{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T06:59:02Z","timestamp":1763017142907,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2011,8,15]],"date-time":"2011-08-15T00:00:00Z","timestamp":1313366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.<\/jats:p>","DOI":"10.3390\/s110808018","type":"journal-article","created":{"date-parts":[[2011,8,15]],"date-time":"2011-08-15T10:36:49Z","timestamp":1313404609000},"page":"8018-8027","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface"],"prefix":"10.3390","volume":"11","author":[{"given":"Gang","family":"Liu","sequence":"first","affiliation":[{"name":"Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"},{"name":"Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China"}]},{"given":"Ruojun","family":"Lao","sequence":"additional","affiliation":[{"name":"Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}]},{"given":"Qin","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}]},{"given":"Lanying","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China"}]},{"given":"Hao","family":"Shen","sequence":"additional","affiliation":[{"name":"Institute of Inorganic Chemistry, University of Cologne, Cologne 50939, Germany"}]},{"given":"Sanjay","family":"Mathur","sequence":"additional","affiliation":[{"name":"Institute of Inorganic Chemistry, University of Cologne, Cologne 50939, Germany"}]},{"given":"Chunhai","family":"Fan","sequence":"additional","affiliation":[{"name":"Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China"}]},{"given":"Shiping","family":"Song","sequence":"additional","affiliation":[{"name":"Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China"}]}],"member":"1968","published-online":{"date-parts":[[2011,8,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1186\/1297-9686-34-3-275","article-title":"A review on SNP and other types of molecular markers and their use in animal genetics","volume":"34","author":"Vignal","year":"2002","journal-title":"Genet. 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