{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:23:05Z","timestamp":1785820985820,"version":"3.56.0"},"reference-count":20,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2012,8,6]],"date-time":"2012-08-06T00:00:00Z","timestamp":1344211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.<\/jats:p>","DOI":"10.3390\/s120810810","type":"journal-article","created":{"date-parts":[[2012,8,6]],"date-time":"2012-08-06T11:01:08Z","timestamp":1344250868000},"page":"10810-10819","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza"],"prefix":"10.3390","volume":"12","author":[{"given":"Kyoung G.","family":"Lee","sequence":"first","affiliation":[{"name":"Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Woo","family":"Jeong","sequence":"additional","affiliation":[{"name":"Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho Woon","family":"Choi","sequence":"additional","affiliation":[{"name":"Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam Su","family":"Heo","sequence":"additional","affiliation":[{"name":"Bioprocess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Youn","family":"Park","sequence":"additional","affiliation":[{"name":"Biotechnology Research Division, National Fisheries Research & Development Institute (NFRDI), 408-1 Sirang-ri, Gijang, Busan 619-705, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8918-0957","authenticated-orcid":false,"given":"Tae Jung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2012,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1016\/j.bios.2010.08.076","article-title":"Multiplexed detection and differentiation of the DNA strains for influenza A (H1N1 2009) using a silicon-based microfluidic system","volume":"26","author":"Kao","year":"2011","journal-title":"Biosens. 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Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1016\/j.bios.2008.09.004","article-title":"On-chip detection of myoglobin based on fluorescence","volume":"24","author":"Darain","year":"2009","journal-title":"Biosens. Bioelectron"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.aca.2008.01.064","article-title":"Current development in microfluidic immunosensing chip","volume":"611","author":"Henares","year":"2008","journal-title":"Anal. Chim. Acta."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"034104","DOI":"10.1063\/1.3609968","article-title":"Electrophoretic separation of neurotransmitters on a polystyrene nano-sphere\/polystyrene sulphonate coated poly(dimethylsiloxane) microchannel","volume":"5","author":"Zhao","year":"2011","journal-title":"Biomicrofluidics"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s10404-010-0605-4","article-title":"Cyclic olefin polymers: Emerging materials for lab-on-a-chip applications","volume":"9","author":"Nunes","year":"2010","journal-title":"Microfluid. Nanofluid"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2592","DOI":"10.1016\/j.bios.2009.01.030","article-title":"Directed self-assembly of gold binding polypeptide-protein: A fusion proteins for development of gold nanoparticle-based SPR immunosensors","volume":"24","author":"Ko","year":"2009","journal-title":"Biosens. Bioelectron"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.bios.2008.06.045","article-title":"Towards on-site pathogen detection using antibody-based sensors","volume":"24","author":"Skottrup","year":"2008","journal-title":"Biosens. Bioelectron"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/nbt0397-269","article-title":"Metal-recongnition by repeating polypeptide","volume":"15","author":"Brown","year":"1997","journal-title":"Nat. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7197","DOI":"10.1021\/ac060976f","article-title":"Protein nanopatterns and biosensors using gold binding polypeptide as a fusion partner","volume":"78","author":"Park","year":"2006","journal-title":"Anal. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1002\/elps.200700823","article-title":"Development of a fully integrated microfluidic system for sensing infectious viral disease","volume":"29","author":"Huh","year":"2008","journal-title":"Electrophoresis"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.talanta.2009.03.051","article-title":"A self-assembled fusion protein-based surface plasmon resonance biosensor for rapid diagnosis of severe acute respiratory syndrome","volume":"79","author":"Park","year":"2009","journal-title":"Talanta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1002\/elan.200900556","article-title":"Development of the glucose biosensor using advanced electrode modified by nanohybrid composing chemically modified graphene and ionic liquid","volume":"22","author":"Yang","year":"2010","journal-title":"Electroanalysis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/j.talanta.2010.05.059","article-title":"Label-free optical diagnosis of hepaptitis b virus with genetically engineered fusion proteins","volume":"82","author":"Zeng","year":"2010","journal-title":"Talanta"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3999","DOI":"10.1039\/c1lc20441f","article-title":"Sustainable fabrication of micro-structured lab-on-a-chip","volume":"11","author":"Oh","year":"2011","journal-title":"Lab Chip"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6374","DOI":"10.1039\/c0cc00762e","article-title":"Synthesis and characterization of gold-deposited red, green and blue fluorescent silica nanoparticles for biosensing application","volume":"46","author":"Lee","year":"2010","journal-title":"Chem. 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Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.snb.2005.04.030","article-title":"Mono and dithiol surfaces on surface plasmon resonance biosensors for detection of staphylococcus aureus","volume":"114","author":"Subramanian","year":"2006","journal-title":"Sensor Actuator B Chem."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/12\/8\/10810\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:51:38Z","timestamp":1760219498000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/12\/8\/10810"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,6]]},"references-count":20,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2012,8]]}},"alternative-id":["s120810810"],"URL":"https:\/\/doi.org\/10.3390\/s120810810","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,6]]}}}