{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T20:31:59Z","timestamp":1778358719982,"version":"3.51.4"},"reference-count":28,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T00:00:00Z","timestamp":1398124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Circulating tumor cells (CTCs) from blood of cancer patients are valuable prognostic markers and enable monitoring responses to therapy. The extremely low number of CTCs makes their isolation and characterization a major technological challenge. For label-free cell identification a novel combination of Raman spectroscopy with a microhole array platform is described that is expected to support high-throughput and multiplex analyses. Raman spectra were registered from regularly arranged cells on the chip with low background noise from the silicon nitride chip membrane. A classification model was trained to distinguish leukocytes from myeloblasts (OCI-AML3) and breast cancer cells (MCF-7 and BT-20). The model was validated by Raman spectra of a mixed cell population. The high spectral quality, low destructivity and high classification accuracy suggests that this approach is promising for Raman activated cell sorting.<\/jats:p>","DOI":"10.3390\/mi5020204","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T12:18:37Z","timestamp":1398169117000},"page":"204-215","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Raman-Spectroscopy Based Cell Identification on a Microhole Array Chip"],"prefix":"10.3390","volume":"5","author":[{"given":"Ute","family":"Neugebauer","sequence":"first","affiliation":[{"name":"Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, D-07745 Jena, Germany"},{"name":"Center for Sepsis Control and Care (CSCC), Jena University Hospital, Erlanger Allee 101,  D-07747 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Kurz","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Biomedical Engineering (IBMT), Ensheimer Stra\u00dfe 48, D-66386 St. Ingbert, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Bocklitz","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry and Abbe Center of Photonic, Friedrich-Schiller-University Jena, Helmholtzweg 4, D-07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, Haematology and Medical Oncology, Jena University Hospital, Erlanger Allee 101, D-07747 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Velten","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Biomedical Engineering (IBMT), Ensheimer Stra\u00dfe 48, D-66386 St. Ingbert, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Clement","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, Haematology and Medical Oncology, Jena University Hospital, Erlanger Allee 101, D-07747 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Krafft","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, D-07745 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Popp","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, D-07745 Jena, Germany"},{"name":"Center for Sepsis Control and Care (CSCC), Jena University Hospital, Erlanger Allee 101,  D-07747 Jena, Germany"},{"name":"Institute of Physical Chemistry and Abbe Center of Photonic, Friedrich-Schiller-University Jena, Helmholtzweg 4, D-07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1083\/jcb.201010021","article-title":"Circulating tumor cells: Approaches to isolation and characterization","volume":"192","author":"Yu","year":"2011","journal-title":"J. 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