{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:40:58Z","timestamp":1760222458572,"version":"build-2065373602"},"reference-count":22,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2009,12,30]],"date-time":"2009-12-30T00:00:00Z","timestamp":1262131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The direct and accurate estimation of nitric dioxide levels is an extremely laborious and technically demanding procedure in the molecular diagnostics of inflammatory processes. The aim of this work is to demonstrate that a stop-flow technique utilizing a specific spectroscopic biosensor can be used for detection of nanomolar quantities of NO2 in biological milieu. The use of novel compound cis-[Cr(C2O4)(AaraNH2)(OH2)2]+ increases NO2 estimation accuracy by slowing down the rate of NO2 uptake. In this study, an animal model of pancreatitis, where nitrosative stress is induced by either 3g\/kg bw or 1.5 g\/kg bw dose of L-arginine, was used. Biochemical parameters and morphological characteristics of acute pancreatitis were monitored, specifically assessing pancreatic acinar cell death mode, NO2 generation and cellular glutathione level. The severity of the process correlated positively with NO2 levels in pancreatic acinar cell cytosol samples, and negatively with cellular glutathione levels.<\/jats:p>","DOI":"10.3390\/s100100280","type":"journal-article","created":{"date-parts":[[2009,12,31]],"date-time":"2009-12-31T02:45:58Z","timestamp":1262227558000},"page":"280-291","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat"],"prefix":"10.3390","volume":"10","author":[{"given":"Dagmara","family":"Jacewicz","sequence":"first","affiliation":[{"name":"Department of General and Inorganic Chemistry, University of Gda\u0144sk, Sobieskiego 18\/19, 80-952 Gda\u0144sk, Poland"}]},{"given":"Aleksandra","family":"Dabrowska","sequence":"additional","affiliation":[{"name":"Department of General and Inorganic Chemistry, University of Gda\u0144sk, Sobieskiego 18\/19, 80-952 Gda\u0144sk, Poland"}]},{"given":"Dariusz","family":"Wyrzykowski","sequence":"additional","affiliation":[{"name":"Department of General and Inorganic Chemistry, University of Gda\u0144sk, Sobieskiego 18\/19, 80-952 Gda\u0144sk, Poland"}]},{"given":"Joanna","family":"Pranczk","sequence":"additional","affiliation":[{"name":"Department of General and Inorganic Chemistry, University of Gda\u0144sk, Sobieskiego 18\/19, 80-952 Gda\u0144sk, Poland"}]},{"given":"Michal","family":"Wozniak","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Medical University of Gda\u0144sk, D\u0119binki 1, 80-211, Gda\u0144sk, Poland"}]},{"given":"Jolanta","family":"Kubasik-Juraniec","sequence":"additional","affiliation":[{"name":"Department of Electron Microscopy, Medical University of Gda\u0144sk, D\u0119binki 1, 80-211, Gda\u0144sk, Poland"}]},{"given":"Narcyz","family":"Knap","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Medical University of Gda\u0144sk, D\u0119binki 1, 80-211, Gda\u0144sk, Poland"}]},{"given":"Kamila","family":"Siedlecka","sequence":"additional","affiliation":[{"name":"Department of Histology & Immunology, Medical University of Gda\u0144sk, D\u0119binki 1, 80-211, Gda\u0144sk, Poland"}]},{"given":"Alexander J.","family":"Neuwelt","sequence":"additional","affiliation":[{"name":"Department of Neurology, Oregon Health & Sciences University, Portland OR 97239, USA"}]},{"given":"Lech","family":"Chmurzynski","sequence":"additional","affiliation":[{"name":"Department of General and Inorganic Chemistry, University of Gda\u0144sk, Sobieskiego 18\/19, 80-952 Gda\u0144sk, Poland"}]}],"member":"1968","published-online":{"date-parts":[[2009,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1016\/S0024-3205(02)01971-9","article-title":"Effects of resveratrol-related hydroxystilbenes on the nitric oxide production in macrophage cells: structural requirements and mechanism of action","volume":"71","author":"Cho","year":"2002","journal-title":"Life Sci"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1097\/00006676-200003000-00006","article-title":"Experimental pancreatitis induced by synthetic prooxidant tert-butyl hydroperoxide","volume":"20","author":"Sledzinski","year":"2000","journal-title":"Pancreas"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/S0039-6060(95)80008-5","article-title":"Interleukin-1 receptor antagonist decreases severity of experimental acute pancreatitis","volume":"117","author":"Norman","year":"1995","journal-title":"Surgery"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1136\/gut.33.9.1264","article-title":"Role of interleukin-6 in acute pancreatitis. 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