{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T12:48:28Z","timestamp":1760705308103,"version":"build-2065373602"},"reference-count":15,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2010,3,12]],"date-time":"2010-03-12T00:00:00Z","timestamp":1268352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-g (IFN-g) capture antibody was immobilized on the gold NIs, followed by binding of IFN-g to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.<\/jats:p>","DOI":"10.3390\/s100302045","type":"journal-article","created":{"date-parts":[[2010,3,14]],"date-time":"2010-03-14T13:26:04Z","timestamp":1268573164000},"page":"2045-2053","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR)"],"prefix":"10.3390","volume":"10","author":[{"given":"Tae-Han","family":"Lee","sequence":"first","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea"},{"name":"University of Science and Technology (UST), 305-333 Daejeon, Korea"}]},{"given":"Seung-Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea"}]},{"given":"Ji-Ae","family":"Jung","sequence":"additional","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea"},{"name":"University of Science and Technology (UST), 305-333 Daejeon, Korea"}]},{"given":"Junhyoung","family":"Ahn","sequence":"additional","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea"},{"name":"University of Science and Technology (UST), 305-333 Daejeon, Korea"}]},{"given":"Min-Gon","family":"Kim","sequence":"additional","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea"},{"name":"University of Science and Technology (UST), 305-333 Daejeon, Korea"}]},{"given":"Yong-Beom","family":"Shin","sequence":"additional","affiliation":[{"name":"Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea"},{"name":"University of Science and Technology (UST), 305-333 Daejeon, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2010,3,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1021\/ac015657x","article-title":"A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on surface","volume":"74","author":"Nath","year":"2002","journal-title":"Anal. 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