{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T15:59:08Z","timestamp":1778169548898,"version":"3.51.4"},"reference-count":47,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T00:00:00Z","timestamp":1603238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Seventh Framework Programme","award":["311929"],"award-info":[{"award-number":["311929"]}]},{"DOI":"10.13039\/501100009880","name":"Regione Lazio","doi-asserted-by":"publisher","award":["FILAS-RU-2014-1191"],"award-info":[{"award-number":["FILAS-RU-2014-1191"]}],"id":[{"id":"10.13039\/501100009880","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["Rientro dei cervelli"],"award-info":[{"award-number":["Rientro dei cervelli"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Remote Sensing"],"abstract":"<jats:p>Replacing fossil fuels with cellulosic biofuels is a valuable component of reducing the drivers of climate change. This leads to a requirement to develop more productive bioenergy crops, such as Arundo donax with the aim of increasing above-ground biomass (AGB). However, direct measurement of AGB is time consuming, destructive, and labor-intensive. Phenotyping of plant height and biomass production is a bottleneck in genomics- and phenomics-assisted breeding. Here, an unmanned aerial vehicle (UAV) for remote sensing equipped with light detection and ranging (LiDAR) was tested for remote plant height and biomass determination in A. donax. Experiments were conducted on three A. donax ecotypes grown in well-watered and moderate drought stress conditions. A novel UAV-LiDAR data collection and processing workflow produced a dense three-dimensional (3D) point cloud for crop height estimation through a normalized digital surface model (DSM) that acts as a crop height model (CHM). Manual measurements of crop height and biomass were taken in parallel and compared to LiDAR CHM estimates. Stepwise multiple regression was used to estimate biomass. Analysis of variance (ANOVA) tests and pairwise comparisons were used to determine differences between ecotypes and drought stress treatments. We found a significant relationship between the sensor readings and manually measured crop height and biomass, with determination coefficients of 0.73 and 0.71 for height and biomass, respectively. Differences in crop heights were detected more precisely from LiDAR estimates than from manual measurement. Crop biomass differences were also more evident in LiDAR estimates, suggesting differences in ecotypes\u2019 productivity and tolerance to drought. Based on these results, application of the presented UAV-LiDAR workflow will provide new opportunities in assessing bioenergy crop morpho-physiological traits and in delivering improved genotypes for biorefining.<\/jats:p>","DOI":"10.3390\/rs12203464","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T20:51:00Z","timestamp":1603399860000},"page":"3464","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["UAV-Based LiDAR for High-Throughput Determination of Plant Height and Above-Ground Biomass of the Bioenergy Grass Arundo donax"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4325-951X","authenticated-orcid":false,"given":"Mauro","family":"Maesano","sequence":"first","affiliation":[{"name":"Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1980-5365","authenticated-orcid":false,"given":"Sacha","family":"Khoury","sequence":"additional","affiliation":[{"name":"Forest Ecology and Conservation Group, Department of Plant Sciences, University of Cambridge Conservation Research Institute, The David Attenborough Building, Cambridge CB2 3QZ, UK"}]},{"given":"Farid","family":"Nakhle","sequence":"additional","affiliation":[{"name":"Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy"}]},{"given":"Andrea","family":"Firrincieli","sequence":"additional","affiliation":[{"name":"Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy"},{"name":"Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy"}]},{"given":"Alan","family":"Gay","sequence":"additional","affiliation":[{"name":"Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Aberystwyth SY23 3EE, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5176-3492","authenticated-orcid":false,"given":"Flavia","family":"Tauro","sequence":"additional","affiliation":[{"name":"Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy"}]},{"given":"Antoine","family":"Harfouche","sequence":"additional","affiliation":[{"name":"Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.3389\/fpls.2017.01111","article-title":"Unmanned aerial vehicle remote sensing for field-based crop phenotyping: Current status and perspectives","volume":"8","author":"Yang","year":"2017","journal-title":"Front. 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