T00:00:00Z","timestamp":1386028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic\/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed.<\/jats:p>","DOI":"10.3390\/mi4040402","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T11:15:30Z","timestamp":1386069330000},"page":"402-413","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Monodisperse Water-in-Oil-in-Water (W\/O\/W) Double Emulsion Droplets as Uniform Compartments for  High-Throughput Analysis via Flow Cytometry"],"prefix":"10.3390","volume":"4","author":[{"given":"Jing","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, 80 Tennis Court Road,  Cambridge CB2 1GA, UK"},{"name":"Department of Chemical Engineering and Biotechnology, University of Cambridge,  Pembroke Street, Cambridge CB2 3RA, UK"}]},{"given":"Wolfgang-Andreas","family":"Bauer","sequence":"additional","affiliation":[{"name":"Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK"}]},{"given":"Martin","family":"Fischlechner","sequence":"additional","affiliation":[{"name":"Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UK"}]},{"given":"Florian","family":"Hollfelder","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, 80 Tennis Court Road,  Cambridge CB2 1GA, UK"}]},{"given":"Clemens","family":"Kaminski","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology, University of Cambridge,  Pembroke Street, Cambridge CB2 3RA, UK"}]},{"given":"Wilhelm","family":"Huck","sequence":"additional","affiliation":[{"name":"Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK"},{"name":"Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen 6525 AJ, The Netherlands"}]}],"member":"1968","published-online":{"date-parts":[[2013,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1021\/j150252a009","article-title":"Study of emulsions","volume":"29","author":"Seifritz","year":"1925","journal-title":"J. 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Pharmaceut."},{"key":"ref_5","first-page":"239","article-title":"Preparation of a new soft capsule for cosmetics","volume":"51","author":"Miyazawa","year":"2005","journal-title":"J. Cosmet. Sci."},{"key":"ref_6","first-page":"739","article-title":"Preparation of water-in-olive oil-in-water multiple-phase emulsions in an eatable form","volume":"42","author":"Matsumoto","year":"1978","journal-title":"Agric. Biol. Chem."},{"key":"ref_7","unstructured":"Dittrich, W., and G\u00f6hde, W. (1968). Flow-Through Chamber for Photometers to Measure and Count Particles in a Dispersion Medium. (DE 1815352 (A1))."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2232","DOI":"10.1039\/b814954b","article-title":"Size and fluorescence measurements of individual droplets by flow cytometry","volume":"5","author":"Fattaccioli","year":"2009","journal-title":"Soft Matter"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1093\/emboj\/cdg014","article-title":"Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization","volume":"22","author":"Griffiths","year":"2003","journal-title":"EMBO J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.ab.2003.10.005","article-title":"In vitro compartmentalization by double emulsions: Sorting and gene enrichment by fluorescence activated cell sorting","volume":"325","author":"Bernath","year":"2004","journal-title":"Anal. Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1016\/j.chembiol.2005.09.012","article-title":"High-throughput screening of enzyme libraries: Thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments","volume":"12","author":"Aharoni","year":"2005","journal-title":"Chem. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1016\/j.chembiol.2005.09.016","article-title":"High-throughput screening of enzyme libraries: In vitro evolution of a \u03b2-galactosidase by fluorescence-activated sorting of double emulsions","volume":"12","author":"Mastrobattista","year":"2005","journal-title":"Chem. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.sbi.2005.07.006","article-title":"New genotype-phenotype linkages for directed evolution of functional proteins","volume":"15","author":"Leemhuis","year":"2005","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1039\/b907578j","article-title":"The potential of microfluidic water-in-oil droplets in experimental biology","volume":"5","author":"Schaerli","year":"2009","journal-title":"Mol. Biosyst."},{"key":"ref_15","unstructured":"Schaerli, Y., Kintses, B., Hollfelder, F., Lutz, S., and Bornscheuer, U.T. (2012). Protein Engineering Handbook, Wiley VCH."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4004","DOI":"10.1073\/pnas.0910781107","article-title":"Ultrahigh-throughput screening in drop-based microfluidics for directed evolution","volume":"107","author":"Agresti","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.chembiol.2012.06.009","article-title":"Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution","volume":"19","author":"Kintses","year":"2012","journal-title":"Chem. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1039\/b902504a","article-title":"Fluorescence-activated droplet sorting (FADS): Efficient microfluidic cell sorting based on enzymatic activity","volume":"9","author":"Baret","year":"2009","journal-title":"Lab Chip"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1002\/adma.19960081110","article-title":"Rapid prototyping of complex structures with feature sizes larger than 20 \u03bcm","volume":"8","author":"Qin","year":"1996","journal-title":"Adv. Mater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4974","DOI":"10.1021\/ac980656z","article-title":"Rapid prototyping of microfluidic systems in poly(dimethylsiloxane)","volume":"70","author":"Duffy","year":"1998","journal-title":"Anal. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1039\/c004046k","article-title":"Hydrophilic PDMS microchannels for high-throughput formation of oil-in-water microdroplets and water-in-oil-in-water double emulsions","volume":"10","author":"Bauer","year":"2010","journal-title":"Lab Chip"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4214","DOI":"10.1039\/c1sm05087g","article-title":"Microfluidic production of monodisperse functional o\/w droplets and study of their reversible pH dependent aggregation behavior","volume":"7","author":"Bauer","year":"2011","journal-title":"Soft Matter"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2624","DOI":"10.1002\/adfm.201200324","article-title":"Formation of spherical and non-spherical eutectic gallium-indium liquid-metal microdroplets in microfluidic channels at room temperature","volume":"22","author":"Hutter","year":"2012","journal-title":"Adv. Funct. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1039\/b806706f","article-title":"Biocompatible surfactants for water-in-fluorocarbon emulsions","volume":"8","author":"Holtze","year":"2008","journal-title":"Lab Chip"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1063\/1.1537519","article-title":"Formation of dispersions using \u201cflow focusing\u201d in microchannels","volume":"82","author":"Anna","year":"2003","journal-title":"Appl. Phys. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1039\/b501972a","article-title":"Controlled formulation of monodisperse double emulsions in a multiple-phase microfluidic system","volume":"1","author":"Nisisako","year":"2005","journal-title":"Soft Matter"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1002\/smll.200900569","article-title":"High-order multiple emulsions formed in poly(dimethylsiloxane) microfluidics","volume":"5","author":"Abate","year":"2009","journal-title":"Small"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1039\/c004050a","article-title":"Functional patterning of PDMS microfluidic devices using integrated chemo-masks","volume":"10","author":"Romanowsky","year":"2010","journal-title":"Lab Chip"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1023\/A:1022586127784","article-title":"Use of FITC as a fluorescent probe for intracellular pH measurement","volume":"7","author":"Lanz","year":"1997","journal-title":"J. Fluoresc."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lakowicz, J.R. (2006). Principles of Fluorescence Spectroscopy, Springer. [3rd ed.].","DOI":"10.1007\/978-0-387-46312-4"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5846","DOI":"10.1002\/anie.200906653","article-title":"Microdroplets in microfluidics: An evolving platform for discoveries in chemistry and biology","volume":"49","author":"Theberge","year":"2010","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.cbpa.2010.08.013","article-title":"Microfluidic droplets: New integrated workflows for biological experiments","volume":"14","author":"Kintses","year":"2010","journal-title":"Curr. Opin. Chem. 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