{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T16:03:18Z","timestamp":1760889798565,"version":"build-2065373602"},"reference-count":29,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T00:00:00Z","timestamp":1550620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007633","name":"Stiftelsen f\u00f6r Milj\u00f6strategisk Forskning","doi-asserted-by":"publisher","award":["MI11.02"],"award-info":[{"award-number":["MI11.02"]}],"id":[{"id":"10.13039\/100007633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Remote Sensing"],"abstract":"<jats:p>Using light emitting diodes (LEDs) for greenhouse illumination enables the use of automatic control, since both light quality and quantity can be tuned. Potential candidate signals when using biological feedback for light optimisation are steady-state chlorophyll a fluorescence gains at 740 nm, defined as the difference in steady-state fluorescence at 740 nm divided by the difference in incident light quanta caused by (a small) excitation of different LED colours. In this study, experiments were conducted under various background light (quality and quantity) to evaluate if these fluorescence gains change relative to each other. The light regimes investigated were intensities in the range 160\u20131000     \u03bc mol \u00a0  m  \u2212 2   \u00a0  s  \u2212 1      , and a spectral distribution ranging from 50% to 100% red light. No significant changes in the mutual relation of the fluorescence gains for the investigated LED colours (400, 420, 450, 530, 630 and 660 nm), could be observed when the background light quality was changed. However, changes were noticed as function of light quantity. When passing the photosynthesis saturate intensity level, no further changes in the mutual fluorescence gains could be observed.<\/jats:p>","DOI":"10.3390\/rs11040434","type":"journal-article","created":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T11:45:39Z","timestamp":1550663139000},"page":"434","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Relation between Changes in Photosynthetic Rate and Changes in Canopy Level Chlorophyll Fluorescence Generated by Light Excitation of Different Led Colours in Various Background Light"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1061-7958","authenticated-orcid":false,"given":"Linn\u00e9a","family":"Ahlman","sequence":"first","affiliation":[{"name":"Department of Electrical Engineering, Chalmers University of Technology, SE-41293 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"B\u00e5nkestad","sequence":"additional","affiliation":[{"name":"Heliospectra AB, SE-41458 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torsten","family":"Wik","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, Chalmers University of Technology, SE-41293 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,20]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Advances in greenhouse automation and controlled environment agriculture: A transition to plant factories and urban agriculture","volume":"11","author":"Shamshiri","year":"2018","journal-title":"Int. J. Agric. Biol. Eng."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Durner, E.F. (2013). Principles of Horticultural Physiology, CAB International.","DOI":"10.1079\/9781780643069.0000"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"223","DOI":"10.23986\/afsci.7897","article-title":"The effects of light-emitting diode lighting on greenhouse plant growth and quality","volume":"22","author":"Olle","year":"2013","journal-title":"Agric. Food Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.21273\/HORTSCI.50.8.1128","article-title":"Spectral Effects of Artificial Light on Plant Physiology and Secondary Metabolism: A Review","volume":"50","author":"Ouzounis","year":"2015","journal-title":"HortScience"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Snowden, M.C., Cope, K.R., and Bugbee, B. (2016). Sensitivity of Seven Diverse Species to Blue and Green Light: Interactions with Photon Flux. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0163121"},{"key":"ref_6","first-page":"1","article-title":"The dependence of carbon dioxide assimilation in a higher plant on wavelength of radiation","volume":"95","author":"Hoover","year":"1937","journal-title":"Smithson. Inst. Misc. Coll."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0002-1571(71)90022-7","article-title":"Action Spectrum, Absorptance and Quantum Yield of Photosynthesis in Crop Plants","volume":"9","author":"McCree","year":"1972","journal-title":"Agric. Meteorol."},{"key":"ref_8","first-page":"355","article-title":"Action spectra for photosynthesis in higher plants","volume":"17","author":"Inada","year":"1976","journal-title":"Plant Cell Physiol."},{"key":"ref_9","first-page":"746","article-title":"Dependence of yield of photosynthesis in long-wave red on wavelength and intensity of supplementary light","volume":"125","author":"Emerson","year":"1957","journal-title":"Science"},{"key":"ref_10","unstructured":"Wik, T., Carstensen, A., and Pocock, T. (2012). Spectrum Optimization for Artificial Illumination. (PCT\/EP2013\/069,820), WO Patent."},{"key":"ref_11","unstructured":"Papageorgiou, G.C. (2004). Pulse-Amplitude-Modulation (PAM) Fluorometry and Saturation Pulse Method: An Overview. Chlorophyll a Fluorescence: A Signature of Photosynthesis, Springer."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/978-1-4020-3218-9_12","article-title":"Analysis of the Chlorophyll a Fluorescence Transient","volume":"Volume 19","author":"Papageorgiou","year":"2004","journal-title":"Chlorophyll a Fluorescence"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1002\/andp.18601850205","article-title":"Ueber das Verh\u00e4ltniss zwischen dem Emissionsverm\u00f6gen und dem Absorptionsverm\u00f6gen der K\u00f6rper f\u00fcr W\u00e4rme und Licht","volume":"185","author":"Kirchhoff","year":"1860","journal-title":"Ann. Phys."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.rse.2004.02.012","article-title":"A new instrument for passive remote sensing1. Measurements of sunlight-induced chlorophyll fluorescence","volume":"91","author":"Moya","year":"2004","journal-title":"Remote Sens. Environ."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4065","DOI":"10.1093\/jxb\/eru191","article-title":"Linking chlorophyll a fluorescence to photosynthesis for remote sensing applications: Mechanisms and challenges","volume":"65","author":"Tyystjarvi","year":"2014","journal-title":"J. Exp. Bot."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.compag.2017.07.023","article-title":"Using chlorophyll a fluorescence gains to optimize LED light spectrum for short term photosynthesis","volume":"142","author":"Ahlman","year":"2017","journal-title":"Comput. Electron. Agric."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"367","DOI":"10.17660\/ActaHortic.2016.1134.48","article-title":"LED spectrum optimisation using steady-state fluorescence gains","volume":"1134","author":"Ahlman","year":"2016","journal-title":"Acta Hortic."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1034\/j.1399-3054.2002.1140209.x","article-title":"Steady-state chlorophyll fluorescence (Fs) measurements as a tool to follow variations of net CO2 assimilation and stomatal conductance during water-stress in C3 plants","volume":"114","author":"Flexas","year":"2002","journal-title":"Physiol. Plant."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"E2511","DOI":"10.1073\/pnas.1406996111","article-title":"Reply to Magnani et al.: Linking large-scale chlorophyll fluorescence observations with cropland gross primary production","volume":"111","author":"Guanter","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1093\/jexbot\/51.345.659","article-title":"Chlorophyll fluorescence\u2014A practical guide","volume":"51","author":"Maxwell","year":"2000","journal-title":"J. Exp. Bot."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.agrformet.2008.07.007","article-title":"A model for chlorophyll fluorescence and photosynthesis at leaf scale","volume":"149","author":"Verhoef","year":"2009","journal-title":"Agric. For. Meteorol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2312","DOI":"10.1002\/2014JG002713","article-title":"Models of fluorescence and photosynthesis for interpreting measurements of solar-induced chlorophyll fluorescence","volume":"119","author":"Berry","year":"2014","journal-title":"J. Geophys. Res. Biogeosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4488","DOI":"10.1364\/AO.43.004488","article-title":"Dualex: A new instrument for field measurements of epidermal ultraviolet absorbance by chlorophyll fluorescence","volume":"43","author":"Goulas","year":"2004","journal-title":"Appl. Opt."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s13593-011-0041-1","article-title":"Sensing crop nitrogen status with fluorescence indicators. A review","volume":"32","author":"Tremblay","year":"2012","journal-title":"Agron. Sustain. Dev."},{"key":"ref_25","unstructured":"Box, G., Hunter, W.G., and Hunter, J.S. (1978). Statistics for Experimenters, John Wiley & Sons."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.compag.2016.06.002","article-title":"Remote detection of light tolerance in Basil through frequency and transient analysis of light induced fluorescence","volume":"127","author":"Carstensen","year":"2016","journal-title":"Comput. Electron. Agric."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.jplph.2017.08.009","article-title":"In situ optical properties of foliar flavonoids: Implication for non-destructive estimation of flavonoid content","volume":"218","author":"Gitelson","year":"2017","journal-title":"J. Plant Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8106","DOI":"10.1021\/acs.jafc.5b02842","article-title":"Flavonoids Affect the Light Reaction of Photosynthesis in Vitro and in Vivo as Well as the Growth of Plants","volume":"63","year":"2015","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","unstructured":"Lawlor, D.W. (2001). Photosynthesis, Bios Scientific Publishers Ltd.. [3rd ed.]."}],"container-title":["Remote Sensing"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-4292\/11\/4\/434\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:33:24Z","timestamp":1760186004000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-4292\/11\/4\/434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,20]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,2]]}},"alternative-id":["rs11040434"],"URL":"https:\/\/doi.org\/10.3390\/rs11040434","relation":{},"ISSN":["2072-4292"],"issn-type":[{"type":"electronic","value":"2072-4292"}],"subject":[],"published":{"date-parts":[[2019,2,20]]}}}