{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T13:17:22Z","timestamp":1781529442199,"version":"3.54.1"},"reference-count":38,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41471277"],"award-info":[{"award-number":["41471277"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY20D010004"],"award-info":[{"award-number":["LY20D010004"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ20F050006"],"award-info":[{"award-number":["LQ20F050006"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010248","name":"Zhejiang Province Public Welfare Technology Application Research Project","doi-asserted-by":"publisher","award":["LGN18F030002"],"award-info":[{"award-number":["LGN18F030002"]}],"id":[{"id":"10.13039\/501100010248","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Major Agricultural Technology Cooperative Popularization Project of Zhejiang Province of China","award":["2020XTTGCY01-02"],"award-info":[{"award-number":["2020XTTGCY01-02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Remote Sensing"],"abstract":"<jats:p>The in vivo features of the absorption of leaf photosynthetic and photo-protective pigments are closely linked to the leaf spectrum in the 400\u2013800 nm regions. However, this information is difficult to obtain because the overlapping leaf pigments can mask the contribution of individual pigments to the leaf spectrum. Here, to limit the masking phenomenon between these pigments, the separation technology for leaf spectral overlapping was employed in the PROSPECT model with the ZJU dataset. The main results of this study include the following aspects: (1) the absorption coefficients of separated chlorophyll a and b, carotenoids and anthocyanins in the leaf in vivo display the physical principles of forming an absorption spectrum similar to those in an organic solution; (2) the differences in the position of each absorption peak of pigments between the leaf in vivo and in an organic solution can be described by a spectral displacement parameter; and (3) the overlapping characteristics between the separated pigments in the leaf in vivo are clearly drawn by a range of absorption feature (RAF) parameter. Moreover, the absorption coefficients of the separated pigments were successfully applied in leaf spectral modeling and pigment retrieval. The results show that the separated multiple pigment absorption coefficients from the leaf spectrum in vivo are effective and provide a framework for future refinements in describing leaf optical properties.<\/jats:p>","DOI":"10.3390\/rs12213615","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T20:00:29Z","timestamp":1604433629000},"page":"3615","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Exploring the Optical Properties of Leaf Photosynthetic and Photo-Protective Pigments In Vivo Based on the Separation of Spectral Overlapping"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6865-8656","authenticated-orcid":false,"given":"Yao","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Artificial Intelligence, Hangzhou Dianzi University, Hangzhou 310018, China"},{"name":"Institute of Agricultural Remote Sensing and Information Application, Zijingang Campus, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Agricultural Remote Sensing and Information System, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengjie","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Artificial Intelligence, Hangzhou Dianzi University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4627-6021","authenticated-orcid":false,"given":"Jingfeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Remote Sensing and Information Application, Zijingang Campus, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Agricultural Remote Sensing and Information System, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumin","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Remote Sensing and Information Application, Zijingang Campus, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Agricultural Remote Sensing and Information System, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0226-8492","authenticated-orcid":false,"given":"Ran","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Artificial Intelligence, Hangzhou Dianzi University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1361-0663","authenticated-orcid":false,"given":"Hongze","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Artificial Intelligence, Hangzhou Dianzi University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengnong","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Artificial Intelligence, Hangzhou Dianzi University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaihua","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Artificial Intelligence, Hangzhou Dianzi University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S0034-4257(02)00010-X","article-title":"Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stages","volume":"81","author":"Sims","year":"2002","journal-title":"Remote Sens. 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