{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T06:18:05Z","timestamp":1778739485628,"version":"3.51.4"},"reference-count":60,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2010,5,27]],"date-time":"2010-05-27T00:00:00Z","timestamp":1274918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Routine determination of trace metals in complex media is still a difficult task for many analytical instruments. The aim of this work was to compare three electro-chemical instruments [a standard potentiostat (Autolab), a commercially available miniaturized potentiostat (PalmSens) and a homemade micropotentiostat] for easy-to-use and sensitive determination of cadmium(II) and lead(II) ions. The lowest detection limits (hundreds of pM) for both metals was achieved by using of the standard potentiostat, followed by the miniaturized potentiostat (tens of nM) and the homemade instrument (hundreds of nM). Nevertheless, all potentiostats were sensitive enough to evaluate contamination of the environment, because the environmental limits for both metals are higher than detection limits of the instruments. Further, we tested all used potentiostats and working electrodes on analysis of environmental samples (rainwater, flour and plant extract) with artificially added cadmium(II) and lead(II). Based on the similar results obtained for all potentiostats we choose a homemade instrument with a carbon tip working electrode for our subsequent environmental experiments, in which we analyzed maize and sunflower seedlings and rainwater obtained from various sites in the Czech Republic.<\/jats:p>","DOI":"10.3390\/s100605308","type":"journal-article","created":{"date-parts":[[2010,5,27]],"date-time":"2010-05-27T10:54:14Z","timestamp":1274957654000},"page":"5308-5328","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Electrochemical Microsensors for the Detection of Cadmium(II) and Lead(II) Ions in Plants"],"prefix":"10.3390","volume":"10","author":[{"given":"Olga","family":"Krystofova","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Libuse","family":"Trnkova","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic"},{"name":"Research Centre for Environmental Chemistry and Ecotoxicology, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vojtech","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Zehnalek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7496-2558","authenticated-orcid":false,"given":"Jaromir","family":"Hubalek","sequence":"additional","affiliation":[{"name":"Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Babula","sequence":"additional","affiliation":[{"name":"Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, CZ-612 42 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rene","family":"Kizek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2010,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0098-8472(98)00058-6","article-title":"Response to cadmium in higher plants","volume":"41","author":"Gabbrielli","year":"1999","journal-title":"Environ. Exp. Bot"},{"key":"ref_2","first-page":"569","article-title":"Image analysis and activity of intracellular esterases as new analytical tools for determination of growth and viability of embryonic cultures of spruce (Picea sp.) treated with cadmium","volume":"101","author":"Petrek","year":"2007","journal-title":"Chem. Listy"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"932","DOI":"10.3390\/s7060932","article-title":"Electroanalysis of plant thiols","volume":"7","author":"Supalkova","year":"2007","journal-title":"Sensors"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"743","DOI":"10.3390\/s7050743","article-title":"Multi-instrumental investigation of affecting of early somatic embryos of spruce by cadmium(II) and lead(II) ions","volume":"7","author":"Supalkova","year":"2007","journal-title":"Sensors"},{"key":"ref_5","first-page":"222","article-title":"Influence of heavy metals on production of protecting compounds in agriculture plants","volume":"120","author":"Zehnalek","year":"2004","journal-title":"Lis. Cukrov. Reparske"},{"key":"ref_6","first-page":"220","article-title":"Application of higher plants in phytoremetiation of heavy metals","volume":"120","author":"Zehnalek","year":"2004","journal-title":"Lis. Cukrov. Reparske"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1081\/AL-120029751","article-title":"Preconcentration-separation of heavy metal ions in environmental samples by membrane filtration-atomic absorption spectrometry combination","volume":"37","author":"Soylak","year":"2004","journal-title":"Anal. Lett"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1080\/00032710802013991","article-title":"ICP-OES and ICP-MS for the determination of metals: Application to oysters","volume":"41","author":"Sneddon","year":"2008","journal-title":"Anal. Lett"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.jhazmat.2007.03.077","article-title":"Review of procedures involving separation and preconcentration for the determination of cadmium using spectrometric techniques","volume":"145","author":"Ferreira","year":"2007","journal-title":"J. Hazard. Mater"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1080\/10408340600976473","article-title":"Online sample pretreatment systems for determination of cadmium by the ETAAS method","volume":"37","author":"Pyrzynska","year":"2007","journal-title":"Crit. Rev. Anal. Chem"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1080\/05704920500385486","article-title":"Determination of cadmium in biological samples","volume":"41","author":"Davis","year":"2006","journal-title":"Appl. Spectrosc. Rev"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1289\/ehp.10190","article-title":"Electrochemical sensors for the detection of lead and other toxic heavy metals: The next generation of personal exposure biomonitors","volume":"115","author":"Yantasee","year":"2007","journal-title":"Environ. Health Perspect"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.talanta.2007.03.036","article-title":"Atomic spectrometric methods for the determination of metals and metalloids in automotive fuels\u2014A review","volume":"73","author":"Korn","year":"2007","journal-title":"Talanta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.talanta.2005.10.043","article-title":"Separation and preconcentration procedures for the determination of lead using spectrometric techniques: A review","volume":"69","author":"Korn","year":"2006","journal-title":"Talanta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.envint.2003.09.009","article-title":"The determination of trace metal pollutants in enviromental matrices using ion chromatography","volume":"30","author":"Shaw","year":"2004","journal-title":"Environ. Int"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"582","DOI":"10.3390\/s8010582","article-title":"Using monoclonal antibody to determine lead ions with a localized surface plasmon resonance fiber-optic biosensor","volume":"8","author":"Lin","year":"2008","journal-title":"Sensors"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/j.jpba.2004.02.023","article-title":"A rapid ICP-MS screen for heavy metals in pharmaceutical compounds","volume":"35","author":"Lewen","year":"2004","journal-title":"J. Pharm. Biomed. Anal"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1021\/jf040032w","article-title":"Determination of cadmium, lead, and copper in margarines and butters by galvanostatic stripping chronopotentiometry","volume":"52","author":"Szlyk","year":"2004","journal-title":"J. Agric. Food Chem"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1002\/elan.200390085","article-title":"Amalgam electrodes for electroanalysis","volume":"15","author":"Mikkelsen","year":"2003","journal-title":"Electroanalysis"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0003-2670(03)00943-7","article-title":"Novel determination of cadmium ions using an enzyme self-assembled monolayer with surface plasmon resonance","volume":"500","author":"May","year":"2003","journal-title":"Anal. Chim. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1021\/ac990628w","article-title":"Simultaneous determination and speciation of zinc, cadmium, lead, and copper in natural water with minimum handling and artifacts, by voltammetry on a gel-integrated microelectrode array","volume":"72","author":"Pei","year":"2000","journal-title":"Anal. Chem"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1002\/(SICI)1521-4109(199808)10:10<701::AID-ELAN701>3.0.CO;2-I","article-title":"Anodic stripping of heavy metals using a hanging mercury drop electrode in a flow system","volume":"10","year":"1998","journal-title":"Electroanalysis"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1002\/elan.200703953","article-title":"Palladium biosensor","volume":"19","author":"Adam","year":"2007","journal-title":"Electroanalysis"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1002\/elan.200403264","article-title":"Study of metallothionein modified electrode surface behavior in the presence of heavy metal ions-biosensor","volume":"17","author":"Adam","year":"2005","journal-title":"Electroanalysis"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/elan.200603737","article-title":"A suggestion of electrochemical biosensor for study of platinum(II)-DNA interactions","volume":"19","author":"Krizkova","year":"2007","journal-title":"Electroanalysis"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5169","DOI":"10.1016\/j.electacta.2006.03.077","article-title":"Cisplatin electrochemical biosensor","volume":"51","author":"Petrlova","year":"2006","journal-title":"Electrochim. Acta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0003-2670(03)00718-9","article-title":"Mercury-free simultaneous determination of cadmium and lead at a glassy carbon electrode modified with multi-wall carbon nanotubes","volume":"489","author":"Wu","year":"2003","journal-title":"Anal. Chim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.aca.2003.10.001","article-title":"Simultaneous detection of cadmium, copper, and lead using a carbon paste electrode modified with carbamoylphosphonic acid self-assembled monolayer on mesoporous silica (SAMMS)","volume":"502","author":"Yantasee","year":"2004","journal-title":"Anal. Chim. Acta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0039-9140(03)00116-4","article-title":"Simultaneous determination of lead(II) and cadmium(II) at a diacetyldioxime modified carbon paste electrode by differential pulse stripping voltammetry","volume":"60","author":"Hu","year":"2003","journal-title":"Talanta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s006040050010","article-title":"Characterisation of screen-printed electrodes for detection of heavy metals","volume":"131","author":"Palchetti","year":"1999","journal-title":"Mikrochim. Acta"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.aca.2008.08.017","article-title":"Sensitive and stable monitoring of lead and cadmium in seawater using screen-printed electrode and electrochemical stripping analysis","volume":"627","author":"Guell","year":"2008","journal-title":"Anal. Chim. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s00216-003-2126-4","article-title":"Determination of lead and cadmium using a polycyclodextrin-modified carbon paste electrode with anodic stripping voltammetry","volume":"377","author":"Roa","year":"2003","journal-title":"Anal. Bioanal. Chem"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s11270-006-9223-x","article-title":"Electrochemical method for the rapid on site screening of cadmium and lead in soil and water samples","volume":"179","author":"Cooper","year":"2007","journal-title":"Water Air Soil Pollut"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.aca.2004.08.065","article-title":"Miniaturised stripping-based carbon modified sensor for in field analysis of heavy metals","volume":"530","author":"Palchetti","year":"2005","journal-title":"Anal. Chim. Acta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1002\/elan.200804192","article-title":"Peak area: The instrumental datum to improve the determination at ultratrace level concentration of mercury(II) at gold electrode","volume":"20","author":"Locatelli","year":"2008","journal-title":"Electroanalysis"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1002\/elan.200704026","article-title":"Voltammetric analysis of trace levels of platinum group metals principles and applications","volume":"19","author":"Locatelli","year":"2007","journal-title":"Electroanalysis"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1002\/elan.200603745","article-title":"Voltammetric peak area as instrumental datum. A possibility to improve the determination at ultratrace level concentration of platinum group metals (PGMs) and lead\u2014 Application to particulate matter","volume":"19","author":"Locatelli","year":"2007","journal-title":"Electroanalysis"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.electacta.2006.05.041","article-title":"Possible interference in the sequential voltammetric determination at trace and ultratrace concentration level of platinum group metals (PGMs) and lead\u2014Application to environmental matrices","volume":"52","author":"Locatelli","year":"2006","journal-title":"Electrochim. Acta"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.aca.2005.10.004","article-title":"Simultaneous square wave, stripping voltammetric determination of platinum group metals (PGMs) and lead at trace and ultratrace concentration level\u2014Application to surface water","volume":"557","author":"Locatelli","year":"2006","journal-title":"Anal. Chim. Acta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1007\/s00216-005-3356-4","article-title":"Determination of platinum-group metals and lead in vegetable environmental bio-monitors by voltammetric and spectroscopic techniques: critical comparison","volume":"382","author":"Locatelli","year":"2005","journal-title":"Anal. Bioanal. Chem"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1007\/s00216-004-3008-0","article-title":"Overlapping voltammetric peaks\u2014An analytical procedure for simultaneous determination of trace metals. Application to food and environmental matrices","volume":"381","author":"Locatelli","year":"2005","journal-title":"Anal. Bioanal. Chem"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/elan.1140050502","article-title":"Applications of Adsorptive Stripping Voltammetry in the Determination of Trace and Ultratrace Metals","volume":"5","author":"Paneli","year":"1993","journal-title":"Electroanalysis"},{"key":"ref_43","unstructured":"Wang, J. (1994). Analytical Electrochemistry, VCH Publishers."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1088\/0957-0233\/8\/1\/002","article-title":"Thick-film sensors: Past, present and future","volume":"8","author":"White","year":"1997","journal-title":"Meas. Sci. Technol"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1108\/13565361011009450","article-title":"New CMOS potentiostat as ASIC for several electrochemical microsensors construction","volume":"27","author":"Fujcik","year":"2009","journal-title":"Microelectron. Int"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"464","DOI":"10.3390\/s8010464","article-title":"Lactoferrin isolation using monolithic column coupled with spectrometric or micro-amperometric detector","volume":"8","author":"Adam","year":"2008","journal-title":"Sensors"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.3390\/s90705040","article-title":"Sunflower plants as bioindicators of environmental pollution with lead(II) ions","volume":"9","author":"Krystofova","year":"2009","journal-title":"Sensors"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.bioelechem.2003.08.007","article-title":"Electrochemical determination of lead and glutathione in a plant cell culture","volume":"63","author":"Vacek","year":"2004","journal-title":"Bioelectrochemistry"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.aca.2006.07.032","article-title":"Separation and determination of trace amounts of zinc, lead, cadmium and mercury in tap and Qaroun lake water using polyurethane foam functionalized with 4-hydroxytoluene and 4-hydroxyacetophenone","volume":"579","author":"Burham","year":"2006","journal-title":"Anal. Chim. Acta"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.snb.2007.07.025","article-title":"Utilizing a chronopotentiometric sensor technique for metallothionein determination in fish tissues and their host parasites","volume":"127","author":"Petrlova","year":"2007","journal-title":"Sens. Actuator B-Chem"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.3390\/s7071238","article-title":"Spectrometric and voltammetric analysis of urease-Nickel nanoelectrode as an electrochemical sensor","volume":"7","author":"Hubalek","year":"2007","journal-title":"Sensors"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.3390\/s6111483","article-title":"Electrochemical sensors for detection of acetylsalicylic acid","volume":"6","author":"Supalkova","year":"2006","journal-title":"Sensors"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"37","DOI":"10.17221\/2345-CJAS","article-title":"A sensor for investigating the interaction between biologically important heavy metals and glutathione","volume":"52","author":"Huska","year":"2007","journal-title":"Czech J. Anim. Sci"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5087","DOI":"10.1016\/j.electacta.2006.03.079","article-title":"Electrochemical study of S-nitrosoglutathione and nitric oxide by carbon fibre NO sensor and cyclic voltammetry\u2014possible way of monitoring of nitric oxide","volume":"51","author":"Vitecek","year":"2006","journal-title":"Electrochim. Acta"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s10534-004-5787-3","article-title":"Biosensors for heavy metals","volume":"18","author":"Verma","year":"2005","journal-title":"Biometals"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s00604-007-0900-2","article-title":"Detection of ultra-low activities of heavy metal ions by an array of potentiometric chemical sensors","volume":"163","author":"Rudnitskaya","year":"2008","journal-title":"Microchim. Acta"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.talanta.2008.02.039","article-title":"Determination of trace metals by anodic stripping voltammetry using a bismuth-modified carbon nanotube electrode","volume":"76","author":"Hwang","year":"2008","journal-title":"Talanta"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1002\/elan.200704088","article-title":"Anodic stripping voltammetric determination of lead in tap water at an ordered mesoporous carbon\/Nafion composite film electrode","volume":"20","author":"Zhu","year":"2008","journal-title":"Electroanalysis"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1002\/elan.200603850","article-title":"A potentiometric stripping analyzer for multianalyte screening","volume":"19","author":"Adami","year":"2007","journal-title":"Electroanalysis"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.snb.2006.11.033","article-title":"An electrochemical sensor for Cd2+ based on the inducing adsorption ability of I","volume":"124","author":"Li","year":"2007","journal-title":"Sens. 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