{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T03:44:16Z","timestamp":1770435856729,"version":"3.49.0"},"reference-count":41,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T00:00:00Z","timestamp":1358294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>With an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possesses an unlabelled G-quadruplex DNAzyme molecular beacon (MB) probe, employing complementary sequence as biorecognition element and 1:1:1:1 split G-quadruplex halves as reporter. In the absence of target DNA (T-DNA), the probe can shape intermolecular G-quadruplex structures capable of binding hemin to form G-quadruplex-hemin DNAzyme and catalyze the oxidation of ABTS2\u2212 to blue-green ABTS\u2022\u2212 by H2O2. In the presence of T-DNA, T-DNA can hybridize with the complementary sequence to form a duplex structure, hindering the formation of the G-quadruplex structure and resulting in the loss of the catalytic activity. Consequently, a UV-Vis absorption signal decrease is observed in the ABTS2\u2212-H2O2 system. The \u201cturn-off\u201d assay allows the detection of T-DNA from 1.0 \u00d7 10\u22129 to 3.0 \u00d7 10\u22127 mol\u00b7L\u22121 (R2 = 0.9906), with a low detection limit of 3.1 \u00d7 10\u221210 mol\u00b7L\u22121. The present study provides a sensitive and selective method and may serve as a foundation of utilizing the DNAzyme MB sensor for identifying traditional Chinese medicines.<\/jats:p>","DOI":"10.3390\/s130101064","type":"journal-article","created":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T11:20:29Z","timestamp":1358335229000},"page":"1064-1075","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["G-Quadruplex DNAzyme Molecular Beacon for Amplified Colorimetric Biosensing of Pseudostellaria heterophylla"],"prefix":"10.3390","volume":"13","author":[{"given":"Zhenzhu","family":"Zheng","sequence":"first","affiliation":[{"name":"Institute of Drug Research, Fujian Academy of Chinese Medicine, Fuzhou 350003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Drug Research, Fujian Academy of Chinese Medicine, Fuzhou 350003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wensheng","family":"Pang","sequence":"additional","affiliation":[{"name":"The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China"},{"name":"The Second People's Hospital of Fujian Province, Fuzhou 350003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4747-7503","authenticated-orcid":false,"given":"Juan","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Drug Research, Fujian Academy of Chinese Medicine, Fuzhou 350003, China"},{"name":"The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1021\/np50060a030","article-title":"Phytochemical studies of the Chinese Herb Ta-zi-shen, Pseudostellaria heterophylla","volume":"51","author":"Reinecke","year":"1988","journal-title":"J. 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