{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:19:51Z","timestamp":1760242791415,"version":"build-2065373602"},"reference-count":20,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T00:00:00Z","timestamp":1466380800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 61403322"],"award-info":[{"award-number":["No. 61403322"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Natural Science Foundation of Guangdong Province","award":["2014A030310318"],"award-info":[{"award-number":["2014A030310318"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Robot-assisted cell manipulation is gaining attention for its ability in providing high throughput and high precision cell manipulation for the biological industry. This paper presents a visual servo microrobotic system for cell microinjection. We investigated the automatic cell autofocus method that reduced the complexity of the system. Then, we produced an adaptive visual processing algorithm to detect the location of the cell and micropipette toward the uneven illumination problem. Fourteen microinjection experiments were conducted with zebrafish embryos. A 100% success rate was achieved either in autofocus or embryo detection, which verified the robustness of the proposed automatic cell manipulation system.<\/jats:p>","DOI":"10.3390\/mi7060104","type":"journal-article","created":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T12:51:28Z","timestamp":1466427088000},"page":"104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Robot-Assisted Cell Manipulation System with an Adaptive Visual Servoing Method"],"prefix":"10.3390","volume":"7","author":[{"given":"Yu","family":"Xie","sequence":"first","affiliation":[{"name":"School of Aerospace Engineering, Xiamen University, Xiamen 361005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8685-7360","authenticated-orcid":false,"given":"Feng","family":"Zeng","sequence":"additional","affiliation":[{"name":"School of Aerospace Engineering, Xiamen University, Xiamen 361005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenming","family":"Xi","sequence":"additional","affiliation":[{"name":"School of Aerospace Engineering, Xiamen University, Xiamen 361005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunlei","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Aerospace Engineering, Xiamen University, Xiamen 361005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houde","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenzhen Engineering Laboratory of Geometry Measurement Techinology, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5688-929X","authenticated-orcid":false,"given":"Mingliang","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,6,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nrd1606","article-title":"In vivo drug discovery in the zebrafish","volume":"4","author":"Zon","year":"2005","journal-title":"Nat. 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[4th ed.]."}],"container-title":["Micromachines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-666X\/7\/6\/104\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:25:44Z","timestamp":1760210744000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-666X\/7\/6\/104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,20]]},"references-count":20,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2016,6]]}},"alternative-id":["mi7060104"],"URL":"https:\/\/doi.org\/10.3390\/mi7060104","relation":{},"ISSN":["2072-666X"],"issn-type":[{"type":"electronic","value":"2072-666X"}],"subject":[],"published":{"date-parts":[[2016,6,20]]}}}