{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T08:01:02Z","timestamp":1761292862472,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2011,11,3]],"date-time":"2011-11-03T00:00:00Z","timestamp":1320278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs) fluorescent probe and its application for specific detection of recombinant human erythropoietin-\u03b1 (rHuEPO-\u03b1). In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor), on the surface of which the complementary sequences are linked (as cODN-AuNPs) and pre-hybridized with carboxymethylfluorescein (FAM)-labeled anti-rHuEPO-\u03b1 aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-\u03b1 concentrations as low as 0.92 nM within a few hours.<\/jats:p>","DOI":"10.3390\/s111110490","type":"journal-article","created":{"date-parts":[[2011,11,4]],"date-time":"2011-11-04T03:22:56Z","timestamp":1320376976000},"page":"10490-10501","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-\u03b1"],"prefix":"10.3390","volume":"11","author":[{"given":"Jiefang","family":"Sun","sequence":"first","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, No. 27, Taiping Road, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aitao","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pathology, General Hospital of the Chinese People\u2019s Liberation Army, No. 28, Fuxing Road, Beijing 100853, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, No. 27, Taiping Road, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4780-712X","authenticated-orcid":false,"given":"Lei","family":"Guo","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, No. 27, Taiping Road, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianwei","family":"Xie","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, No. 27, Taiping Road, Beijing 100850, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2011,11,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1126\/science.1080664","article-title":"\u201cPlugging into enzymes\u201d: Nanowiring of redox enzymes by a gold nanoparticle","volume":"21","author":"Xiao","year":"2003","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4700","DOI":"10.1021\/ja0296935","article-title":"Multifunctional gold nanoparticle-peptide complexes for nuclear targeting","volume":"125","author":"Tkachenko","year":"2003","journal-title":"J. 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