{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:29:54Z","timestamp":1767896994330,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2012,6,20]],"date-time":"2012-06-20T00:00:00Z","timestamp":1340150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A suspension protein microarray was developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles for potential applications in multiplex and high-throughput immunoassays. A simple photopatterning process produced various shapes of hydrogel micropatterns that were weakly bound to poly(dimethylsiloxane) (PDMS)-coated substrates. These micropatterns were easily detached from substrates during the washing process and were collected as non-spherical microparticles. Acrylic acids were incorporated into hydrogels, which could covalently immobilize proteins onto their surfaces due to the presence of carboxyl groups. The amount of immobilized protein increased with the amount of acrylic acid due to more available carboxyl groups. Saturation was reached at 25% v\/v of acrylic acid. Immunoassays with IgG and IgM immobilized onto hydrogel microparticles were successfully performed with a linear concentration range from 0 to 500 ng\/mL of anti-IgG and anti-IgM, respectively. Finally, a mixture of two different shapes of hydrogel microparticles immobilizing IgG (circle) and IgM (square) was prepared and it was demonstrated that simultaneous detection of two different target proteins was possible without cross-talk using same fluorescence indicator because each immunoassay was easily identified by the shapes of hydrogel microparticles.<\/jats:p>","DOI":"10.3390\/s120608426","type":"journal-article","created":{"date-parts":[[2012,6,20]],"date-time":"2012-06-20T11:57:34Z","timestamp":1340193454000},"page":"8426-8436","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Multiplex Immunoassay Platforms Based on Shape-Coded Poly(ethylene glycol) Hydrogel Microparticles Incorporating Acrylic Acid"],"prefix":"10.3390","volume":"12","author":[{"given":"Saemi","family":"Park","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Yonsei University, 134 Sinchon-Dong, Seodaemoon-Gu, Seoul 120-749, Korea"}]},{"given":"Hyun Jong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Yonsei University, 134 Sinchon-Dong, Seodaemoon-Gu, Seoul 120-749, Korea"}]},{"given":"Won-Gun","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Yonsei University, 134 Sinchon-Dong, Seodaemoon-Gu, Seoul 120-749, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2012,6,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0021-9673(03)00769-6","article-title":"Next generation of protein microarray support materials: Evaluation for protein and antibody microarray applications","volume":"1009","author":"Angenendt","year":"2003","journal-title":"J. Chromatogr. A"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1007\/s00216-008-2379-z","article-title":"Protein microarrays for diagnostic assays","volume":"393","author":"Hartmann","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S1359-6446(05)03392-1","article-title":"Progress in protein and antibody microarray technology","volume":"10","author":"Angenendt","year":"2005","journal-title":"Drug Discov. Today"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.jchromb.2003.08.034","article-title":"Protein and antibody microarray technology","volume":"797","author":"Glokler","year":"2003","journal-title":"J. Chromatogr. B"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/S1367-5931(01)00289-7","article-title":"Miniaturised multiplexed immunoassays","volume":"6","author":"Joos","year":"2001","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S34","DOI":"10.1016\/S0167-7799(01)01798-X","article-title":"Solution and chip arrays in protein profiling","volume":"19","author":"Zhou","year":"2001","journal-title":"Trends Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1002\/anie.200390150","article-title":"Recent development in protein microarray technology","volume":"42","author":"Wilson","year":"2003","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1080\/07388550600978358","article-title":"Bio-microarray fabrication techniques\u2014A review","volume":"26","author":"Lucente","year":"2006","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_9","first-page":"41","article-title":"New developments in microarray technology","volume":"12","author":"Blohm","year":"2001","journal-title":"Opin. Chem. Biol."},{"key":"ref_10","first-page":"1241","article-title":"Suspension array technology: New tools for gene and protein analysis","volume":"47","author":"Nolan","year":"2001","journal-title":"Cell. Mol. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0167-7799(01)01844-3","article-title":"Suspension array technology: Evolution of the flat-array paradigm","volume":"20","author":"Nolan","year":"2002","journal-title":"Trends Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1021\/pr060074v","article-title":"Comparison of ovalbumin quantification using forward-phase protein microarrays and suspension arrays","volume":"5","author":"Wang","year":"2006","journal-title":"J. Proteome Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"10546","DOI":"10.1021\/ja035665q","article-title":"Preparation, physical properties, on-bead binding assay and spectroscopic reliability of 25 barcoded polystyrene-poly(ethylene glycol) graft copolymers","volume":"125","author":"Fenniri","year":"2003","journal-title":"J. Am. Chem. Soc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6482","DOI":"10.1021\/la700701x","article-title":"Spectroscopically encoded microspheres for antigen biosensing","volume":"23","author":"Raez","year":"2007","journal-title":"Langmuir"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1021\/cm060283f","article-title":"Uniformly colorized beads for multiplex immunoassay","volume":"18","author":"Zhao","year":"2006","journal-title":"Chem. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1038\/90228","article-title":"Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules","volume":"19","author":"Han","year":"2001","journal-title":"Nat. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/nmat2815","article-title":"Colour-barcoded magnetic microparticles for multiplexed bioassays","volume":"9","author":"Lee","year":"2010","journal-title":"Nat. Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/nmat1617","article-title":"Continuous-flow lithography for high-throughput microparticle synthesis","volume":"5","author":"Dendukuri","year":"2006","journal-title":"Nat. Mater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1039\/b703457a","article-title":"Stop-flow lithography in a microfluidic device","volume":"7","author":"Dendukuri","year":"2007","journal-title":"Lab Chip"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1126\/science.1134929","article-title":"Multifunctional encoded particles for high-throughput biomolecule analysis","volume":"315","author":"Pregibon","year":"2007","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5851","DOI":"10.1021\/ac101032r","article-title":"Fabrication of uniform DNA-conjugated hydrogel microparticles via replica molding for facile nucleic acid hybridization assays","volume":"82","author":"Lewis","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1007\/s10544-008-9196-1","article-title":"Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays","volume":"10","author":"Lee","year":"2008","journal-title":"Biomed. Microdevices"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.1002\/adma.200803386","article-title":"The synthesis and assembly of polymeric microparticles using microfluidics","volume":"21","author":"Dendukuri","year":"2009","journal-title":"Adv. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.cocis.2011.01.005","article-title":"Hydrogel microparticles from lithographic processes: Novel materials for fundamental and applied colloid science","volume":"16","author":"Helgeson","year":"2011","journal-title":"Curr. Opin. Colloid. Interf."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1021\/ac1022343","article-title":"Multiplexed protein quantification with barcoded hydrogel microparticles","volume":"83","author":"Appleyard","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3316","DOI":"10.1021\/bm100792y","article-title":"Development of macroporous poly(ethylene glycol) hydrogel arrays within microfluidic channels","volume":"11","author":"Lee","year":"2010","journal-title":"Biomacromolecules"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.reactfunctpolym.2009.02.001","article-title":"Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning","volume":"69","author":"Park","year":"2009","journal-title":"React. Funct. Polym."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/12\/6\/8426\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:50:53Z","timestamp":1760219453000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/12\/6\/8426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,20]]},"references-count":27,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2012,6]]}},"alternative-id":["s120608426"],"URL":"https:\/\/doi.org\/10.3390\/s120608426","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,20]]}}}