{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:56:45Z","timestamp":1792731405329,"version":"4.5.0"},"reference-count":66,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2010,5,4]],"date-time":"2010-05-04T00:00:00Z","timestamp":1272931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Aptamers have been widely used as recognition elements for biosensor construction, especially in the detection of proteins or small molecule targets, and regarded as promising alternatives for antibodies in bioassay areas. In this review, we present an overview of reported design strategies for the fabrication of biosensors and classify them into four basic modes: target-induced structure switching mode, sandwich or sandwich-like mode, target-induced dissociation\/displacement mode and competitive replacement mode. In view of the unprecedented advantages brought about by aptamers and smart design strategies, aptamer-based biosensors are expected to be one of the most promising devices in bioassay related applications.<\/jats:p>","DOI":"10.3390\/s100504541","type":"journal-article","created":{"date-parts":[[2010,5,4]],"date-time":"2010-05-04T11:18:03Z","timestamp":1272971883000},"page":"4541-4557","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":239,"title":["Design Strategies for Aptamer-Based Biosensors"],"prefix":"10.3390","volume":"10","author":[{"given":"Kun","family":"Han","sequence":"first","affiliation":[{"name":"Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China"},{"name":"Suzhou Institute of Biomedical Engineering Technology, Chinese Academy of Science, Suzhou 215163, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Liang","sequence":"additional","affiliation":[{"name":"Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China"},{"name":"School of Biotechnology and the Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2010,5,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","article-title":"In vitro selection of RNA molecules that bind specific ligands","volume":"346","author":"Ellington","year":"1990","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/355564a0","article-title":"Selection of single-stranded DNA molecules that bind and inhibit human thrombin","volume":"355","author":"Bock","year":"1992","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/0022-2836(91)90509-5","article-title":"Selexion: Systematic evolution of ligands by exponential enrichment with integrated optimization by non-linear analysis","volume":"222","author":"Irvine","year":"1991","journal-title":"J. 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