{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:36:12Z","timestamp":1760243772170,"version":"build-2065373602"},"reference-count":22,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Visible and near infrared (Vis\/NIR) spectroscopy were employed for the fast and nondestructive estimation of the total amino acid (TAA) content in barley (Hordeum vulgare L.) leaves. The calibration set was composed of 50 samples; and the remaining 25 samples were used for the validation set. Seven different spectral preprocessing methods and six different calibration methods (linear and nonlinear) were applied for a comprehensive prediction performance comparison. Successive projections algorithm (SPA) and regression coefficients (RC) were applied to select effective wavelengths (EWs). The results indicated that the latent variables-least-squares-support vector machine (LV-LS-SVM) model achieved the optimal performance. The prediction results by LV-LS-SVM with raw spectra were achieved with a correlation coefficients (r) = 0.937 and root mean squares error of prediction (RMSEP) = 0.530. The overall results showed that the NIR spectroscopy could be used for determination of TAA content in barley leaves with an excellent prediction precision; and the results were also helpful for on-field monitoring of barley growing status under herbicide stress during different growth stages.<\/jats:p>","DOI":"10.3390\/s121013393","type":"journal-article","created":{"date-parts":[[2012,10,2]],"date-time":"2012-10-02T02:39:08Z","timestamp":1349145548000},"page":"13393-13401","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Quantitative Analysis of Total Amino Acid in Barley Leaves under Herbicide Stress Using Spectroscopic Technology and Chemometrics"],"prefix":"10.3390","volume":"12","author":[{"given":"Yidan","family":"Bao","sequence":"first","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwen","family":"Kong","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6752-1757","authenticated-orcid":false,"given":"Yong","family":"He","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0266-6896","authenticated-orcid":false,"given":"Fei","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weijun","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2012,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6493","DOI":"10.1021\/jf301457k","article-title":"Compositional equivalence of barleys differing only in low-and Normal-phytate levels","volume":"60","author":"Moreau","year":"2012","journal-title":"Agric. 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