{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T15:17:30Z","timestamp":1766157450625,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A surface plasmon resonance (SPR) immunosensor was developed for the detection of E. coli O157:H7 by means of a new subtractive inhibition assay. In the subtractive inhibition assay, E. coli O157:H7 cells and goat polyclonal antibodies for E. coli O157:H7 were incubated for a short of time, and then the E. coli O157:H7 cells which bound antibodies were removed by a stepwise centrifugation process. The remaining free unbound antibodies were detected through interaction with rabbit anti-goat IgG polyclonal antibodies immobilized on the sensor chip using a BIAcore 3000 biosensor. The results showed that the signal was inversely correlated with the concentration of E. coli O157:H7 cells in a range from 3.0 \u00d7 104 to 3.0 \u00d7 108 cfu\/mL with a detection limit of 3.0 \u00d7 104 cfu\/mL. Compared with direct SPR by immobilizing antibodies on the chip surface to capture the bacterial cells and ELISA for E. coli O157:H7 (detection limit: both 3.0 \u00d7 105 cfu\/mL in this paper), the detection limit of subtractive inhibition assay method was reduced by one order of magnitude. The method simplifies bacterial cell detection to protein-protein interaction, which has the potential for providing a practical alternative for the monitoring of E. coli O157:H7 and other pathogens.<\/jats:p>","DOI":"10.3390\/s110302728","type":"journal-article","created":{"date-parts":[[2011,3,2]],"date-time":"2011-03-02T19:57:04Z","timestamp":1299095824000},"page":"2728-2739","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Subtractive Inhibition Assay for the Detection of E. coli O157:H7 Using Surface Plasmon Resonance"],"prefix":"10.3390","volume":"11","author":[{"given":"Yixian","family":"Wang","sequence":"first","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, Zhejiang, China"}]},{"given":"Zunzhong","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, Zhejiang, China"}]},{"given":"Chengyan","family":"Si","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, Zhejiang, China"}]},{"given":"Yibin","family":"Ying","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, Zhejiang, China"}]}],"member":"1968","published-online":{"date-parts":[[2011,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"607","DOI":"10.3201\/eid0505.990502","article-title":"Food-related illness and death in the United States","volume":"5","author":"Mead","year":"1999","journal-title":"Emerg. 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