{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:20:07Z","timestamp":1764688807643,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2011,3,24]],"date-time":"2011-03-24T00:00:00Z","timestamp":1300924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0:17 mM) glucose determination with a detection range from 0:4 mM to 5 mM.<\/jats:p>","DOI":"10.3390\/s110403483","type":"journal-article","created":{"date-parts":[[2011,3,25]],"date-time":"2011-03-25T11:14:25Z","timestamp":1301051665000},"page":"3483-3497","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase"],"prefix":"10.3390","volume":"11","author":[{"given":"Rosario","family":"Esposito","sequence":"first","affiliation":[{"name":"CNISM-Dipartimento Scienze Fisiche, Universit\u00e0 di Napoli Federico II, Napoli 80126, Italy"}]},{"given":"Bartolomeo","family":"Della Ventura","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Seconda Universit\u00e0 di Napoli, Napoli 80138, Italy"}]},{"given":"Sergio","family":"De Nicola","sequence":"additional","affiliation":[{"name":"Istituto Nazionale di Ottica, CNR, compr. 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Olivetti, Via Campi Flegrei 34, Pozzuoli 80078, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2270-9128","authenticated-orcid":false,"given":"Carlo","family":"Altucci","sequence":"additional","affiliation":[{"name":"CNISM-Dipartimento Scienze Fisiche, Universit\u00e0 di Napoli Federico II, Napoli 80126, Italy"}]},{"given":"Raffaele","family":"Velotta","sequence":"additional","affiliation":[{"name":"CNISM-Dipartimento Scienze Fisiche, Universit\u00e0 di Napoli Federico II, Napoli 80126, Italy"}]},{"given":"Damiano Gustavo","family":"Mita","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Seconda Universit\u00e0 di Napoli, Napoli 80138, Italy"},{"name":"Institute of Genetics and Biophysics, CNR, Naples 80131, Italy"}]},{"given":"Maria","family":"Lepore","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Seconda Universit\u00e0 di Napoli, Napoli 80138, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2011,3,24]]},"reference":[{"key":"ref_1","unstructured":"Wolfbeis, OS (2008). 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