{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:04:50Z","timestamp":1761393890026,"version":"build-2065373602"},"reference-count":22,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2010,3,2]],"date-time":"2010-03-02T00:00:00Z","timestamp":1267488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A new fluorescent sensor for nitric oxide (NO) is presented that is based on its reaction with a non fluorescent substance, reduced fluoresceinamine, producing the highly fluorescent fluoresceinamine. Using a portable homemade stabilized light source consisting of 450 nm LED and fiber optics to guide the light, the sensor responds linearly within seconds in the NO concentration range between about 10\u2013750 \u03bcM with a limit of detection (LOD) of about 1 \u03bcM. The system generated precise intensity readings, with a relative standard deviation of less than 1%. The suitability of the sensor was assessed by monitoring the NO generated by either the nitrous acid decomposition reaction or from a NO-releasing compound. Using relatively high incubation times, the sensor also responds quantitatively to hydrogen peroxide and potassium superoxide, however, using transient signal measurements results in no interfering species.<\/jats:p>","DOI":"10.3390\/s100301661","type":"journal-article","created":{"date-parts":[[2010,3,2]],"date-time":"2010-03-02T11:15:22Z","timestamp":1267528522000},"page":"1661-1669","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Reduced Fluoresceinamine as a Fluorescent Sensor for Nitric Oxide"],"prefix":"10.3390","volume":"10","author":[{"given":"Abel  J.","family":"Duarte","sequence":"first","affiliation":[{"name":"REQUIMTE, Instituto Superior de Engenharia do Porto, R. Ant\u00f3nio Bernardino Almeida 431, 4200-072 Porto, Portugal"}]},{"given":"Joaquim C.G.","family":"Esteves da Silva","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Qu\u00edmica, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Rua Campo Alegre 687, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2010,3,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/sj.bjp.0705776","article-title":"Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?","volume":"142","author":"Halliwell","year":"2004","journal-title":"British J. 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