{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T04:35:15Z","timestamp":1769834115312,"version":"3.49.0"},"reference-count":24,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2016,2,17]],"date-time":"2016-02-17T00:00:00Z","timestamp":1455667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Spinning-disc interferometry (SDI) is a high-speed laser scanning approach to surface metrology that uses common-path interferometry to measure protein spots on a BioCD disk. The measurement sensitivity depends on the scanning pitch and on the time-base. Based on high-resolution laser scanning images of printed antibody spots, we quantify the protein sensitivity as a function of the scan parameters. For smoothly printed antibody spots scanned with a transverse spatial resolution of 1 \u03bcm, the surface height precision for a single 100 \u03bcm diameter protein spot is approximately 1 pm. This detection sensitivity sets the fundamental limit of detection for label-free BioCD biosensors performing immunoassays.<\/jats:p>","DOI":"10.3390\/mi7020031","type":"journal-article","created":{"date-parts":[[2016,2,18]],"date-time":"2016-02-18T22:19:47Z","timestamp":1455833987000},"page":"31","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD"],"prefix":"10.3390","volume":"7","author":[{"given":"Kevin","family":"O\u2019Brien","sequence":"first","affiliation":[{"name":"Department of Physics, University of California Berkeley, Berkeley, CA 94720-7300, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Physics, Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Nolte","sequence":"additional","affiliation":[{"name":"Department of Physics, Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1364\/OL.29.000950","article-title":"Spinning-disk self-referencing interferometry of antigen-antibody recognition","volume":"29","author":"Varma","year":"2004","journal-title":"Opt. 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