{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T16:28:02Z","timestamp":1776356882426,"version":"3.51.2"},"reference-count":73,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2010,1,21]],"date-time":"2010-01-21T00:00:00Z","timestamp":1264032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this work, a novel voltammetric ethanol biosensor was constructed using alcohol dehydrogenase (ADH). Firstly, alcohol dehydrogenase was immobilized on the surface of a glassy carbon electrode modified by cellulose acetate (CA) bonded to toluidine blue O (TBO). Secondly, the surface was covered by a glutaraldehyde\/bovine serum albumin (BSA) cross-linking procedure to provide a new voltammetric sensor for the ethanol determination. In order to fabricate the biosensor, a new electrode matrix containing insoluble Toluidine Blue O (TBO) was obtained from the process, and enzyme\/coenzyme was combined on the biosensor surface. The influence of various experimental conditions was examined for the characterization of the optimum analytical performance. The developed biosensor exhibited sensitive and selective determination of ethanol and showed a linear response between 1 \u00d7 10\u22125 M and 4 \u00d7 10\u22124 M ethanol. A detection limit calculated as three times the signal-to-noise ratio was 5.0 \u00d7 10\u22126 M. At the end of the 20th day, the biosensor still retained 50% of its initial activity.<\/jats:p>","DOI":"10.3390\/s100100748","type":"journal-article","created":{"date-parts":[[2010,1,21]],"date-time":"2010-01-21T08:53:33Z","timestamp":1264064013000},"page":"748-764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Development of an Alcohol Dehydrogenase Biosensor for Ethanol Determination with Toluidine Blue O Covalently Attached to a Cellulose Acetate Modified Electrode"],"prefix":"10.3390","volume":"10","author":[{"given":"\u015eenol","family":"Alpat","sequence":"first","affiliation":[{"name":"Department of Chemistry Education, Dokuz Eylul University, 35150 Buca-Izmir, Turkey"}]},{"given":"Azmi","family":"Telefoncu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Ege University, 35100 Bornova-Izmir, Turkey"}]}],"member":"1968","published-online":{"date-parts":[[2010,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0003-2670(99)00135-X","article-title":"Determination of breath alcohol using a differential-type amperometric biosensor based on alcohol dehydrogenase","volume":"390","author":"Park","year":"1999","journal-title":"Anal. 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