{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T07:33:26Z","timestamp":1767339206449,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T00:00:00Z","timestamp":1435536000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>When Raman microscopy is adopted to detect the chemical and biological processes in the silicon microfluidic channel, the laser-induced heating effect will cause a temperature rise in the sample liquid. This undesired temperature rise will mislead the Raman measurement during the temperature-influencing processes. In this paper, computational fluid dynamics simulations were conducted to evaluate the maximum local temperature-rise (MLT). Through the orthogonal analysis, the sensitivity of potential influencing parameters to the MLT was determined. In addition, it was found from transient simulations that it is reasonable to assume the actual measurement to be  steady-state. Simulation results were qualitatively validated by experimental data from the Raman measurement of diffusion, a temperature-dependent process. A correlation was proposed for the first time to estimate the MLT. Simple in form and convenient for calculation, this correlation can be efficiently applied to Raman measurement in a silicon microfluidic channel.<\/jats:p>","DOI":"10.3390\/mi6070813","type":"journal-article","created":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T10:05:22Z","timestamp":1435572322000},"page":"813-830","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of Laser-Induced Heating on Raman Measurement within a Silicon Microfluidic Channel"],"prefix":"10.3390","volume":"6","author":[{"given":"Ying","family":"Lin","sequence":"first","affiliation":[{"name":"School of Mechanical Engineering, Shanghai Institute of Technology, Shanghai 201418, China"}]},{"given":"Xinhai","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pressurized Systems and Safety, Ministry of Education, School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, China"}]},{"given":"Zhenyu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Software and Microelectronics at Wuxi, Peking University, Wuxi 214125, China"}]},{"given":"Shan-Tung","family":"Tu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pressurized Systems and Safety, Ministry of Education, School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, China"}]},{"given":"Zhengdong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pressurized Systems and Safety, Ministry of Education, School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, China"}]}],"member":"1968","published-online":{"date-parts":[[2015,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5340","DOI":"10.1021\/ac990504j","article-title":"Quantitative analysis of molecular interaction in a microfluidic channel: The T-sensor","volume":"71","author":"Kamholz","year":"1999","journal-title":"Anal. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/nature05062","article-title":"Control and detection of chemical reactions in microfluidic systems","volume":"442","author":"DeMello","year":"2006","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5646","DOI":"10.1021\/ac0346510","article-title":"Microfluidic devices for the high-throughput chemical analysis of cells","volume":"75","author":"McClain","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2902","DOI":"10.1039\/b907753g","article-title":"Multi-step microfluidic droplet processing: Kinetic analysis of an in vitro translated enzyme","volume":"9","author":"Mazutis","year":"2009","journal-title":"Lab Chip"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4443","DOI":"10.1002\/elps.200800121","article-title":"Microfluidics: Applications for analytical purposes in chemistry and biochemistry","volume":"29","author":"Ohno","year":"2008","journal-title":"Electrophoresis"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s10404-013-1223-8","article-title":"Characterization of a new system for space-resolved simultaneous in situ measurements of hydrocarbons and dissolved oxygen in microchannels","volume":"16","author":"Rinke","year":"2014","journal-title":"Microfluid. Nanofluid."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1021\/ac902515c","article-title":"Microbioreactors for raman microscopy of stromal cell differentiation","volume":"82","author":"Pully","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1021\/ac802410g","article-title":"Design and evaluation of a passive alcove-based microfluidic mixer","volume":"81","author":"Egawa","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1021\/ac7020147","article-title":"Chemical reaction imaging within microfluidic devices using confocal raman spectroscopy: The case of water and deuterium oxide as a model system","volume":"80","author":"Sarrazin","year":"2008","journal-title":"Anal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3239","DOI":"10.1002\/elps.200305532","article-title":"Monitoring of chemical reactions within microreactors using an inverted raman microscopic spectrometer","volume":"24","author":"Fletcher","year":"2003","journal-title":"Electrophoresis"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1039\/C4AY00046C","article-title":"Brushing, a simple way to fabricate sers active paper substrates","volume":"6","author":"Zhang","year":"2014","journal-title":"Anal. Methods"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"9035","DOI":"10.1021\/nn502294w","article-title":"Photothermal heating enabled by plasmonic nanostructures for electrokinetic manipulation and sorting of particles","volume":"8","author":"Ndukaife","year":"2014","journal-title":"ACS Nano"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1021\/cr300120g","article-title":"Sers tags: Novel optical nanoprobes for bioanalysis","volume":"113","author":"Wang","year":"2013","journal-title":"Chem. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"13015","DOI":"10.1039\/c3ra40610e","article-title":"Surface-enhanced raman scattering microfluidic sensor","volume":"3","author":"Li","year":"2013","journal-title":"RSC Adv."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1366\/0003702001950382","article-title":"Modeling and measuring the effect of refraction on the depth resolution of confocal raman microscopy","volume":"54","author":"Everall","year":"2000","journal-title":"Appl. Spectrosc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1366\/0003702001948439","article-title":"Confocal raman microscopy: Why the depth resolution and spatial accuracy can be much worse than you think","volume":"54","author":"Everall","year":"2000","journal-title":"Appl. Spectrosc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"094106","DOI":"10.1063\/1.1873050","article-title":"In situ raman imaging of interdiffusion in a microchannel","volume":"86","author":"Salmon","year":"2005","journal-title":"Appl. Phys. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.aca.2010.03.061","article-title":"Measurement of temperature-dependent diffusion coefficients using a confocal raman microscope with microfluidic chips considering laser-induced heating effect","volume":"667","author":"Lin","year":"2010","journal-title":"Anal. Chim. Acta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1063\/1.327809","article-title":"Raman measurements of temperature during cw laser heating of silicon","volume":"51","author":"Lo","year":"1980","journal-title":"J. Appl. Phys."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4946","DOI":"10.1063\/1.1611282","article-title":"Influence of local heating on micro-raman spectroscopy of silicon","volume":"94","author":"Arguirov","year":"2003","journal-title":"J. Appl. Phys."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.mejo.2006.09.007","article-title":"Micro-raman spectroscopy measurement of stress in silicon","volume":"38","author":"Wu","year":"2007","journal-title":"Microelectron. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"123104","DOI":"10.1063\/1.2743882","article-title":"Effects of laser-induced heating on raman stress measurements of silicon and silicon-germanium structures","volume":"101","author":"Georgi","year":"2007","journal-title":"J. Appl. Phys."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Suthar, K.J., Patten, J., Dong, L., and Abdel-Aal, H. (2008, January 7\u201310). Estimation of Temperature Distribution in Silicon During Micro Laser Assisted Machining. Proceedings of the ASME 2008 International Manufacturing Science and Engineering Conference, Evanston, IL, USA.","DOI":"10.1115\/MSEC_ICMP2008-72195"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5216","DOI":"10.1364\/OE.14.005216","article-title":"Heating effects in tip-enhanced optical microscopy","volume":"14","author":"Downes","year":"2006","journal-title":"Opt. Express"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.mcp.2012.03.008","article-title":"Single DNA molecule denaturation using laser-induced heating","volume":"26","author":"Hung","year":"2012","journal-title":"Mol. Cell. Probes"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1039\/b817288a","article-title":"Petri dish pcr: Laser-heated reactions in nanoliter droplet arrays","volume":"9","author":"Kim","year":"2009","journal-title":"Lab Chip"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1016\/j.bpj.2010.07.019","article-title":"Laser-assisted single-molecule refolding (lasr)","volume":"99","author":"Zhao","year":"2010","journal-title":"Biophys. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"133901","DOI":"10.1063\/1.2790806","article-title":"Melting curve analysis in a snapshot","volume":"91","author":"Baaske","year":"2007","journal-title":"Appl. Phys. Lett."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1002\/jrs.1736","article-title":"Confocal raman microscopy: How to correct depth profiles considering diffraction and refraction effects","volume":"38","author":"Gallardo","year":"2007","journal-title":"J. Raman Spectrosc."},{"key":"ref_30","first-page":"16","article-title":"Depth profiling with confocal raman microscopy, part II","volume":"19","author":"Everall","year":"2004","journal-title":"Spectroscopy"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1366\/0003702011952190","article-title":"Confocal raman microspectroscopy through a planar interface","volume":"55","author":"Baldwin","year":"2001","journal-title":"Appl. Spectrosc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1002\/jrs.915","article-title":"In-depth analyses by confocal raman microspectrometry: Experimental features and modeling of the refraction effects","volume":"33","author":"Bruneel","year":"2002","journal-title":"J. Raman Spectrosc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2551","DOI":"10.1016\/j.ijheatmasstransfer.2004.01.008","article-title":"Effect of axial conduction on the heat transfer in micro-channels","volume":"47","author":"Tiselj","year":"2004","journal-title":"Int. J. Heat Mass Transf."},{"key":"ref_34","unstructured":"Engineering ToolBox. Available online:http:\/\/www.engineeringtoolbox.com."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3993","DOI":"10.1016\/j.ijheatmasstransfer.2004.04.016","article-title":"Mini- and micro-channels: Influence of axial conduction in the walls","volume":"47","author":"Maranzana","year":"2004","journal-title":"Int. J. Heat Mass Transf."}],"container-title":["Micromachines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-666X\/6\/7\/813\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:48:31Z","timestamp":1760215711000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-666X\/6\/7\/813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,29]]},"references-count":35,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2015,7]]}},"alternative-id":["mi6070813"],"URL":"https:\/\/doi.org\/10.3390\/mi6070813","relation":{},"ISSN":["2072-666X"],"issn-type":[{"type":"electronic","value":"2072-666X"}],"subject":[],"published":{"date-parts":[[2015,6,29]]}}}