{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:41:45Z","timestamp":1760218905970,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2014,2,19]],"date-time":"2014-02-19T00:00:00Z","timestamp":1392768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>We present a single cell study of a highly effective Hog1 inhibitor. For this application, we used sequential treatment of a Saccharomyces cerevisiae cell array, with the Hog1 inhibitor and osmotic stress. For this purpose, a four-inlet microfluidic chamber with controlled introduction of two different cell strains within the same experimental setting and a subsequent rapid switching between treatments was designed. Multiple cell strains within the same experiment is a unique feature which is necessary for determining the expected absent cellular response. The nuclear translocation of the cytosolic MAPK, Hog1, was monitored by fluorescence imaging of Hog1-GFP on a single-cell level.  An optical tweezers setup was used for controlled cell capture and array formation.  Nuclear Hog1-GFP localization was impaired for treated cells, providing evidence of a congenial microfluidic setup, where the control cells within the experiments validated its appropriateness. The chamber enables multiple treatments with incubation times in the order of seconds and the possibility to remove either of the treatments during measurement. This flexibility and the possibility to use internal control cells ensures it a valuable scientific tool for unraveling the HOG pathway, similar signal transduction pathways  and other biological mechanisms where temporal resolution and real time imaging is  a prerequisite.<\/jats:p>","DOI":"10.3390\/mi5010081","type":"journal-article","created":{"date-parts":[[2014,2,19]],"date-time":"2014-02-19T11:10:21Z","timestamp":1392808221000},"page":"81-96","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Single-Cell Study of a Highly Effective Hog1 Inhibitor for  in Situ Yeast Cell Manipulation"],"prefix":"10.3390","volume":"5","author":[{"given":"Charlotte","family":"Blomqvist","sequence":"first","affiliation":[{"name":"Department of Physics, University of Gothenburg, G\u00f6teborg S-412 96, Sweden"},{"name":"Department of Applied Physics, Chalmers University of Technology, G\u00f6teborg S-412 96, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Din\u00e9r","sequence":"additional","affiliation":[{"name":"Department of Chemistry, KTH-Royal Institute of Technology, Teknikringen 30, SE-10044 Stockholm, Sweden"},{"name":"Department of Chemistry and Molecular Biology, University of Gothenburg,  G\u00f6teborg S-413 90, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morten","family":"Gr\u00f8tli","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Molecular Biology, University of Gothenburg,  G\u00f6teborg S-413 90, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mattias","family":"Goks\u00f6r","sequence":"additional","affiliation":[{"name":"Department of Physics, University of Gothenburg, G\u00f6teborg S-412 96, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Adiels","sequence":"additional","affiliation":[{"name":"Department of Physics, University of Gothenburg, G\u00f6teborg S-412 96, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1038\/166956a0","article-title":"Direct demonstration of the mutagenic action of euflavine on baker\u2019s yeast","volume":"166","author":"Ephrussi","year":"1950","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1039\/c0lc00150c","article-title":"Overview of single-cell analyses: Microdevices and applications","volume":"10","author":"Lindstrom","year":"2010","journal-title":"Lab Chip"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1039\/b704632b","article-title":"Single cells or large populations?","volume":"7","author":"Svahn","year":"2007","journal-title":"Lab Chip"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7918","DOI":"10.1021\/ac069490p","article-title":"Dynamic single-cell analysis for quantitative biology","volume":"78","author":"Lee","year":"2006","journal-title":"Anal. 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