{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T13:19:03Z","timestamp":1773667143647,"version":"3.50.1"},"reference-count":50,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T00:00:00Z","timestamp":1554336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["17-76-20032"],"award-info":[{"award-number":["17-76-20032"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Remote Sensing"],"abstract":"<jats:p>The photochemical reflectance index (PRI) is a widely used spectral index which can show stress-induced changes in photosynthesis (e.g., increase of the nonphotochemical quenching of chlorophyll fluorescence (NPQ)). The artificial illumination of plants improves the efficiency of estimation of photosynthetic processes on the basis of PRI measurements. However, the simultaneous activity of different light sources with different locations can disturb the measurement of PRI. Using pulses of a green-yellow measuring light can potentially solve this problem. The aim of the present work was to investigate the possibility of using green-yellow light pulses for the investigation of light-induced changes in PRI in higher plants (pea, wheat, and pumpkin) and for the analysis of connection between PRI and the energy-dependent component of NPQ (NPQF). First, we showed that using green-yellow light pulses eliminated shifts of reflected light, which were connected with the application of a red actinic light. Second, analysis of light dependences of NPQF, the absolute value of PRI, and changes in PRI (the difference between the PRI under the actinic light and the initial value of PRI without this light, \u0394PRI) showed that the dynamics of the increase of NPQF and the decrease of PRI and \u0394PRI were similar. Changes in NPQF and \u0394PRI were found to be significant. In contrast, changes in the absolute value of PRI were not significant in most of the variants of the experiments. Third, scatter plots between NPQF and \u0394PRI showed similar linear correlations for investigated species; moreover, a total set of experimental points (for pea, wheat, and pumpkin) were also described by the same linear regression. Thus, our results show that (i) pulses of green-yellow measuring light can be used for measurements of PRI, and (ii) \u0394PRI is a more effective indicator for the estimation of NPQ than the absolute value of PRI.<\/jats:p>","DOI":"10.3390\/rs11070810","type":"journal-article","created":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T11:31:57Z","timestamp":1554377517000},"page":"810","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Analysis of Light-Induced Changes in the Photochemical Reflectance Index (PRI) in Leaves of Pea, Wheat, and Pumpkin Using Pulses of Green-Yellow Measuring Light"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2918-8134","authenticated-orcid":false,"given":"Ekaterina","family":"Sukhova","sequence":"first","affiliation":[{"name":"Department of Biophysics, N.I. Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8712-9127","authenticated-orcid":false,"given":"Vladimir","family":"Sukhov","sequence":"additional","affiliation":[{"name":"Department of Biophysics, N.I. Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1104\/pp.15.01935","article-title":"Nonphotochemical chlorophyll fluorescence quenching: Mechanism and effectiveness in protecting plants from photodamage","volume":"170","author":"Ruban","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4065","DOI":"10.1093\/jxb\/eru191","article-title":"Linking chlorophyll a fluorescence to photosynthesis for remote sensing applications: Mechanisms and challenges","volume":"65","author":"Atherton","year":"2014","journal-title":"J. Exp. 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