{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:38:36Z","timestamp":1760243916392,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2010,6,8]],"date-time":"2010-06-08T00:00:00Z","timestamp":1275955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag\/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 \u00b5M in phosphate buffer and bovine serum media.<\/jats:p>","DOI":"10.3390\/s100605758","type":"journal-article","created":{"date-parts":[[2010,6,8]],"date-time":"2010-06-08T12:00:40Z","timestamp":1275998440000},"page":"5758-5773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor"],"prefix":"10.3390","volume":"10","author":[{"given":"Shu-Yi","family":"Hsu","sequence":"first","affiliation":[{"name":"Department of Materials Science and Engineering, National Chung Hsing University, Taichung 40227, Taiwan"}]},{"given":"Brandon","family":"Bartling","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA"}]},{"given":"Christina","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences, Cornell University, 147 Goldwin Smith Hall, Ithaca, NY 14853-3201, USA"}]},{"given":"Fuh-Sheng","family":"Shieu","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering, National Chung Hsing University, Taichung 40227, Taiwan"}]},{"given":"Chung-Chiun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA"}]}],"member":"1968","published-online":{"date-parts":[[2010,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1126\/science.1411571","article-title":"Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C","volume":"258","author":"Nishizuka","year":"1992","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1096\/fasebj.9.7.7737456","article-title":"Protein kinase C and lipid signaling for sustained cellular responses","volume":"9","author":"Nishizuka","year":"1995","journal-title":"FASEB J"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.1016\/S0021-9258(17)41834-5","article-title":"The sphingomyelin cycle and the second messenger function of ceramide","volume":"269","author":"Hannun","year":"1994","journal-title":"J Biol. 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