{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T17:24:20Z","timestamp":1764350660112,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Remote Sensing"],"abstract":"<jats:p>This study introduces a semi-empirical algorithm to estimate the extent of the phycocyanin (PC) concentration in eutrophic freshwater bodies; this is achieved by studying the reflectance characteristics of the red and near-red spectral regions, especially the shifting of the peak near 700 nm towards longer wavelengths. Spectral measurements in a darkroom environment over the pure-cultured cyanobacteria Microcystis showed that the shift is proportional to the algal biomass. A similar proportional trend was found from extensive field measurement data. The data also showed that the correlation of the magnitude of the shift with the PC concentration was greater than that with chlorophyll-a. This indicates that the characteristic can be a useful index to quantify cyanobacterial biomass. Based on these observations, a new PC algorithm was proposed that uses the remote sensing reflectance of the peak band around 700 nm and the trough band around 620 nm, and the magnitude of the peak shift near 700 nm. The efficacy of the algorithm was tested with 300 sets of field data, and the results were compared to select algorithms for the PC concentration prediction. The new algorithm performed better than the other algorithms with respect to most error indices, especially the mean relative error, indicating that the algorithm can reduce errors when PC concentrations are low. The algorithm was also applied to a hyperspectral dataset obtained through aerial imaging, in order to predict the spatial distribution of the PC concentration in an approximately 86 km long reach of the Nakdong River.<\/jats:p>","DOI":"10.3390\/rs13163335","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T10:24:17Z","timestamp":1629714257000},"page":"3335","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Quantification of Phycocyanin in Inland Waters through Remote Measurement of Ratios and Shifts in Reflection Spectral Peaks"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6685-2498","authenticated-orcid":false,"given":"Gibeom","family":"Nam","sequence":"first","affiliation":[{"name":"The National Institute of Environmental Research, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3937-0562","authenticated-orcid":false,"given":"Hyunjoo","family":"Shin","sequence":"additional","affiliation":[{"name":"The National Institute of Environmental Research, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rim","family":"Ha","sequence":"additional","affiliation":[{"name":"The Seoul Institute of Technology, 37 Maebongsan-ro, Mapo-gu, Seoul 03909, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyunoh","family":"Song","sequence":"additional","affiliation":[{"name":"The National Institute of Environmental Research, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaehyun","family":"Yoo","sequence":"additional","affiliation":[{"name":"The National Institute of Environmental Research, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyuk","family":"Lee","sequence":"additional","affiliation":[{"name":"The National Institute of Environmental Research, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanghyun","family":"Park","sequence":"additional","affiliation":[{"name":"The National Institute of Environmental Research, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taegu","family":"Kang","sequence":"additional","affiliation":[{"name":"The National Institute of Environmental Research, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2255-0965","authenticated-orcid":false,"given":"Kyunghyun","family":"Kim","sequence":"additional","affiliation":[{"name":"The National Institute of Environmental Research, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1080\/14634980802099604","article-title":"Influence of environmental conditions on late-summer cyanobacterial abundance in Saginaw Bay, Lake Huron","volume":"11","author":"Millie","year":"2008","journal-title":"Aquat. 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