{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T17:01:22Z","timestamp":1763226082780,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T00:00:00Z","timestamp":1361923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>This study demonstrated the feasibility of utilizing electrokinesis in an electrodeless dielectrophoresis chip to separate and concentrate microparticles such as biosamples. Numerical simulations and experimental observations were facilitated to investigate the phenomena of electrokinetics, i.e., electroosmosis, dielectrophoresis, and electrothermosis. Moreover, the proposed operating mode can be used to simultaneously convey microparticles through a microfluidic device by using electroosmotic flow, eliminating the need for an additional micropump. These results not only revealed that the directions of fluids could be controlled with a forward\/backward electroosmotic flow but also categorized the optimum separating parameters for various microparticle sizes  (0.5, 1.0 and 2.0 \u03bcm). Separation of microparticles can be achieved by tuning driving frequencies at a specific electric potential (90 Vpp\u00b7cm\u22121). Certainly, the device can be designed as a single automated device that carries out multiple functions such as transportation, separation, and detection for the realization of the envisioned  Lab-on-a-Chip idea.<\/jats:p>","DOI":"10.3390\/s130302763","type":"journal-article","created":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T11:43:57Z","timestamp":1361965437000},"page":"2763-2776","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of Microparticle Separation Utilizing Electrokinesis within an Electrodeless Dielectrophoresis Chip"],"prefix":"10.3390","volume":"13","author":[{"given":"Chi-Han","family":"Chiou","sequence":"first","affiliation":[{"name":"ITRI South Campus, Industrial Technology Research Institute, Tainan 70955, Taiwan"}]},{"given":"Jia-Cheng","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Automation Engineering, I-Shou University, Kaohsiung 84001, Taiwan"}]},{"given":"Liang-Ju","family":"Chien","sequence":"additional","affiliation":[{"name":"ITRI South Campus, Industrial Technology Research Institute, Tainan 70955, Taiwan"}]},{"given":"Yu-Ying","family":"Lin","sequence":"additional","affiliation":[{"name":"ITRI South Campus, Industrial Technology Research Institute, Tainan 70955, Taiwan"}]},{"given":"Jr-Lung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Automation Engineering, I-Shou University, Kaohsiung 84001, Taiwan"}]}],"member":"1968","published-online":{"date-parts":[[2013,2,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1002\/elps.200390087","article-title":"Microdevices for manipulation and accumulation of micro- and nanoparticles by dielectrophoresis","volume":"24","author":"Durr","year":"2003","journal-title":"Electrophoresis"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3883","DOI":"10.1002\/1522-2683(200110)22:18<3883::AID-ELPS3883>3.0.CO;2-4","article-title":"Design of a microfabricated magnetic cell separator","volume":"22","author":"Berger","year":"2001","journal-title":"Electrophoresis"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1039\/b401834f","article-title":"Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells","volume":"4","author":"Zhu","year":"2004","journal-title":"Lab Chip."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1109\/28.54263","article-title":"Handling biological cells using a fluid integrated circuit","volume":"26","author":"Washizu","year":"1990","journal-title":"IEEE Trans. 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