{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:38:46Z","timestamp":1767706726258,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2010,6,21]],"date-time":"2010-06-21T00:00:00Z","timestamp":1277078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Retinal stem cells bear potency of proliferation, self-renewal, and differentiation into many retinal cells. Utilizing appropriate sensors one can effectively detect the self-renewal and aging process abilities. Silencing information regulator (SirT1), a member of the sirtuin family, is a NAD-dependent histone deacetylase and an essential mediator for longevity in normal cells by calorie restriction. We firstly investigate the SirT1 mRNA expression in retinal stem cells from rats and 19 human eyes of different ages. Results revealed that SirT1 expression was significantly decreased in in vivo aged eyes, associated with poor self-renewal abilities. Additionally, SirT1 mRNA levels were dose-dependently increased in resveratrol- treated retinal stem cells. The expression of SirT1 on oxidative stress-induced damage was significantly decreased, negatively correlated with the level of intracellular reactive oxygen species production. Treatment with resveratrol could effectively further reduce oxidative stress induced by H2O2 treatment in retinal stem cells. Importantly, the anti-oxidant effects of resveratrol in H2O2-treated retinal stem cells were significantly abolished by knockdown of SirT1 expression (sh-SirT1). SirT1 expression provides a feasible sensor in assessing self-renewal and aging process in retinal stem cells. Resveratrol can prevent reactive oxygen species-induced damages via increased retinal SirT1 expression.<\/jats:p>","DOI":"10.3390\/s100606172","type":"journal-article","created":{"date-parts":[[2010,6,22]],"date-time":"2010-06-22T02:13:46Z","timestamp":1277172826000},"page":"6172-6194","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["SirT1\u2014A Sensor for Monitoring Self-Renewal and Aging Process in Retinal Stem Cells"],"prefix":"10.3390","volume":"10","author":[{"given":"Chi-Hsien","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Shin Kong Wu Ho-Su Memorial Hospital and Fu-Jen Catholic University, Taipei 11101, Taiwan"},{"name":"Department of Ophthalmology, Taipei Veterans General Hospital, 201Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"}]},{"given":"Yuh-Lih","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, 201 Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Institute of Pharmacology, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"}]},{"given":"Chung-Lan","family":"Kao","sequence":"additional","affiliation":[{"name":"Department of Physical Medicine & Rehabilitation and Surgery, Taipei Veterans General Hospital, 201Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"}]},{"given":"Ling-Min","family":"Tseng","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, 201 Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Department of Physical Medicine & Rehabilitation and Surgery, Taipei Veterans General Hospital, 201Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"}]},{"given":"Chih-Chia","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Taipei Veterans General Hospital, 201Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"}]},{"given":"Yu-Chih","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, 201 Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"}]},{"given":"Ching-Yao","family":"Tsai","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"},{"name":"Department of Ophthalmology, Taipei City Hospital, Taipei, Taiwan"}]},{"given":"Lin-Chung","family":"Woung","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"},{"name":"Department of Ophthalmology, Taipei City Hospital, Taipei, Taiwan"}]},{"given":"Jorn-Hon","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Cheng Hsin General Hospital, Taiwan"}]},{"given":"Shih-Hwa","family":"Chiou","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education, Taipei Veterans General Hospital, 201 Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Institute of Pharmacology, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"},{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"}]},{"given":"Shih-Jen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Taipei Veterans General Hospital, 201Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Department of Physical Medicine & Rehabilitation and Surgery, Taipei Veterans General Hospital, 201Shih-Pai, Road, Section 2, Taipei 11217, Taiwan"},{"name":"Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei 11221, Taiwan"}]}],"member":"1968","published-online":{"date-parts":[[2010,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2570","DOI":"10.1101\/gad.13.19.2570","article-title":"The SIR2\/3\/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms","volume":"13","author":"Kaeberlein","year":"1999","journal-title":"Genes 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