{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T05:13:40Z","timestamp":1769836420341,"version":"3.49.0"},"reference-count":33,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2010,6,9]],"date-time":"2010-06-09T00:00:00Z","timestamp":1276041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The ultimate goal in any biosensor development project is its use for actual sample detection. Recently, there has been an interest in biosensors with aptamers as bio-recognition elements, but reported examples all deal with standards, not human serum. In order to verify the differences of aptamer-based biosensor and antibody-based biosensor in clinical detection, a comparison of the performance of aptamer-based and antibody-based quartz crystal microbalance (QCM) biosensors for the detection of immunoglobulin E (IgE) in human serum was carried out. Aptamers (or antibodies) specific to IgE were immobilized on the gold surface of a quartz crystal. The frequency shifts of the QCM were measured. The linear range with the antibody (10\u2013240 \u03bcg\/L) compared to that of the aptamer (2.5\u2013200 \u03bcg\/L), but a lower detection limit could be observed in the aptamer-based biosensor. The reproducibility of the two biosensors was comparable. The aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity. In addition, the aptamer receptors could tolerate repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. When stored for three weeks, the frequency shifts of the aptamer-coated crystals were all greater than 90% of those on the response at the first day.<\/jats:p>","DOI":"10.3390\/s100605859","type":"journal-article","created":{"date-parts":[[2010,6,9]],"date-time":"2010-06-09T11:22:42Z","timestamp":1276082562000},"page":"5859-5871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Development of a Quartz Crystal Microbalance Biosensor with Aptamers as Bio-recognition Element"],"prefix":"10.3390","volume":"10","author":[{"given":"Chunyan","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China"}]},{"given":"Tangyou","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Daping Hospital, Third Military Medical University, Chongqing 400042, China"}]},{"given":"Yongzhi","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the General Navy Hospital, Beijing 100037, China"}]},{"given":"Yuhui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China"}]},{"given":"Han","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China"}]},{"given":"Weiling","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China"}]}],"member":"1968","published-online":{"date-parts":[[2010,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","article-title":"In vitro selection of RNA molecules that bind specific ligands","volume":"346","author":"Ellington","year":"1990","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","article-title":"Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1006\/abio.1996.0462","article-title":"Detecting immobilized protein kinase C isozymes with RNA aptamers","volume":"242","author":"Conrad","year":"1996","journal-title":"Anal. Biochem"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"11416","DOI":"10.1074\/jbc.M109752200","article-title":"A Y2 receptor mimetic aptamer directed against neuropeptide Y","volume":"277","author":"Proske","year":"2002","journal-title":"J. Biol. Chem"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0378-4347(99)00243-1","article-title":"Aptamer affinity chromatography: combinatorial chemistry applied to protein purification","volume":"731","author":"Romig","year":"1999","journal-title":"J. Chrom. B Biomed. Sci. Appl"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.cbpa.2005.06.011","article-title":"Aptamers as tools for target validation","volume":"9","author":"Blank","year":"2005","journal-title":"Curr. Opin. Chem. Biol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.drudis.2006.02.007","article-title":"Peptide aptamers as guides for small-molecule drug discovery","volume":"11","author":"Baines","year":"2006","journal-title":"Drug Discov. Today"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.ab.2009.01.046","article-title":"An aptamer-based biosensor for mammalian initiation factor eukaryotic initiation factor 4A","volume":"388","author":"Oguro","year":"2009","journal-title":"Anal. Biochem"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1146\/annurev.med.56.062904.144915","article-title":"Aptamers: an emerging class of therapeutics","volume":"56","author":"Nimjee","year":"2005","journal-title":"Annu. Rev. Med"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ab.2007.09.007","article-title":"DNA aptamer with high affinity and specificity for therapeutic anthracyclines","volume":"373","author":"Wochner","year":"2008","journal-title":"Anal. Biochem"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1016\/j.bios.2004.11.006","article-title":"Analytical applications of aptamers","volume":"20","author":"Tombelli","year":"2005","journal-title":"Biosens. Bioelectron"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.bios.2009.06.001","article-title":"Label-free and sensitive faradic impedance aptasensor for the determination of lysozyme based on target-induced aptamer displacement","volume":"25","author":"Peng","year":"2009","journal-title":"Biosens. Bioelectron"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3915","DOI":"10.1093\/nar\/26.17.3915","article-title":"Staining of cell surface human CD4 with 2\u2032-F-pyrimidine-containing RNA aptamers for flow cytometry","volume":"26","author":"Davis","year":"1998","journal-title":"Nucleic. Acids. Res"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.aca.2008.12.025","article-title":"Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip","volume":"634","author":"Kim","year":"2009","journal-title":"Anal. Chim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s00253-005-0193-5","article-title":"Aptamers\u2014Basic research, drug development, and clinical applications","volume":"69","author":"Proske","year":"2005","journal-title":"Appl. Microbiol. Biotechnol"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.aca.2009.02.018","article-title":"Application and analysis of structure-switching aptamers for small molecule quantification","volume":"638","author":"Xie","year":"2009","journal-title":"Anal. Chim. Acta"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.aca.2008.08.041","article-title":"A electrochemiluminescence aptasensor for detection of thrombin incorporating the capture aptamer labeled with gold nanoparticles immobilized onto the thio-silanized ITO electrode","volume":"628","author":"Fang","year":"2008","journal-title":"Anal. Chim. Acta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7668","DOI":"10.1158\/0008-5472.CAN-04-2550","article-title":"Nanoparticle-aptamer bioconjugates: a new approach for targeting prostate cancer cells","volume":"64","author":"Farokhzad","year":"2004","journal-title":"Cancer Res"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1021\/ac061879p","article-title":"Aptamer-based detection of plasma proteins by an electrochemical assay coupled to magnetic beads","volume":"79","author":"Centi","year":"2007","journal-title":"Anal. Chem"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4488","DOI":"10.1021\/ac011294p","article-title":"An aptamer-based quartz crystal protein biosensor","volume":"74","author":"Liss","year":"2002","journal-title":"Anal. Chem"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/j.bios.2004.03.037","article-title":"Development of biosensors with aptamers as bio-recognition element: the case of HIV-1 Tat protein","volume":"20","author":"Minunni","year":"2004","journal-title":"Biosens. Bioelectron"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/S0956-5663(99)00040-8","article-title":"Commercial quartz crystal microbalances\u2014Theory and applications","volume":"14","author":"Guilbault","year":"1999","journal-title":"Biosens. Bioelectron"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0956-5663(03)00260-4","article-title":"A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin","volume":"19","author":"Zhang","year":"2004","journal-title":"Biosens. Bioelectron"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1373\/clinchem.2006.073569","article-title":"Rapid and simultaneous quantification of 4 urinary proteins by piezoelectric quartz crystal microbalance immunosensor array","volume":"52","author":"Luo","year":"2006","journal-title":"Clin. Chem"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1016\/j.bios.2008.12.036","article-title":"Aptamer-based piezoelectric quartz crystal microbalance biosensor array for the quantification of IgE","volume":"24","author":"Yao","year":"2009","journal-title":"Biosens. Bioelectron"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4540","DOI":"10.1021\/ac980638h","article-title":"Aptamera as ligands in affinity probe capillary electrophoresis","volume":"70","author":"German","year":"1998","journal-title":"Anal. Chem"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/01971529108055060","article-title":"Rapid detection of antigen-specific mouse IgE by enzyme-linked immunosorbent assay (ELISA)","volume":"12","author":"Strouse","year":"1991","journal-title":"J. Immunoassay"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5230","DOI":"10.1021\/ac049565u","article-title":"Signaling aptamer\/protein binding by a molecular light switch complex","volume":"76","author":"Jiang","year":"2004","journal-title":"Anal. Chem"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1021\/ac0483926","article-title":"DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy","volume":"77","author":"Gokulrangan","year":"2005","journal-title":"Anal. Chem"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.snb.2006.09.024","article-title":"Viability of allergy (IgE) detection using an alternative aptamer receptor and electrochemical means","volume":"121","author":"Papamichael","year":"2007","journal-title":"Sens. Actuat. B"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"221","DOI":"10.4049\/jimmunol.157.1.221","article-title":"High-affinity oligonucleotide ligands to human IgE inhibit binding to Fc epsilon receptor I","volume":"157","author":"Wiegand","year":"1996","journal-title":"J. Immunol"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.aca.2006.01.016","article-title":"Comparison of antibody and aptamer receptors for the specific detection of thrombin with a nanometer gap-sized impedance biosensor","volume":"573\u2013574","author":"Schlecht","year":"2006","journal-title":"Anal. Chim. Acta"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/S0956-5663(02)00084-2","article-title":"Improved procedures for immobilization of oligonucleotides on gold coated piezoelectric quartz crystals","volume":"17","author":"Tombelli","year":"2002","journal-title":"Biosens. 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