{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:05:30Z","timestamp":1763726730378,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T00:00:00Z","timestamp":1386028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 \u03bcm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min\u22121, moderate = 50 nL min\u22121, and high = 100 nL min\u22121 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.<\/jats:p>","DOI":"10.3390\/mi4040414","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T11:15:30Z","timestamp":1386069330000},"page":"414-430","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Hydrodynamic Cell Trapping for High Throughput  Single-Cell Applications"],"prefix":"10.3390","volume":"4","author":[{"given":"Amin","family":"Banaeiyan","sequence":"first","affiliation":[{"name":"Department of Physics, University of Gothenburg, Gothenburg, SE-412 96, Sweden"}]},{"given":"Doryaneh","family":"Ahmadpour","sequence":"additional","affiliation":[{"name":"Department of Physics, University of Gothenburg, Gothenburg, SE-412 96, Sweden"}]},{"given":"Caroline","family":"Adiels","sequence":"additional","affiliation":[{"name":"Department of Physics, University of Gothenburg, Gothenburg, SE-412 96, Sweden"}]},{"given":"Mattias","family":"Goks\u00f6r","sequence":"additional","affiliation":[{"name":"Department of Physics, University of Gothenburg, Gothenburg, SE-412 96, Sweden"}]}],"member":"1968","published-online":{"date-parts":[[2013,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"330A","DOI":"10.1021\/ac002800y","article-title":"Lab-on-a-chip: A revolution in biological and medical sciences","volume":"72","author":"Figeys","year":"2000","journal-title":"Anal. 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