{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:35:54Z","timestamp":1760708154009,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2010,1,22]],"date-time":"2010-01-22T00:00:00Z","timestamp":1264118400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Fluorescence immunoassays employing monoclonal antibodies directed against the explosive 2,4,6-trinitrotoluene (TNT) were conducted in a multi-channel microimmunosensor. The multi-channel microimmunosensor was prepared in poly (methyl methacrylate) (PMMA) via hot embossing from a brass molding tool. The multi-channeled microfluidic device was sol-gel coated to generate a siloxane surface that provided a scaffold for antibody immobilization. AlexaFluor-cadaverine-trinitrobenzene (AlexaFluor-Cad-TNB) was used as the reporter molecule in a displacement immunoassay. The limit of detection was 1-10 ng\/mL (ppb) with a linear dynamic range that covered three orders of magnitude. In addition, antibody crossreactivity was investigated using hexahydro-1,3,5-triazine (RDX), HMX, 2,4-dinitrotoluene (DNT), 4-nitrotoluene (4-NT) and 2-amino-4,6-DNT.<\/jats:p>","DOI":"10.3390\/s100100876","type":"journal-article","created":{"date-parts":[[2010,1,25]],"date-time":"2010-01-25T02:53:55Z","timestamp":1264388035000},"page":"876-889","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Fluorescence-based Sensing of 2,4,6-Trinitrotoluene (TNT) Using a Multi-channeled Poly(methyl methacrylate) (PMMA) Microimmunosensor"],"prefix":"10.3390","volume":"10","author":[{"given":"Paul T.","family":"Charles","sequence":"first","affiliation":[{"name":"Center for Bio\/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA"}]},{"given":"Andre A.","family":"Adams","sequence":"additional","affiliation":[{"name":"Center for Bio\/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA"}]},{"given":"Peter B.","family":"Howell","sequence":"additional","affiliation":[{"name":"Center for Bio\/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA"}]},{"given":"Scott A.","family":"Trammell","sequence":"additional","affiliation":[{"name":"Center for Bio\/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA"}]},{"given":"Jeffrey R.","family":"Deschamps","sequence":"additional","affiliation":[{"name":"Center for Bio\/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA"}]},{"given":"Anne W.","family":"Kusterbeck","sequence":"additional","affiliation":[{"name":"Center for Bio\/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA"}]}],"member":"1968","published-online":{"date-parts":[[2010,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/0043-1354(76)90191-3","article-title":"The toxic effects of trinitrotoluene (TNT) and its primary degradation products on two species of algae and the fathead minnow","volume":"10","author":"Smock","year":"1976","journal-title":"Water Res"},{"key":"ref_2","unstructured":"USEPA (2002). 2,4,6-Trinitrotoluene in System, USEPA."},{"key":"ref_3","unstructured":"USEPA (2006). 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