{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T17:24:49Z","timestamp":1777310689322,"version":"3.51.4"},"reference-count":41,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T00:00:00Z","timestamp":1473120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI-1253293"],"award-info":[{"award-number":["DBI-1253293"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HG007233-01, R01-EB019453-01, DP2-AR068129-01"],"award-info":[{"award-number":["HG007233-01, R01-EB019453-01, DP2-AR068129-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Defence Advanced Research Projects Agency Living Foundries Program","award":["HR0011-12-C-0065, N66001-12-C-4211, HR0011-12-C-0066"],"award-info":[{"award-number":["HR0011-12-C-0065, N66001-12-C-4211, HR0011-12-C-0066"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Microfluidic devices are valuable for a variety of biotechnology applications, such as synthesizing biochemical libraries, screening enzymes, and analyzing single cells. However, normally, the devices are controlled using specialized pumps, which require expert knowledge to operate. Here, we demonstrate operation of poly(dimethylsiloxane) devices without pumps. We build a scaffold that holds the device and reagents to be infused in a format that can be inserted into a 50 mL falcon tube and spun in a common lab centrifuge. By controlling the device design and centrifuge spin speed, we infuse the reagents at controlled flow rates. We demonstrate the encapsulation and culture of clonal colonies of red and green Escherichia coli in droplets seeded from single cells.<\/jats:p>","DOI":"10.3390\/mi7090161","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T10:10:16Z","timestamp":1473156616000},"page":"161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Operation of Droplet-Microfluidic Devices with a Lab Centrifuge"],"prefix":"10.3390","volume":"7","author":[{"given":"Noorsher","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Bioengineering and Therapeutic Sciences, California Institute of Quantitative Biosciences (QB3), University of California, San Francisco, CA 94115, USA"}]},{"given":"David","family":"Sukovich","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Therapeutic Sciences, California Institute of Quantitative Biosciences (QB3), University of California, San Francisco, CA 94115, USA"}]},{"given":"Adam","family":"Abate","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Therapeutic Sciences, California Institute of Quantitative Biosciences (QB3), University of California, San Francisco, CA 94115, USA"}]}],"member":"1968","published-online":{"date-parts":[[2016,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.1039\/c001191f","article-title":"Dynamics of microfluidic droplets","volume":"16","author":"Baroud","year":"2010","journal-title":"Lab Chip"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"016601","DOI":"10.1088\/0034-4885\/75\/1\/016601","article-title":"Droplet based microfluidics","volume":"75","author":"Seemann","year":"2011","journal-title":"Rep. Prog. Phys."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5846","DOI":"10.1002\/anie.200906653","article-title":"Microdroplets in microfluidics: An evolving platform for discoveries in chemistry and biology","volume":"49","author":"Theberge","year":"2010","journal-title":"Angew. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.chembiol.2012.06.009","article-title":"Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution","volume":"19","author":"Kintses","year":"2012","journal-title":"Chem. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4004","DOI":"10.1073\/pnas.0910781107","article-title":"Ultrahigh-throughput screening in drop-based microfluidics for directed evolution","volume":"107","author":"Agresti","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Huebner, A., Srisa-Art, M., Holt, D., Abell, C., Hollfelder, F., deMello, A.J., and Edel, J. (2007). Quantitative detection of protein expression in single cells using droplet microfluidics. Chem. Commun., 1218\u20131220.","DOI":"10.1039\/b618570c"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1039\/c2lc21147e","article-title":"Droplet microfluidics for high-throughput biological assays","volume":"12","author":"Guo","year":"2012","journal-title":"Lab Chip"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"14195","DOI":"10.1073\/pnas.0903542106","article-title":"Droplet microfluidic technology for single-cell high-throughput screening","volume":"106","author":"Brouzes","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3563","DOI":"10.1002\/elps.200305584","article-title":"Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies","volume":"24","author":"Sia","year":"2003","journal-title":"Electrophoresis"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/(SICI)1522-2683(20000101)21:1<27::AID-ELPS27>3.0.CO;2-C","article-title":"Fabrication of microfluidic systems in poly(dimethylsiloxane)","volume":"21","author":"Anderson","year":"2000","journal-title":"Electrophoresis"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1039\/b308405a","article-title":"A flexible sample introduction method for polymer microfluidic chips using a push\/pull pressure pump","volume":"4","author":"Wu","year":"2004","journal-title":"Lab Chip"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1007\/s10404-005-0033-z","article-title":"Hydrodynamic focusing for vacuum-pumped microfluidics","volume":"1","author":"Stiles","year":"2005","journal-title":"Microfluid. Nanofluid."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"014107","DOI":"10.1063\/1.3567093","article-title":"Syringe-vacuum microfluidics: A portable technique to create monodisperse emulsions","volume":"5","author":"Abate","year":"2011","journal-title":"Biomicrofluidics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"095014","DOI":"10.1088\/0960-1317\/18\/9\/095014","article-title":"Using a circular groove surrounded inlet to generate monodisperse droplets inside a microfluidic chip in a gravity-driven manner","volume":"18","author":"Liu","year":"2008","journal-title":"J. Micromech. Microeng."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4669","DOI":"10.1021\/ac990682c","article-title":"Microfabricated Centrifugal Microfluidic Systems: Characterization and Multiple Enzymatic Assays","volume":"71","author":"Duffy","year":"1999","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"S103","DOI":"10.1088\/0960-1317\/17\/7\/S07","article-title":"The centrifugal microfluidic Bio-Disk platform","volume":"17","author":"Haeberle","year":"2007","journal-title":"J. Micromech. Microeng."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Hoffmann, J., Mark, D., Zengerle, R., and von Stetten, F. (2009, January 21\u201325). Liquid reagent storage and release for centrifuge operated LAB-on-a-chip systems based on a burstable seal. Proceedings of the 15th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers\u201909), Denver, CO, USA.","DOI":"10.1109\/SENSOR.2009.5285652"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1039\/C5LC01580D","article-title":"LabDisk for SAXS: A centrifugal microfluidic sample preparation platform for small-angle X-ray scattering","volume":"16","author":"Schwemmer","year":"2016","journal-title":"Lab Chip"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1002\/elps.200600351","article-title":"CE chips fabricated by injection molding and polyethylene\/thermoplastic elastomer film packaging methods","volume":"28","author":"Huang","year":"2007","journal-title":"Electrophoresis"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0924-4247(00)00296-X","article-title":"Hot embossing as a method for the fabrication of polymer high aspect ratio structures","volume":"83","author":"Becker","year":"2000","journal-title":"Sens. Actuators A Phys."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"429","DOI":"10.2144\/05383RV02","article-title":"Disposable microfluidic devices: fabrication, function, and application","volume":"38","author":"Fiorini","year":"2005","journal-title":"BioTechniques"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1002\/ange.200390172","article-title":"A Microfluidic System for Controlling Reaction Networks in Time","volume":"115","author":"Song","year":"2003","journal-title":"Angew. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1016\/j.addr.2013.05.009","article-title":"Multiphase flow microfluidics for the production of single or multiple emulsions for drug delivery","volume":"65","author":"Zhao","year":"2013","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s10404-006-0134-3","article-title":"Flow rate effect on droplet control in a co-flowing microfluidic device","volume":"3","author":"Hong","year":"2007","journal-title":"Microfluid. Nanofluid."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7336","DOI":"10.1002\/anie.200601554","article-title":"Reactions in droplets in microfluidic channels","volume":"45","author":"Song","year":"2006","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/S1369-7021(08)70053-1","article-title":"Designer emulsions using microfluidics","volume":"11","author":"Shah","year":"2008","journal-title":"Mater. Today"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8970","DOI":"10.1002\/anie.200701358","article-title":"Controllable monodisperse multiple emulsions","volume":"46","author":"Chu","year":"2007","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1039\/c2lc21164e","article-title":"Microfluidic synthesis of advanced microparticles for encapsulation and controlled release","volume":"12","author":"Duncanson","year":"2012","journal-title":"Lab Chip"},{"key":"ref_29","first-page":"1","article-title":"Soft Lithography for Microfluidics: A Review","volume":"2","author":"Kim","year":"2008","journal-title":"Biochip J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1039\/b800001h","article-title":"Glass coating for PDMS microfluidic channels by sol-gel methods","volume":"8","author":"Abate","year":"2008","journal-title":"Lab Chip"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1039\/c3lc51176f","article-title":"Syringe-pump-induced fluctuation in all-aqueous microfluidic system implications for flow rate accuracy","volume":"14","author":"Li","year":"2014","journal-title":"Lab Chip"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1039\/b510841a","article-title":"Formation of droplets and bubbles in a microfluidic T-junction\u2014scaling and mechanism of break-up","volume":"6","author":"Garstecki","year":"2006","journal-title":"Lab Chip"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5130","DOI":"10.1039\/c2lc40938k","article-title":"Flow focusing geometry generates droplets through a plug and squeeze mechanism","volume":"12","author":"Romero","year":"2012","journal-title":"Lab Chip"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.compfluid.2014.05.023","article-title":"Droplet formation via squeezing mechanism in a microfluidic flow-focusing device","volume":"100","author":"Gupta","year":"2014","journal-title":"Comput. Fluids"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.snb.2014.02.042","article-title":"Generation of water-in-oil and oil-in-water microdroplets in polyester-toner microfluidic devices","volume":"196","author":"Piccin","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1039\/b805456h","article-title":"Controlled encapsulation of single cells into monodisperse picoliter drops","volume":"8","author":"Edd","year":"2008","journal-title":"Lab Chip"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1039\/b802941e","article-title":"Drop-based microfluidic devices for encapsulation of single cells","volume":"8","author":"Duan","year":"2008","journal-title":"Lab Chip"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3439","DOI":"10.1039\/C5LC00614G","article-title":"The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation","volume":"15","author":"Collins","year":"2015","journal-title":"Lab Chip"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.chembiol.2008.04.004","article-title":"Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms","volume":"15","author":"Lieber","year":"2008","journal-title":"Chem. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1021\/cb100336p","article-title":"Encapsulating Bacteria in Agarose Microparticles Using Microfluidics for High-Throughput Cell Analysis Isolation","volume":"6","author":"Eun","year":"2011","journal-title":"ACS Chem. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"12176","DOI":"10.1002\/anie.201200460","article-title":"Droplet microfluidics\u2014A tool for single-cell analysis","volume":"51","author":"Joensson","year":"2012","journal-title":"Angew. 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