{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T03:53:16Z","timestamp":1769917996740,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2011,11,16]],"date-time":"2011-11-16T00:00:00Z","timestamp":1321401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A piezoelectric immunosensor based on gold nanoparticles (AuNPs) co-immobilized on a dithiol-modified surface is proposed for detection of human cardiac troponin T (TnT). Anti-human troponin T (anti-TnT) antibodies were covalently immobilized on the nanostructured electrode surface by thiol-aldehyde linkages. In a homogeneous bulk solution, TnT was captured by anti-TnT immobilized on the QCM electrode. Cyclic voltammetry studies were used to characterize the AuNPs layer on the electrode surface and the anti-TnT immobilization steps. The QCM-flow immunosensor exhibited good reliability, measuring concentrations of TnT from 0.003 to 0.5 ng mL\u22121 in human serum with high linearity (r = 0.989; p &lt; 0.01). The immunosensor exhibited a 7% coefficient of variation and 0.0015 ng mL\u22121 limit of detection, indicating a high reproducibility and sensitivity. The proposed QCM nanostructured immunosensor is easy to use and has promising potential in the diagnosis of acute myocardial infarction due to its speed and high sensitivity.<\/jats:p>","DOI":"10.3390\/s111110785","type":"journal-article","created":{"date-parts":[[2011,11,16]],"date-time":"2011-11-16T20:34:54Z","timestamp":1321475694000},"page":"10785-10797","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["A Nanostructured Piezoelectric Immunosensor for Detection of Human Cardiac Troponin T"],"prefix":"10.3390","volume":"11","author":[{"given":"Rosana A. S.","family":"Fonseca","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Engenharia Biom\u00e9dica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, Pernambuco 50670-901, Brazil"}]},{"given":"Joilson","family":"Ramos-Jesus","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Engenharia Biom\u00e9dica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, Pernambuco 50670-901, Brazil"}]},{"given":"Lauro T.","family":"Kubota","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade Estadual de Campinas, Campinas 04118-080, SP, Brazil"}]},{"given":"Rosa F.","family":"Dutra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Engenharia Biom\u00e9dica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235, Recife, Pernambuco 50670-901, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2011,11,16]]},"reference":[{"key":"ref_1","unstructured":"Allender, S., Scarborough, P., Peto, V., Rayner, M., Leal, J., Luengo-Fernandez, R., and Gray, A. (2008). European Cardiovascular Disease Statistics, European Heart Network."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S0735-1097(00)00804-4","article-title":"Myocardial infarction redefined: A consensus document of the joint European society of cardiology\/american college of cardiology committee for the redefinition of myocardial infarction","volume":"36","author":"Alpert","year":"2000","journal-title":"J. Am. Coll. Cardiol"},{"key":"ref_3","unstructured":"Anu\u00e1rio Estat\u00edstico de Sa\u00fade do Brasil 2001. Available online: http:\/\/portal.saude.gov.br\/saude\/aplicacoes\/anuario2001\/index.cfm\/ (accessed on 28 January 2010)."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1093\/clinchem\/45.7.1104","article-title":"National academy of clinical biochemistry standards of laboratory practice: Recommendations for the use of cardiac markers in coronary artery diseases","volume":"45","author":"Wu","year":"1999","journal-title":"Clin. Chem"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1023\/A:1005397501968","article-title":"Troponin T: Genetics, properties and function","volume":"19","author":"Perry","year":"1998","journal-title":"J. Muscle Res. Cell Motil"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S63","DOI":"10.1016\/S0049-3848(01)00299-7","article-title":"The diagnostic role of troponins","volume":"103","author":"Hamm","year":"2001","journal-title":"Thromb. Res"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.abb.2010.10.013","article-title":"Troponin T isoforms and posttranscriptional modifications: Evolution, regulation and function","volume":"505","author":"Wei","year":"2011","journal-title":"Arch. Biochem. Biophys"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.bbrc.2007.11.187","article-title":"Troponin: Regulatory function and disorders","volume":"369","author":"Ohtsuki","year":"2008","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8235","DOI":"10.1021\/ac101657h","article-title":"Development of a troponin I biosensor using a peptide obtained through phage display","volume":"82","author":"Wu","year":"2010","journal-title":"Anal. Chem"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.clinbiochem.2006.05.011","article-title":"Cardiac markers and their point-of-care testing for diagnosis of acute myocardial infarction","volume":"39","author":"Yang","year":"2006","journal-title":"Clin. Biochem"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1136\/heart.83.4.371","article-title":"Troponin T or troponin I as cardiac markers in ischaemic heart disease","volume":"83","author":"Maynard","year":"2000","journal-title":"Heart"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1007\/BF01337937","article-title":"Use of a quartz vibrator for weighing thin layers on a microbalance","volume":"155","author":"Sauerbrey","year":"1959","journal-title":"Z. Phys"},{"key":"ref_13","first-page":"121","article-title":"Electrochemical biosensors: Recommended definitions and classification","volume":"16","author":"Thevenot","year":"2001","journal-title":"Biosens. Bioelectron"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.cca.2006.07.029","article-title":"An SPR immunosensor for human cardiac troponin T using specific binding avidin to biotin at carboxymethyldextran-modified gold chip","volume":"376","author":"Dutra","year":"2007","journal-title":"Clin. Chim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.bios.2005.12.008","article-title":"A disposable electrochemical immunosensor for flow injection immunoassay of carcinoembryonic antigen","volume":"22","author":"Wu","year":"2006","journal-title":"Biosens. Bioelectron"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1016\/j.bios.2008.11.016","article-title":"A QCM-D-based biosensor for E. coli O157:H7 highlighting the relevance of the dissipation slope as a transduction signal","volume":"24","author":"Poitras","year":"2009","journal-title":"Biosens. Bioelectron"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S1389-0352(01)00058-7","article-title":"Amperometric enzyme biosensors based on optimized electron-transfer pathways and non-manual immobilisation procedures","volume":"82","author":"Schuhmann","year":"2002","journal-title":"Rev. Mol. Biotech"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.snb.2006.10.001","article-title":"Surface plasmon resonance based fiber-optic sensor for the detection of pesticide","volume":"123","author":"Chand","year":"2007","journal-title":"Sens. Actuat. B"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5053","DOI":"10.3390\/molecules15075053","article-title":"An amperometric immunosensor based on a polyelectrolyte\/gold magnetic nanoparticle supramolecular assembly\u2014Modified electrode for the determination of HIV p24 in serum","volume":"15","author":"Gan","year":"2010","journal-title":"Molecules"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"7335","DOI":"10.1021\/ac101319k","article-title":"Multifunctional Au nanoparticle dendrimer-based surface plasmon resonance biosensor and its application for improved insulin detection","volume":"82","author":"Frasconi","year":"2010","journal-title":"Anal. Chem"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/S0956-5663(99)00040-8","article-title":"Commercial quartz crystal microbalances\u2014Theory and Applications","volume":"14","author":"Guilbault","year":"1999","journal-title":"Biosens. Bioelectron"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1006\/abio.2001.5409","article-title":"Comparison of different protein immobilization methods on quartz crystal microbalance surface in flow injection immunoassay","volume":"299","author":"Liu","year":"2001","journal-title":"Anal. Biochem"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.snb.2005.06.014","article-title":"Quartz crystal microbalance immunoassay with dendritic amplification using colloidal gold immunocomplex","volume":"114","author":"Chu","year":"2006","journal-title":"Sens. Actuat. B"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jim.2007.12.019","article-title":"A reusable piezoelectric immunosensor using antibody-adsorbed magnetic nanocomposite","volume":"332","author":"Zhang","year":"2008","journal-title":"J. Immunol. Meth"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1002\/elan.200503415","article-title":"Application of nanoparticles in electrochemical sensors and biosensors","volume":"18","author":"Luo","year":"2006","journal-title":"Electroanalysis"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1016\/j.jpba.2006.12.013","article-title":"Surface plasmon resonance immunosensor for human cardiac troponin T based on self-assembled monolayer","volume":"43","author":"Dutra","year":"2007","journal-title":"J. Pharm. Biomed. Anal"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1016\/S0039-9140(97)00392-5","article-title":"Piezoelectric quartz crystal biosensors","volume":"46","author":"Bunde","year":"1998","journal-title":"Talanta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1016\/j.bios.2009.01.014","article-title":"Piezoelectric immunosensor with gold nanoparticles enhanced competitive immunoreaction technique for quantification of aflatoxin B1","volume":"24","author":"Jin","year":"2009","journal-title":"Biosens. Bioelectron"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.aca.2004.09.075","article-title":"A novel capacitive immunosensor based on gold colloid monolayers associated with a sol-gel matrix","volume":"528","author":"Wu","year":"2005","journal-title":"Anal. Chim. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2454","DOI":"10.1016\/j.bios.2004.11.003","article-title":"Nanoparticle labels in immunosensing using optical detection methods","volume":"20","author":"Seydack","year":"2005","journal-title":"Biosens. Bioelectron"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jelechem.2007.02.027","article-title":"Amplification of antigen-antibody interactions via back-filling of HRP (Horseradish peroxidase) on the layer-by-layer self-assembling of thionine and gold nanoparticles films on titania nanoparticles\/gold nanoparticles-coated Au electrode","volume":"604","author":"Shi","year":"2007","journal-title":"J. Electroanal. Chem"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jelechem.2007.02.004","article-title":"Electrochemical biosensors based on colloidal gold-carbon nanotubes composite electrodes","volume":"603","author":"Manso","year":"2007","journal-title":"J. Electroanal. Chem"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/11\/11\/10785\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:58:02Z","timestamp":1760219882000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/11\/11\/10785"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,16]]},"references-count":32,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2011,11]]}},"alternative-id":["s111110785"],"URL":"https:\/\/doi.org\/10.3390\/s111110785","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,16]]}}}