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With the rapid development of drone technology, the estimation of crop LAI based on drone imagery and vegetation indices is becoming increasingly popular. However, there is still a lack of detailed research on the feasibility of using image texture to estimate LAI and the impact of combining texture indices with vegetation indices on LAI estimation accuracy. In this study, two key growth stages of winter wheat (i.e., the stages of green-up and jointing) were selected, and LAI was calculated using digital hemispherical photography. The feasibility of predicting winter wheat LAI was explored under three conditions: vegetation index, texture index, and a combination of vegetation index and texture index, at flight heights of 20 m and 40 m. Two feature selection methods (Lasso and recursive feature elimination) were combined with four machine learning regression models (multiple linear regression, random forest, support vector machine, and backpropagation neural network). The results showed that during the vegetative growth stage of winter wheat, the model combining texture information with vegetation indices performed better than the models using vegetation indices alone or texture information alone. Among them, the best prediction result based on vegetation index was RFECV-MLR at a flight height of 40 m (R2 = 0.8943, RMSE = 0.4139, RRMSE = 0.1304, RPD = 3.0763); the best prediction result based on texture index was RFECV-RF at a flight height of 40 m (R2 = 0.8894, RMSE = 0.4236, RRMSE = 0.1335, RPD = 3.0063); and the best prediction result combining texture and index was RFECV-RF at a flight height of 40 m (R2 = 0.9210, RMSE = 0.3579, RRMSE = 0.1128, RPD = 3.5575). The results of this study demonstrate that combining vegetation indices and texture from multispectral drone imagery can improve the accuracy of LAI estimation during the vegetative growth stage of winter wheat. In addition, selecting a flight height of 40 m can improve efficiency in large-scale agricultural field monitoring, as this study showed that drone data at flight heights of 20 m and 40 m did not significantly affect model accuracy.<\/jats:p>","DOI":"10.3390\/rs15245715","type":"journal-article","created":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T08:55:16Z","timestamp":1702457716000},"page":"5715","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Combining Texture, Color, and Vegetation Index from Unmanned Aerial Vehicle Multispectral Images to Estimate Winter Wheat Leaf Area Index during the Vegetative Growth Stage"],"prefix":"10.3390","volume":"15","author":[{"given":"Weilong","family":"Li","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genetics and Physiology\/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8322-1316","authenticated-orcid":false,"given":"Jianjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genetics and Physiology\/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genetics and Physiology\/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1112-6676","authenticated-orcid":false,"given":"Quan","family":"Yin","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genetics and Physiology\/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiling","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genetics and Physiology\/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guisheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Joint International Research Laboratory of Agriculture and Agricultural Product Safety, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyang","family":"Huo","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genetics and Physiology\/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1093\/oxfordjournals.aob.a083165","article-title":"Comparative Physiological Studies on the Growth of Field Crops: II. 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